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Effect of sodium para-aminosalicylate on oxygen affinity in normal, sickle and fetal human blood.
J Pharmacol Exp Ther. 1976 Nov; 199(2):441-53.JP

Abstract

Sodium para-aminosalicylate (sodium salt of 2-hydroxy-4-aminobenzoic acid, Na-PAS) lowers the oxygen affinity of normal adult human placental, heterozygous and homozygous sickle cell anemic whole blood at 37 degrees C. The reduction of oxygen affinity is related to the type of hemoglobin in the blood. The mean P50 +/- S.E. at pH 7.40 for normal, placental, heterozygoud and homozygous sickle cell anemic blood in 26.2 +/- 0.1, 20.8 +/- 0.3, 26.8 +/- 0.3 and 31.0 +/- 0.5 mm Hg; in the presence of 5.7 mmol of Na-PAS per liter of blood the P50 values are increased to 28.0 +/- 0.3, 22.9 +/- 0.8, 30.5 +/- 0.6 and 33.9 +/- 0.3 mm Hg, respectively. The Bohr effect in normal and placental blood at this Na-PAS concentration is essentially unchanged: in heterozygous and homozygous sickle cell anemic blood, the Bohr factor (deta log P50/deta pH) is reduced from -0.48 +/- 0.02 to -0.41 +/- 0.01 and from -0.53 +/- 0.03 to -0.48 +/- 0.01. The Hill constants (n) of normal and placental blood are not affected by Na-PAS. In homozygous and heterozygous sickle blood, high concentrations of Na-PAS (22.9 mmol/l) decrease the Hill constant from 2.55 to 2.35 and from 2.56 to 2.28, respectively. Na-PAS is more firmly bound to red blood cells than to plasma. The binding of Na-PAS is probably primarily ionic in nature since the drug can be almost completely removed from blood components by dialysis. The changes in oxygen affinity caused by Na-PAS are consistent with conformational changes (R leads to T) which enhance the presence of deoxyhemoglobin.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10430

Citation

Laasberg, L H., et al. "Effect of Sodium Para-aminosalicylate On Oxygen Affinity in Normal, Sickle and Fetal Human Blood." The Journal of Pharmacology and Experimental Therapeutics, vol. 199, no. 2, 1976, pp. 441-53.
Laasberg LH, Hedley-Whyte J, Laver MB. Effect of sodium para-aminosalicylate on oxygen affinity in normal, sickle and fetal human blood. J Pharmacol Exp Ther. 1976;199(2):441-53.
Laasberg, L. H., Hedley-Whyte, J., & Laver, M. B. (1976). Effect of sodium para-aminosalicylate on oxygen affinity in normal, sickle and fetal human blood. The Journal of Pharmacology and Experimental Therapeutics, 199(2), 441-53.
Laasberg LH, Hedley-Whyte J, Laver MB. Effect of Sodium Para-aminosalicylate On Oxygen Affinity in Normal, Sickle and Fetal Human Blood. J Pharmacol Exp Ther. 1976;199(2):441-53. PubMed PMID: 10430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of sodium para-aminosalicylate on oxygen affinity in normal, sickle and fetal human blood. AU - Laasberg,L H, AU - Hedley-Whyte,J, AU - Laver,M B, PY - 1976/11/1/pubmed PY - 1976/11/1/medline PY - 1976/11/1/entrez SP - 441 EP - 53 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 199 IS - 2 N2 - Sodium para-aminosalicylate (sodium salt of 2-hydroxy-4-aminobenzoic acid, Na-PAS) lowers the oxygen affinity of normal adult human placental, heterozygous and homozygous sickle cell anemic whole blood at 37 degrees C. The reduction of oxygen affinity is related to the type of hemoglobin in the blood. The mean P50 +/- S.E. at pH 7.40 for normal, placental, heterozygoud and homozygous sickle cell anemic blood in 26.2 +/- 0.1, 20.8 +/- 0.3, 26.8 +/- 0.3 and 31.0 +/- 0.5 mm Hg; in the presence of 5.7 mmol of Na-PAS per liter of blood the P50 values are increased to 28.0 +/- 0.3, 22.9 +/- 0.8, 30.5 +/- 0.6 and 33.9 +/- 0.3 mm Hg, respectively. The Bohr effect in normal and placental blood at this Na-PAS concentration is essentially unchanged: in heterozygous and homozygous sickle cell anemic blood, the Bohr factor (deta log P50/deta pH) is reduced from -0.48 +/- 0.02 to -0.41 +/- 0.01 and from -0.53 +/- 0.03 to -0.48 +/- 0.01. The Hill constants (n) of normal and placental blood are not affected by Na-PAS. In homozygous and heterozygous sickle blood, high concentrations of Na-PAS (22.9 mmol/l) decrease the Hill constant from 2.55 to 2.35 and from 2.56 to 2.28, respectively. Na-PAS is more firmly bound to red blood cells than to plasma. The binding of Na-PAS is probably primarily ionic in nature since the drug can be almost completely removed from blood components by dialysis. The changes in oxygen affinity caused by Na-PAS are consistent with conformational changes (R leads to T) which enhance the presence of deoxyhemoglobin. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/10430/Effect_of_sodium_para_aminosalicylate_on_oxygen_affinity_in_normal_sickle_and_fetal_human_blood_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=10430 DB - PRIME DP - Unbound Medicine ER -