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Antibodies to human recombinant tissue transglutaminase measured by radioligand assay: evidence for high diagnostic sensitivity for celiac disease.
Horm Metab Res 1999; 31(6):375-9HM

Abstract

Celiac disease is associated with endomysial antibodies (EmA), which have recently been reported to be directed to tissue transglutaminase (tTG). To demonstrate binding of antibodies to recombinant tTG, human tTG was cloned, expressed by in vitro transcription/translation and used to develop novel radioligand assays for combined and single detection of immunoglobulin A (IgA) and G (IgG)-specific antibodies. IgA and IgG-tTGA were found in 43 (95.6%) of 45 patients with newly-diagnosed celiac disease verified by biopsy. In addition, all 30 sera from patients with gastrointestinal symptoms and positive EmA were positive for IgA-tTGA, and all but one serum (96.7%) had antibodies of the IgG class. Receiver-operating characteristic analysis including 574 sera from healthy controls revealed a specificity of 99.5%. By means of these new assays, we identified all patients with endomysial antibodies and achieved, at equal specificity, an even improved sensitivity (95.6%) as compared to EmA (91.1%) detected by the standard immunofluorescence test. Here, we have provided direct evidence that recombinant tTG is a major target of antibodies in celiac disease. Our data suggest that tTGA measured by radioligand assay have the power to overcome the limitations of the EmA-test. This new strategy may considerably facilitate large-scale screening for silent and latent celiac disease.

Authors+Show Affiliations

Diabetes Research Institute, University of Duesseldorf, Germany. se@dfi.uni-duesseldorf.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10437627

Citation

Seissler, J, et al. "Antibodies to Human Recombinant Tissue Transglutaminase Measured By Radioligand Assay: Evidence for High Diagnostic Sensitivity for Celiac Disease." Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme, vol. 31, no. 6, 1999, pp. 375-9.
Seissler J, Boms S, Wohlrab U, et al. Antibodies to human recombinant tissue transglutaminase measured by radioligand assay: evidence for high diagnostic sensitivity for celiac disease. Horm Metab Res. 1999;31(6):375-9.
Seissler, J., Boms, S., Wohlrab, U., Morgenthaler, N. G., Mothes, T., Boehm, B. O., & Scherbaum, W. A. (1999). Antibodies to human recombinant tissue transglutaminase measured by radioligand assay: evidence for high diagnostic sensitivity for celiac disease. Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme, 31(6), pp. 375-9.
Seissler J, et al. Antibodies to Human Recombinant Tissue Transglutaminase Measured By Radioligand Assay: Evidence for High Diagnostic Sensitivity for Celiac Disease. Horm Metab Res. 1999;31(6):375-9. PubMed PMID: 10437627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibodies to human recombinant tissue transglutaminase measured by radioligand assay: evidence for high diagnostic sensitivity for celiac disease. AU - Seissler,J, AU - Boms,S, AU - Wohlrab,U, AU - Morgenthaler,N G, AU - Mothes,T, AU - Boehm,B O, AU - Scherbaum,W A, PY - 1999/8/7/pubmed PY - 1999/8/7/medline PY - 1999/8/7/entrez SP - 375 EP - 9 JF - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme JO - Horm. Metab. Res. VL - 31 IS - 6 N2 - Celiac disease is associated with endomysial antibodies (EmA), which have recently been reported to be directed to tissue transglutaminase (tTG). To demonstrate binding of antibodies to recombinant tTG, human tTG was cloned, expressed by in vitro transcription/translation and used to develop novel radioligand assays for combined and single detection of immunoglobulin A (IgA) and G (IgG)-specific antibodies. IgA and IgG-tTGA were found in 43 (95.6%) of 45 patients with newly-diagnosed celiac disease verified by biopsy. In addition, all 30 sera from patients with gastrointestinal symptoms and positive EmA were positive for IgA-tTGA, and all but one serum (96.7%) had antibodies of the IgG class. Receiver-operating characteristic analysis including 574 sera from healthy controls revealed a specificity of 99.5%. By means of these new assays, we identified all patients with endomysial antibodies and achieved, at equal specificity, an even improved sensitivity (95.6%) as compared to EmA (91.1%) detected by the standard immunofluorescence test. Here, we have provided direct evidence that recombinant tTG is a major target of antibodies in celiac disease. Our data suggest that tTGA measured by radioligand assay have the power to overcome the limitations of the EmA-test. This new strategy may considerably facilitate large-scale screening for silent and latent celiac disease. SN - 0018-5043 UR - https://www.unboundmedicine.com/medline/citation/10437627/Antibodies_to_human_recombinant_tissue_transglutaminase_measured_by_radioligand_assay:_evidence_for_high_diagnostic_sensitivity_for_celiac_disease_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2007-978758 DB - PRIME DP - Unbound Medicine ER -