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Detection of herpes simplex virus DNA in atypical epithelial keratitis using polymerase chain reaction.
Br J Ophthalmol 1999; 83(8):957-60BJ

Abstract

AIM

To study herpes simplex virus (HSV) DNA in tears from patients with atypical epithelial keratitis of unknown aetiology.

METHODS

Tear samples were collected from 17 affected eyes of 17 consecutive patients suffering from epithelial keratitis in whom HSV keratitis was suspected but whose diagnosis was difficult on the basis of clinical manifestations alone. Using reduced sensitivity polymerase chain reaction (PCR), tear samples were tested for HSV DNA. Tears from the unaffected eyes of the 17 patients were also examined, along with 38 tear samples from 19 normal volunteers. Southern blot analysis was performed to confirm that amplified DNA bands were specific for HSV. Clinical correlation with photographs of corneal lesions was also investigated.

RESULTS

HSV DNA was detected in tears from the affected eyes of eight of the 17 patients with suspected HSV keratitis. Tears from the affected eyes of the other patients were PCR negative, as were tears from the unaffected eyes of all 17 patients, and from the 38 normal eyes. There was no correlation between PCR results and clinical manifestation of keratitis.

CONCLUSIONS

Based on the sensitivity of the PCR system, eight of 17 suspected HSV keratitis patients were confirmed as suffering from HSV keratitis. HSV keratitis should therefore be considered as a possible diagnosis in atypical epithelial keratitis.

Authors+Show Affiliations

Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10413702

Citation

Koizumi, N, et al. "Detection of Herpes Simplex Virus DNA in Atypical Epithelial Keratitis Using Polymerase Chain Reaction." The British Journal of Ophthalmology, vol. 83, no. 8, 1999, pp. 957-60.
Koizumi N, Nishida K, Adachi W, et al. Detection of herpes simplex virus DNA in atypical epithelial keratitis using polymerase chain reaction. Br J Ophthalmol. 1999;83(8):957-60.
Koizumi, N., Nishida, K., Adachi, W., Tei, M., Honma, Y., Dota, A., ... Kinoshita, S. (1999). Detection of herpes simplex virus DNA in atypical epithelial keratitis using polymerase chain reaction. The British Journal of Ophthalmology, 83(8), pp. 957-60.
Koizumi N, et al. Detection of Herpes Simplex Virus DNA in Atypical Epithelial Keratitis Using Polymerase Chain Reaction. Br J Ophthalmol. 1999;83(8):957-60. PubMed PMID: 10413702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of herpes simplex virus DNA in atypical epithelial keratitis using polymerase chain reaction. AU - Koizumi,N, AU - Nishida,K, AU - Adachi,W, AU - Tei,M, AU - Honma,Y, AU - Dota,A, AU - Sotozono,C, AU - Yokoi,N, AU - Yamamoto,S, AU - Kinoshita,S, PY - 1999/7/22/pubmed PY - 1999/7/22/medline PY - 1999/7/22/entrez SP - 957 EP - 60 JF - The British journal of ophthalmology JO - Br J Ophthalmol VL - 83 IS - 8 N2 - AIM: To study herpes simplex virus (HSV) DNA in tears from patients with atypical epithelial keratitis of unknown aetiology. METHODS: Tear samples were collected from 17 affected eyes of 17 consecutive patients suffering from epithelial keratitis in whom HSV keratitis was suspected but whose diagnosis was difficult on the basis of clinical manifestations alone. Using reduced sensitivity polymerase chain reaction (PCR), tear samples were tested for HSV DNA. Tears from the unaffected eyes of the 17 patients were also examined, along with 38 tear samples from 19 normal volunteers. Southern blot analysis was performed to confirm that amplified DNA bands were specific for HSV. Clinical correlation with photographs of corneal lesions was also investigated. RESULTS: HSV DNA was detected in tears from the affected eyes of eight of the 17 patients with suspected HSV keratitis. Tears from the affected eyes of the other patients were PCR negative, as were tears from the unaffected eyes of all 17 patients, and from the 38 normal eyes. There was no correlation between PCR results and clinical manifestation of keratitis. CONCLUSIONS: Based on the sensitivity of the PCR system, eight of 17 suspected HSV keratitis patients were confirmed as suffering from HSV keratitis. HSV keratitis should therefore be considered as a possible diagnosis in atypical epithelial keratitis. SN - 0007-1161 UR - https://www.unboundmedicine.com/medline/citation/10413702/Detection_of_herpes_simplex_virus_DNA_in_atypical_epithelial_keratitis_using_polymerase_chain_reaction_ L2 - http://bjo.bmj.com/cgi/pmidlookup?view=long&pmid=10413702 DB - PRIME DP - Unbound Medicine ER -