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[PCR SSCP study of an echovirus 30 meningitis outbreak].
Pathol Biol (Paris). 1999 May; 47(5):534-8.PB

Abstract

Between April and October 1997, 21 children of 4 days to 13 years old were admitted to the Pedatric Unit of Aulnay Sous Bois's Hospital for viral meningitidis. The number of white blood cells in the cerebrospinal fluid (CSF) was between 1 and 612 cells/mm3, with, on an average, 56% of segmented cells, 34% lymphocytes and 34% monocytes. Proteins and glucose of CSF were standard. One CSF was normal. Viral meningitidis was confirmed by viral culture of CSF onto MRC5. Enterovirus were identified by direct immunofluorescence (Monoclonal Mouse Anti-Enterovirus, Dako). Serotyping (Enterovirus antisera, Eurobio, Trousses 4) identified an echovirus 30 in all cases. A highly conserved 154 bp sequence at the 5'non-coding region was studied by reverse transcription-polymerase chain reaction (RT-PCR) followed by single-strand conformation polymorphism (SSCP) (GenPhor, Pharmacia) analysis. Two dominant SSCP patterns were observed: the first contained 4/21 strains and the other 10/21 strains. The SSCP patterns of the 7 other strains were different. These results show that 2 echovirus 30 dominant clones were responsible of viral meningitidis admitted to the Pediatric Unit of Aulnay Sous Bois's hospital, between april and october 1997. The PCR-SSCP of the 5'non-coding region of echovirus 30 is a convenient, simple, reproducible epidemiologic method and it's easily applicable in a general hospital.

Authors+Show Affiliations

Service de Biologie Médicale, CH Robert-Ballanger, Boulevard Robert-Ballanger, Aulnay-Sous-Bois, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

fre

PubMed ID

10418035

Citation

Maisonneuve, L, et al. "[PCR SSCP Study of an Echovirus 30 Meningitis Outbreak]." Pathologie-biologie, vol. 47, no. 5, 1999, pp. 534-8.
Maisonneuve L, Esteve V, Scart G, et al. [PCR SSCP study of an echovirus 30 meningitis outbreak]. Pathol Biol (Paris). 1999;47(5):534-8.
Maisonneuve, L., Esteve, V., Scart, G., Lusina, D., Le Pennec, M. P., Baledent, F., & Bingen, E. (1999). [PCR SSCP study of an echovirus 30 meningitis outbreak]. Pathologie-biologie, 47(5), 534-8.
Maisonneuve L, et al. [PCR SSCP Study of an Echovirus 30 Meningitis Outbreak]. Pathol Biol (Paris). 1999;47(5):534-8. PubMed PMID: 10418035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [PCR SSCP study of an echovirus 30 meningitis outbreak]. AU - Maisonneuve,L, AU - Esteve,V, AU - Scart,G, AU - Lusina,D, AU - Le Pennec,M P, AU - Baledent,F, AU - Bingen,E, PY - 1999/7/27/pubmed PY - 1999/7/27/medline PY - 1999/7/27/entrez SP - 534 EP - 8 JF - Pathologie-biologie JO - Pathol Biol (Paris) VL - 47 IS - 5 N2 - Between April and October 1997, 21 children of 4 days to 13 years old were admitted to the Pedatric Unit of Aulnay Sous Bois's Hospital for viral meningitidis. The number of white blood cells in the cerebrospinal fluid (CSF) was between 1 and 612 cells/mm3, with, on an average, 56% of segmented cells, 34% lymphocytes and 34% monocytes. Proteins and glucose of CSF were standard. One CSF was normal. Viral meningitidis was confirmed by viral culture of CSF onto MRC5. Enterovirus were identified by direct immunofluorescence (Monoclonal Mouse Anti-Enterovirus, Dako). Serotyping (Enterovirus antisera, Eurobio, Trousses 4) identified an echovirus 30 in all cases. A highly conserved 154 bp sequence at the 5'non-coding region was studied by reverse transcription-polymerase chain reaction (RT-PCR) followed by single-strand conformation polymorphism (SSCP) (GenPhor, Pharmacia) analysis. Two dominant SSCP patterns were observed: the first contained 4/21 strains and the other 10/21 strains. The SSCP patterns of the 7 other strains were different. These results show that 2 echovirus 30 dominant clones were responsible of viral meningitidis admitted to the Pediatric Unit of Aulnay Sous Bois's hospital, between april and october 1997. The PCR-SSCP of the 5'non-coding region of echovirus 30 is a convenient, simple, reproducible epidemiologic method and it's easily applicable in a general hospital. SN - 0369-8114 UR - https://www.unboundmedicine.com/medline/citation/10418035/[PCR_SSCP_study_of_an_echovirus_30_meningitis_outbreak]_ DB - PRIME DP - Unbound Medicine ER -