Tags

Type your tag names separated by a space and hit enter

Properties of ventricular myocytes isolated from the hypertrophied and failing hearts of spontaneously hypertensive rats.
J Physiol Pharmacol. 1999 Jun; 50(2):243-58.JP

Abstract

OBJECTIVE

To investigate how the morphological and physiological properties of single myocytes isolated from the hypertrophied, failing left ventricles (LV) differ from those of normal or hypertrophied not failing ventricles.

METHOD

Single myocytes were isolated separately from right (RV) and left ventricles (LV) of male spontaneously hypertensive rats (SHR) or Wistar-Kyoto (WKY) rats at the age of 6 and 12 months and of SHRs which developed or not developed heart failure at the age of 20-24 months. We measured cells dimensions, range and kinetics of electrically stimulated or initiated by caffeine contractions and Ca2+ transients, and investigated the response of cells to thapsigargin.

RESULTS

The transversal dimensions of the LV myocytes of 6 months old SHRs showed approximately 20% increase with respect to transversal dimensions of their RV myocytes and LV and RV myocytes of WKY rats. The difference did not change with progressing age and in the heart failure. The LV myocytes of 6 or 12 months old SHRs showed slowed kinetics of the Ca2+ transients and of contraction and relaxation and decreased contractile response to 2 s superfusion with 15 mM caffeine preceded by 5 mM Ni2+ used as an index of the sarcoplasmic reticulum (SR) Ca2+ content. Despite of this the range of shortening and relative contribution of the SR to contraction (assessed by measuring of the residual contractile response to electrical stimulation in cells poisoned with thapsigargin) or relaxation (assessed by calculation of the ratio of rate constants of the electrically stimulated and stimulated by 30 s superfusion with caffeine Ca2+ transients) was not altered in the hypertrophied myocytes. Properties of the LV myocytes of the 20-24 old SHRs with or without heart failure did not differ from those of LV myocytes of younger SHRs. The contractile response to caffeine of their RV myocytes dropped to the level of that in the LV myocytes.

CONCLUSION

Our results suggest that transition from the compensated hypertrophy to the heart failure in 20-24 months old SHRs did not result from the further changes in properties of the surviving myocytes. Data from literature suggest that myocyte apoptosis and remodeling of the extramyocyte space is the more likely reason.

Authors+Show Affiliations

Emanuel K
Department of Clinical Physiology, Medical Center of Postgraduate Education, Warsaw, Poland.
Mackiewicz U
No affiliation info available
Pytkowski B
No affiliation info available
Lewartowski B
No affiliation info available

MeSH

AnimalsApoptosisCaffeineCalciumCell CountCell SizeCentral Nervous System StimulantsElectric StimulationEnzyme InhibitorsHeart FailureHypertrophy, Left VentricularMaleMuscle Fibers, SkeletalMuscle ProteinsRatsRats, Inbred SHRRats, Inbred WKYSarcoplasmic ReticulumThapsigarginVentricular Dysfunction, LeftVentricular Function, Right

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10424720

Citation

Emanuel, K, et al. "Properties of Ventricular Myocytes Isolated From the Hypertrophied and Failing Hearts of Spontaneously Hypertensive Rats." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 50, no. 2, 1999, pp. 243-58.
Emanuel K, Mackiewicz U, Pytkowski B, et al. Properties of ventricular myocytes isolated from the hypertrophied and failing hearts of spontaneously hypertensive rats. J Physiol Pharmacol. 1999;50(2):243-58.
Emanuel, K., Mackiewicz, U., Pytkowski, B., & Lewartowski, B. (1999). Properties of ventricular myocytes isolated from the hypertrophied and failing hearts of spontaneously hypertensive rats. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 50(2), 243-58.
Emanuel K, et al. Properties of Ventricular Myocytes Isolated From the Hypertrophied and Failing Hearts of Spontaneously Hypertensive Rats. J Physiol Pharmacol. 1999;50(2):243-58. PubMed PMID: 10424720.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Properties of ventricular myocytes isolated from the hypertrophied and failing hearts of spontaneously hypertensive rats. AU - Emanuel,K, AU - Mackiewicz,U, AU - Pytkowski,B, AU - Lewartowski,B, PY - 1999/7/29/pubmed PY - 1999/7/29/medline PY - 1999/7/29/entrez SP - 243 EP - 58 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J Physiol Pharmacol VL - 50 IS - 2 N2 - OBJECTIVE: To investigate how the morphological and physiological properties of single myocytes isolated from the hypertrophied, failing left ventricles (LV) differ from those of normal or hypertrophied not failing ventricles. METHOD: Single myocytes were isolated separately from right (RV) and left ventricles (LV) of male spontaneously hypertensive rats (SHR) or Wistar-Kyoto (WKY) rats at the age of 6 and 12 months and of SHRs which developed or not developed heart failure at the age of 20-24 months. We measured cells dimensions, range and kinetics of electrically stimulated or initiated by caffeine contractions and Ca2+ transients, and investigated the response of cells to thapsigargin. RESULTS: The transversal dimensions of the LV myocytes of 6 months old SHRs showed approximately 20% increase with respect to transversal dimensions of their RV myocytes and LV and RV myocytes of WKY rats. The difference did not change with progressing age and in the heart failure. The LV myocytes of 6 or 12 months old SHRs showed slowed kinetics of the Ca2+ transients and of contraction and relaxation and decreased contractile response to 2 s superfusion with 15 mM caffeine preceded by 5 mM Ni2+ used as an index of the sarcoplasmic reticulum (SR) Ca2+ content. Despite of this the range of shortening and relative contribution of the SR to contraction (assessed by measuring of the residual contractile response to electrical stimulation in cells poisoned with thapsigargin) or relaxation (assessed by calculation of the ratio of rate constants of the electrically stimulated and stimulated by 30 s superfusion with caffeine Ca2+ transients) was not altered in the hypertrophied myocytes. Properties of the LV myocytes of the 20-24 old SHRs with or without heart failure did not differ from those of LV myocytes of younger SHRs. The contractile response to caffeine of their RV myocytes dropped to the level of that in the LV myocytes. CONCLUSION: Our results suggest that transition from the compensated hypertrophy to the heart failure in 20-24 months old SHRs did not result from the further changes in properties of the surviving myocytes. Data from literature suggest that myocyte apoptosis and remodeling of the extramyocyte space is the more likely reason. SN - 0867-5910 UR - https://www.unboundmedicine.com/medline/citation/10424720/Properties_of_ventricular_myocytes_isolated_from_the_hypertrophied_and_failing_hearts_of_spontaneously_hypertensive_rats_ L2 - https://antibodies.cancer.gov/detail/CPTC-SNCG-3 DB - PRIME DP - Unbound Medicine ER -