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Liquid chromatographic bioanalytical determination of ropivacaine, bupivacaine and major metabolites.
Biomed Chromatogr. 1999 Jun; 13(4):286-92.BC

Abstract

Bioanalytical methods for the determination of ropivacaine, bupivacaine and their major metabolites in urine and blood plasma are presented. Ropivacaine is a new local anaesthetic drug mainly used for surgery and for postoperative pain relief. The samples are hydrolysed and cleaned using solid-phase extraction and analysed using ion-pair reversed-phase liquid chromatography with gradient elution. The analytes are detected using UV at 210 nm. The methods are highly selective and the limits of quantification were 1 microM in urine and 0.1 microM in plasma, respectively. The between-day variance was generally below 3% (RSD).

Authors+Show Affiliations

Astra Pain Control AB, Concept Division, Södertälje, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10416062

Citation

Arvidsson, T, et al. "Liquid Chromatographic Bioanalytical Determination of Ropivacaine, Bupivacaine and Major Metabolites." Biomedical Chromatography : BMC, vol. 13, no. 4, 1999, pp. 286-92.
Arvidsson T, Askemark Y, Halldin MM. Liquid chromatographic bioanalytical determination of ropivacaine, bupivacaine and major metabolites. Biomed Chromatogr. 1999;13(4):286-92.
Arvidsson, T., Askemark, Y., & Halldin, M. M. (1999). Liquid chromatographic bioanalytical determination of ropivacaine, bupivacaine and major metabolites. Biomedical Chromatography : BMC, 13(4), 286-92.
Arvidsson T, Askemark Y, Halldin MM. Liquid Chromatographic Bioanalytical Determination of Ropivacaine, Bupivacaine and Major Metabolites. Biomed Chromatogr. 1999;13(4):286-92. PubMed PMID: 10416062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatographic bioanalytical determination of ropivacaine, bupivacaine and major metabolites. AU - Arvidsson,T, AU - Askemark,Y, AU - Halldin,M M, PY - 1999/7/23/pubmed PY - 2000/6/20/medline PY - 1999/7/23/entrez SP - 286 EP - 92 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 13 IS - 4 N2 - Bioanalytical methods for the determination of ropivacaine, bupivacaine and their major metabolites in urine and blood plasma are presented. Ropivacaine is a new local anaesthetic drug mainly used for surgery and for postoperative pain relief. The samples are hydrolysed and cleaned using solid-phase extraction and analysed using ion-pair reversed-phase liquid chromatography with gradient elution. The analytes are detected using UV at 210 nm. The methods are highly selective and the limits of quantification were 1 microM in urine and 0.1 microM in plasma, respectively. The between-day variance was generally below 3% (RSD). SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/10416062/Liquid_chromatographic_bioanalytical_determination_of_ropivacaine_bupivacaine_and_major_metabolites_ L2 - https://doi.org/10.1002/(SICI)1099-0801(199906)13:4<286::AID-BMC862>3.0.CO;2-M DB - PRIME DP - Unbound Medicine ER -