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Lipid mediators modulate the synthesis of interleukin 8 by human bone marrow stromal cells.
Cytokine. 1999 Aug; 11(8):606-10.C

Abstract

Bone marrow stromal cells regulate marrow haematopoiesis by secreting interleukins (IL) such as IL-8. Lipid mediators modulate IL-8 synthesis in numerous cell types. We have investigated the effects of 5 lipid mediators (PAF, PGE(2), LTB(4), 12-HETE and 15-HETE) on the spontaneous and cytokine-induced IL-8 synthesis by human bone marrow stromal cells. By using reverse-transcriptase polymerase chain reaction (RT-PCR) we demonstrate that these cells constitutively express IL-8 transcripts. By using a specific ELISA, we found that the production of IL-8 by marrow stromal cells is enhanced after stimulation with 12-HETE (1 microM) both in serum-free and serum-containing culture medium. LTB(4)(1 microM) enhances IL-8 production only in serum-supplemented medium. PAF, PGE(2)and 15-HETE (1 microM to 0.1 nM) have no effect on the spontaneous and serum-induced production of IL-8 by human bone marrow stromal cells. PGE(2)(1 microM or 10 nM) reduces marrow stromal cell IL-8 synthesis in response to IL-1alpha or TNF-alpha. In contrast, PAF, 12-HETE, 15-HETE and LTB(4)have no effect. In conclusion, various lipid mediators modulate the spontaneous, serum- or cytokine-induced IL-8 synthesis by bone marrow stromal cells, highlighting, for the first time, their potential role in the regulation of IL-8 production within the human bone marrow.

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Authors+Show Affiliations

Denizot Y
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue du Dr. Marcland, Limoges, 87025, France.
Godard A
No affiliation info available
Raher S
No affiliation info available
Trimoreau F
No affiliation info available
Praloran V
No affiliation info available

MeSH

12-Hydroxy-5,8,10,14-eicosatetraenoic AcidArachidonic AcidsBone Marrow CellsCells, CulturedDinoprostoneGene Expression RegulationHumansHydroxyeicosatetraenoic AcidsInterleukin-8Leukotriene B4Reverse Transcriptase Polymerase Chain ReactionStromal CellsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10433808

Citation

Denizot, Y, et al. "Lipid Mediators Modulate the Synthesis of Interleukin 8 By Human Bone Marrow Stromal Cells." Cytokine, vol. 11, no. 8, 1999, pp. 606-10.
Denizot Y, Godard A, Raher S, et al. Lipid mediators modulate the synthesis of interleukin 8 by human bone marrow stromal cells. Cytokine. 1999;11(8):606-10.
Denizot, Y., Godard, A., Raher, S., Trimoreau, F., & Praloran, V. (1999). Lipid mediators modulate the synthesis of interleukin 8 by human bone marrow stromal cells. Cytokine, 11(8), 606-10.
Denizot Y, et al. Lipid Mediators Modulate the Synthesis of Interleukin 8 By Human Bone Marrow Stromal Cells. Cytokine. 1999;11(8):606-10. PubMed PMID: 10433808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid mediators modulate the synthesis of interleukin 8 by human bone marrow stromal cells. AU - Denizot,Y, AU - Godard,A, AU - Raher,S, AU - Trimoreau,F, AU - Praloran,V, PY - 1999/8/6/pubmed PY - 1999/8/6/medline PY - 1999/8/6/entrez SP - 606 EP - 10 JF - Cytokine JO - Cytokine VL - 11 IS - 8 N2 - Bone marrow stromal cells regulate marrow haematopoiesis by secreting interleukins (IL) such as IL-8. Lipid mediators modulate IL-8 synthesis in numerous cell types. We have investigated the effects of 5 lipid mediators (PAF, PGE(2), LTB(4), 12-HETE and 15-HETE) on the spontaneous and cytokine-induced IL-8 synthesis by human bone marrow stromal cells. By using reverse-transcriptase polymerase chain reaction (RT-PCR) we demonstrate that these cells constitutively express IL-8 transcripts. By using a specific ELISA, we found that the production of IL-8 by marrow stromal cells is enhanced after stimulation with 12-HETE (1 microM) both in serum-free and serum-containing culture medium. LTB(4)(1 microM) enhances IL-8 production only in serum-supplemented medium. PAF, PGE(2)and 15-HETE (1 microM to 0.1 nM) have no effect on the spontaneous and serum-induced production of IL-8 by human bone marrow stromal cells. PGE(2)(1 microM or 10 nM) reduces marrow stromal cell IL-8 synthesis in response to IL-1alpha or TNF-alpha. In contrast, PAF, 12-HETE, 15-HETE and LTB(4)have no effect. In conclusion, various lipid mediators modulate the spontaneous, serum- or cytokine-induced IL-8 synthesis by bone marrow stromal cells, highlighting, for the first time, their potential role in the regulation of IL-8 production within the human bone marrow. SN - 1043-4666 UR - https://www.unboundmedicine.com/medline/citation/10433808/Lipid_mediators_modulate_the_synthesis_of_interleukin_8_by_human_bone_marrow_stromal_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(98)90467-7 DB - PRIME DP - Unbound Medicine ER -