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A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice.
Dev Biol. 1999 Aug 01; 212(1):191-203.DB

Abstract

Neural crest cells are embryonic, multipotent stem cells that give rise to various cell/tissue types and thus serve as a good model system for the study of cell specification and mechanisms of cell differentiation. For analysis of neural crest cell lineage, an efficient method has been devised for manipulating the mouse genome through the Cre-loxP system. We generated transgenic mice harboring a Cre gene driven by a promoter of protein 0 (P0). To detect the Cre-mediated DNA recombination, we crossed P0-Cre transgenic mice with CAG-CAT-Z indicator transgenic mice. The CAG-CAT-Z Tg line carries a lacZ gene downstream of a chicken beta-actin promoter and a "stuffer" fragment flanked by two loxP sequences, so that lacZ is expressed only when the stuffer is removed by the action of Cre recombinase. In three different P0-Cre lines crossed with CAG-CAT-Z Tg, embryos carrying both transgenes showed lacZ expression in tissues derived from neural crest cells, such as spinal dorsal root ganglia, sympathetic nervous system, enteric nervous system, and ventral craniofacial mesenchyme at stages later than 9.0 dpc. These findings give some insights into neural crest cell differentiation in mammals. We believe that P0-Cre transgenic mice will facilitate many interesting experiments, including lineage analysis, purification, and genetic manipulation of the mammalian neural crest cells.

Authors+Show Affiliations

Institute of Molecular Embryology and Genetics, Center for Animal Resources and Development, Kumamoto University School of Medicine, 4-24-1 Kuhonji, Kumamoto, 862-0976, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10419695

Citation

Yamauchi, Y, et al. "A Novel Transgenic Technique That Allows Specific Marking of the Neural Crest Cell Lineage in Mice." Developmental Biology, vol. 212, no. 1, 1999, pp. 191-203.
Yamauchi Y, Abe K, Mantani A, et al. A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice. Dev Biol. 1999;212(1):191-203.
Yamauchi, Y., Abe, K., Mantani, A., Hitoshi, Y., Suzuki, M., Osuzu, F., Kuratani, S., & Yamamura, K. (1999). A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice. Developmental Biology, 212(1), 191-203.
Yamauchi Y, et al. A Novel Transgenic Technique That Allows Specific Marking of the Neural Crest Cell Lineage in Mice. Dev Biol. 1999 Aug 1;212(1):191-203. PubMed PMID: 10419695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice. AU - Yamauchi,Y, AU - Abe,K, AU - Mantani,A, AU - Hitoshi,Y, AU - Suzuki,M, AU - Osuzu,F, AU - Kuratani,S, AU - Yamamura,K, PY - 1999/7/27/pubmed PY - 1999/7/27/medline PY - 1999/7/27/entrez SP - 191 EP - 203 JF - Developmental biology JO - Dev Biol VL - 212 IS - 1 N2 - Neural crest cells are embryonic, multipotent stem cells that give rise to various cell/tissue types and thus serve as a good model system for the study of cell specification and mechanisms of cell differentiation. For analysis of neural crest cell lineage, an efficient method has been devised for manipulating the mouse genome through the Cre-loxP system. We generated transgenic mice harboring a Cre gene driven by a promoter of protein 0 (P0). To detect the Cre-mediated DNA recombination, we crossed P0-Cre transgenic mice with CAG-CAT-Z indicator transgenic mice. The CAG-CAT-Z Tg line carries a lacZ gene downstream of a chicken beta-actin promoter and a "stuffer" fragment flanked by two loxP sequences, so that lacZ is expressed only when the stuffer is removed by the action of Cre recombinase. In three different P0-Cre lines crossed with CAG-CAT-Z Tg, embryos carrying both transgenes showed lacZ expression in tissues derived from neural crest cells, such as spinal dorsal root ganglia, sympathetic nervous system, enteric nervous system, and ventral craniofacial mesenchyme at stages later than 9.0 dpc. These findings give some insights into neural crest cell differentiation in mammals. We believe that P0-Cre transgenic mice will facilitate many interesting experiments, including lineage analysis, purification, and genetic manipulation of the mammalian neural crest cells. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/10419695/A_novel_transgenic_technique_that_allows_specific_marking_of_the_neural_crest_cell_lineage_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012160699993235 DB - PRIME DP - Unbound Medicine ER -