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DNA typing of maternal HLA in congenital complete heart block: comparison with systemic lupus erythematosus and primary Sjögren's syndrome.
Arthritis Rheum. 1999 Aug; 42(8):1757-64.AR

Abstract

OBJECTIVE

To investigate which maternal HLA allele or haplotype is primarily associated with isolated congenital complete heart block (CCHB) in offspring.

METHODS

HLA class II typings were assessed by line probe assay and polymerase chain reaction-sequence-specific oligonucleotide probe methods, and HLA class I by the microlymphocytotoxicity test, in 13 Italian anti-Ro-positive mothers of children with CCHB and 41 anti-Ro-positive mothers with healthy children (20 mothers with systemic lupus erythematosus [SLE] and 21 with Sjögren's syndrome [SS]). Anti-Ro antibodies were studied by immunoblot.

RESULTS

HLA-DRB1*03011 and DRB1*03011; DQA1*0501;DQB1*0201 were more frequent in mothers of infants with CCHB than in mothers who had SLE, but not in mothers who had SS and whose children were healthy. Mothers of infants with CCHB were either HLA-B5/35, B17, or B44 positive and had a higher prevalence of B44;DRB11;DQA1*0501;DQB1*0301 and isolated anti-52-kd antibodies, which were absent in SS and SLE controls.

CONCLUSION

Mothers of infants with CCHB presented a strong genetic similarity to mothers who had SS, except for HLA class I phenotype. HLA-DRB1*03011;DQA1*0501;DQB1*0201 seemed not to be primary CCHB-associated genes, but were involved in an SS-like anti-Ro/La response. The combined presence of HLA-DRB1*03011 and anti-52-kd SSA/Ro antibodies conveyed the highest risk of giving birth to an affected child.

Authors+Show Affiliations

IRCCS Ospedale Maggiore, Università degli Studi, Milan, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10446878

Citation

Colombo, G, et al. "DNA Typing of Maternal HLA in Congenital Complete Heart Block: Comparison With Systemic Lupus Erythematosus and Primary Sjögren's Syndrome." Arthritis and Rheumatism, vol. 42, no. 8, 1999, pp. 1757-64.
Colombo G, Brucato A, Coluccio E, et al. DNA typing of maternal HLA in congenital complete heart block: comparison with systemic lupus erythematosus and primary Sjögren's syndrome. Arthritis Rheum. 1999;42(8):1757-64.
Colombo, G., Brucato, A., Coluccio, E., Compasso, S., Luzzana, C., Franceschini, F., Quinzanini, M., & Scorza, R. (1999). DNA typing of maternal HLA in congenital complete heart block: comparison with systemic lupus erythematosus and primary Sjögren's syndrome. Arthritis and Rheumatism, 42(8), 1757-64.
Colombo G, et al. DNA Typing of Maternal HLA in Congenital Complete Heart Block: Comparison With Systemic Lupus Erythematosus and Primary Sjögren's Syndrome. Arthritis Rheum. 1999;42(8):1757-64. PubMed PMID: 10446878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA typing of maternal HLA in congenital complete heart block: comparison with systemic lupus erythematosus and primary Sjögren's syndrome. AU - Colombo,G, AU - Brucato,A, AU - Coluccio,E, AU - Compasso,S, AU - Luzzana,C, AU - Franceschini,F, AU - Quinzanini,M, AU - Scorza,R, PY - 1999/8/14/pubmed PY - 1999/8/14/medline PY - 1999/8/14/entrez SP - 1757 EP - 64 JF - Arthritis and rheumatism JO - Arthritis Rheum. VL - 42 IS - 8 N2 - OBJECTIVE: To investigate which maternal HLA allele or haplotype is primarily associated with isolated congenital complete heart block (CCHB) in offspring. METHODS: HLA class II typings were assessed by line probe assay and polymerase chain reaction-sequence-specific oligonucleotide probe methods, and HLA class I by the microlymphocytotoxicity test, in 13 Italian anti-Ro-positive mothers of children with CCHB and 41 anti-Ro-positive mothers with healthy children (20 mothers with systemic lupus erythematosus [SLE] and 21 with Sjögren's syndrome [SS]). Anti-Ro antibodies were studied by immunoblot. RESULTS: HLA-DRB1*03011 and DRB1*03011; DQA1*0501;DQB1*0201 were more frequent in mothers of infants with CCHB than in mothers who had SLE, but not in mothers who had SS and whose children were healthy. Mothers of infants with CCHB were either HLA-B5/35, B17, or B44 positive and had a higher prevalence of B44;DRB11;DQA1*0501;DQB1*0301 and isolated anti-52-kd antibodies, which were absent in SS and SLE controls. CONCLUSION: Mothers of infants with CCHB presented a strong genetic similarity to mothers who had SS, except for HLA class I phenotype. HLA-DRB1*03011;DQA1*0501;DQB1*0201 seemed not to be primary CCHB-associated genes, but were involved in an SS-like anti-Ro/La response. The combined presence of HLA-DRB1*03011 and anti-52-kd SSA/Ro antibodies conveyed the highest risk of giving birth to an affected child. SN - 0004-3591 UR - https://www.unboundmedicine.com/medline/citation/10446878/DNA_typing_of_maternal_HLA_in_congenital_complete_heart_block:_comparison_with_systemic_lupus_erythematosus_and_primary_Sjögren's_syndrome_ L2 - https://doi.org/10.1002/1529-0131(199908)42:8<1757::AID-ANR27>3.0.CO;2-Z DB - PRIME DP - Unbound Medicine ER -