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Plasma-mediated stimulation of neutrophil superoxide anion production during coronary artery bypass grafting: role of endothelin-1.
Thorac Cardiovasc Surg. 1999 Jun; 47(3):144-7.TC

Abstract

BACKGROUND

Activation of polymorphonuclear neutrophils (PMN) and subsequent release of free oxygen radicals, including the superoxide anion (O2-) has been shown to result in postischaemic myocardial dysfunction during coronary artery bypass grafting (CABG). Several neutrophil-oriented stimuli are known to be released from myocardium during ischaemia and reperfusion. Release of endothelin-1 has been documented during CABG. The aim of the current study was to evaluate plasma-mediated neutrophil stimulation and to verify whether endothelin-1, known to be a stimulus for PMN, is involved in plasma-mediated stimulation of PMN during coronary artery bypass grafting.

METHODS

Plasma samples from peripheral artery, peripheral vein, and coronary sinus were obtained from 21 patients undergoing CABG before aortic clamping (global ischaemia), immediately after beginning reperfusion, and 30 min after reperfusion as well as from healthy controls. Plasma was incubated with PMN isolated from healthy donors preincubated in the presence of saline or specific endothelin-1 receptor antagonist (ET-A). PMN O2- production was measured spectrophotometrically.

RESULTS

Plasma samples taken from the coronary sinus at the beginning of reperfusion were capable of higher stimulation of neutrophil superoxide anion production (24.2 +/- 2.0 nmol/5 x 10(6)PMN/30 min) than plasma obtained before reperfusion (15.6 +/- 1.5; p < 0.05) or plasma taken from peripheral artery (17.1 +/- 1.7; p < 0.05). Preincubation of PMN with endothelin-1 receptor antagonist decreased superoxide anion production by cells exposed to plasma taken from coronary sinus at the beginning of reperfusion (17.6 +/- 2.0, p < 0.05).

CONCLUSIONS

Transcardiac release of soluble stimuli for PMN occurs as a result of myocardial ischaemia during CABG. Endothelin-1 may be involved in the plasma-mediated stimulation of neutrophil superoxide anion production.

Authors+Show Affiliations

Department of Cardiosurgery, J. Strus Hospital, Poznań, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10443513

Citation

Bugajski, P, et al. "Plasma-mediated Stimulation of Neutrophil Superoxide Anion Production During Coronary Artery Bypass Grafting: Role of Endothelin-1." The Thoracic and Cardiovascular Surgeon, vol. 47, no. 3, 1999, pp. 144-7.
Bugajski P, Kalawski R, Baliński M, et al. Plasma-mediated stimulation of neutrophil superoxide anion production during coronary artery bypass grafting: role of endothelin-1. Thorac Cardiovasc Surg. 1999;47(3):144-7.
Bugajski, P., Kalawski, R., Baliński, M., Wysocki, H., Olszewski, R., Szczepanik, A., & Siminiak, T. (1999). Plasma-mediated stimulation of neutrophil superoxide anion production during coronary artery bypass grafting: role of endothelin-1. The Thoracic and Cardiovascular Surgeon, 47(3), 144-7.
Bugajski P, et al. Plasma-mediated Stimulation of Neutrophil Superoxide Anion Production During Coronary Artery Bypass Grafting: Role of Endothelin-1. Thorac Cardiovasc Surg. 1999;47(3):144-7. PubMed PMID: 10443513.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasma-mediated stimulation of neutrophil superoxide anion production during coronary artery bypass grafting: role of endothelin-1. AU - Bugajski,P, AU - Kalawski,R, AU - Baliński,M, AU - Wysocki,H, AU - Olszewski,R, AU - Szczepanik,A, AU - Siminiak,T, PY - 1999/8/12/pubmed PY - 1999/8/12/medline PY - 1999/8/12/entrez SP - 144 EP - 7 JF - The Thoracic and cardiovascular surgeon JO - Thorac Cardiovasc Surg VL - 47 IS - 3 N2 - BACKGROUND: Activation of polymorphonuclear neutrophils (PMN) and subsequent release of free oxygen radicals, including the superoxide anion (O2-) has been shown to result in postischaemic myocardial dysfunction during coronary artery bypass grafting (CABG). Several neutrophil-oriented stimuli are known to be released from myocardium during ischaemia and reperfusion. Release of endothelin-1 has been documented during CABG. The aim of the current study was to evaluate plasma-mediated neutrophil stimulation and to verify whether endothelin-1, known to be a stimulus for PMN, is involved in plasma-mediated stimulation of PMN during coronary artery bypass grafting. METHODS: Plasma samples from peripheral artery, peripheral vein, and coronary sinus were obtained from 21 patients undergoing CABG before aortic clamping (global ischaemia), immediately after beginning reperfusion, and 30 min after reperfusion as well as from healthy controls. Plasma was incubated with PMN isolated from healthy donors preincubated in the presence of saline or specific endothelin-1 receptor antagonist (ET-A). PMN O2- production was measured spectrophotometrically. RESULTS: Plasma samples taken from the coronary sinus at the beginning of reperfusion were capable of higher stimulation of neutrophil superoxide anion production (24.2 +/- 2.0 nmol/5 x 10(6)PMN/30 min) than plasma obtained before reperfusion (15.6 +/- 1.5; p < 0.05) or plasma taken from peripheral artery (17.1 +/- 1.7; p < 0.05). Preincubation of PMN with endothelin-1 receptor antagonist decreased superoxide anion production by cells exposed to plasma taken from coronary sinus at the beginning of reperfusion (17.6 +/- 2.0, p < 0.05). CONCLUSIONS: Transcardiac release of soluble stimuli for PMN occurs as a result of myocardial ischaemia during CABG. Endothelin-1 may be involved in the plasma-mediated stimulation of neutrophil superoxide anion production. SN - 0171-6425 UR - https://www.unboundmedicine.com/medline/citation/10443513/Plasma_mediated_stimulation_of_neutrophil_superoxide_anion_production_during_coronary_artery_bypass_grafting:_role_of_endothelin_1_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2007-1013129 DB - PRIME DP - Unbound Medicine ER -