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Tear secretory IgA: evaluation of usefulness as a diagnostic marker in herpetic keratitis.
Ocul Immunol Inflamm 1999; 7(2):61-7OI

Abstract

In a South Indian study, an 'in-house' enzyme-linked immunosorbent assay (ELISA) was developed to evaluate the potential of herpes simplex virus (HSV)-specific tear secretory IgA (sIgA) in the diagnosis of herpes simplex keratitis (HSK). The presence of HSV-specific tear sIgA was found to be diagnostic in 20.28% of cases. The usefulness of the sIgA ELISA system was evaluated against HSV isolation, which is the 'gold standard' and HSV antigen detection, a more sensitive, commonly employed method. Analysis of HSV-specific IgG and IgM results showed their failure as reliable indicators of active or ongoing infection. Comparison of sIgA ELISA with culture as 'gold standard' showed its sensitivity, specificity, and positive and negative predictive values to be 60% (95% CI 36.4-80), 93.2% (95% CI 86.7-96.8), 60% (95% CI 36.4-80), and 93.2% (95% CI 86.7-96. 8), respectively. This study is the first report on the complete evaluation of the usefulness of tear anti-HSV sIgA in the laboratory diagnosis of HSK, taking into account both epithelial and stromal keratitis cases.

Authors+Show Affiliations

Dr. ALM Post Graduate Institute of Basic Medical Sciences University of Madras Department of Microbiology India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10420200

Citation

Pramod, N P., et al. "Tear Secretory IgA: Evaluation of Usefulness as a Diagnostic Marker in Herpetic Keratitis." Ocular Immunology and Inflammation, vol. 7, no. 2, 1999, pp. 61-7.
Pramod NP, Dhevahi E, Sudhamathi K, et al. Tear secretory IgA: evaluation of usefulness as a diagnostic marker in herpetic keratitis. Ocul Immunol Inflamm. 1999;7(2):61-7.
Pramod, N. P., Dhevahi, E., Sudhamathi, K., Kannan, K., & Thyagarajan, S. P. (1999). Tear secretory IgA: evaluation of usefulness as a diagnostic marker in herpetic keratitis. Ocular Immunology and Inflammation, 7(2), pp. 61-7.
Pramod NP, et al. Tear Secretory IgA: Evaluation of Usefulness as a Diagnostic Marker in Herpetic Keratitis. Ocul Immunol Inflamm. 1999;7(2):61-7. PubMed PMID: 10420200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tear secretory IgA: evaluation of usefulness as a diagnostic marker in herpetic keratitis. AU - Pramod,N P, AU - Dhevahi,E, AU - Sudhamathi,K, AU - Kannan,K, AU - Thyagarajan,S P, PY - 1999/7/27/pubmed PY - 1999/7/27/medline PY - 1999/7/27/entrez SP - 61 EP - 7 JF - Ocular immunology and inflammation JO - Ocul. Immunol. Inflamm. VL - 7 IS - 2 N2 - In a South Indian study, an 'in-house' enzyme-linked immunosorbent assay (ELISA) was developed to evaluate the potential of herpes simplex virus (HSV)-specific tear secretory IgA (sIgA) in the diagnosis of herpes simplex keratitis (HSK). The presence of HSV-specific tear sIgA was found to be diagnostic in 20.28% of cases. The usefulness of the sIgA ELISA system was evaluated against HSV isolation, which is the 'gold standard' and HSV antigen detection, a more sensitive, commonly employed method. Analysis of HSV-specific IgG and IgM results showed their failure as reliable indicators of active or ongoing infection. Comparison of sIgA ELISA with culture as 'gold standard' showed its sensitivity, specificity, and positive and negative predictive values to be 60% (95% CI 36.4-80), 93.2% (95% CI 86.7-96.8), 60% (95% CI 36.4-80), and 93.2% (95% CI 86.7-96. 8), respectively. This study is the first report on the complete evaluation of the usefulness of tear anti-HSV sIgA in the laboratory diagnosis of HSK, taking into account both epithelial and stromal keratitis cases. SN - 0927-3948 UR - https://www.unboundmedicine.com/medline/citation/10420200/Tear_secretory_IgA:_evaluation_of_usefulness_as_a_diagnostic_marker_in_herpetic_keratitis_ L2 - http://www.diseaseinfosearch.org/result/3400 DB - PRIME DP - Unbound Medicine ER -