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Extracellular polysaccharide of Erwinia chrysanthemi CU643.
Carbohydr Res. 1999 Mar 31; 316(1-4):138-54.CR

Abstract

Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit: -->3)-beta-D-Galp-(1-->2)-alpha-L-Rhap-(1-->4)-beta-D-GlcAp- (1-->2)-alpha-L- Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->(1).

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Publisher Full Text

Authors+Show Affiliations

Yang BY
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA.
Gray JS
No affiliation info available
Montgomery R
No affiliation info available

MeSH

Carbohydrate SequenceChromatography, GelChromatography, Ion ExchangeDickeya chrysanthemiMagnetic Resonance SpectroscopyMethylationMolecular Sequence DataPolysaccharides, BacterialSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10420593

Citation

Yang, B Y., et al. "Extracellular Polysaccharide of Erwinia Chrysanthemi CU643." Carbohydrate Research, vol. 316, no. 1-4, 1999, pp. 138-54.
Yang BY, Gray JS, Montgomery R. Extracellular polysaccharide of Erwinia chrysanthemi CU643. Carbohydr Res. 1999;316(1-4):138-54.
Yang, B. Y., Gray, J. S., & Montgomery, R. (1999). Extracellular polysaccharide of Erwinia chrysanthemi CU643. Carbohydrate Research, 316(1-4), 138-54.
Yang BY, Gray JS, Montgomery R. Extracellular Polysaccharide of Erwinia Chrysanthemi CU643. Carbohydr Res. 1999 Mar 31;316(1-4):138-54. PubMed PMID: 10420593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extracellular polysaccharide of Erwinia chrysanthemi CU643. AU - Yang,B Y, AU - Gray,J S, AU - Montgomery,R, PY - 1999/7/27/pubmed PY - 1999/7/27/medline PY - 1999/7/27/entrez SP - 138 EP - 54 JF - Carbohydrate research JO - Carbohydr Res VL - 316 IS - 1-4 N2 - Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit: -->3)-beta-D-Galp-(1-->2)-alpha-L-Rhap-(1-->4)-beta-D-GlcAp- (1-->2)-alpha-L- Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->(1). SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/10420593/Extracellular_polysaccharide_of_Erwinia_chrysanthemi_CU643_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-6215(99)00054-3 DB - PRIME DP - Unbound Medicine ER -