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Erythrocyte delta-aminolevulinic acid dehydratase genotype and other mechanisms affecting workers' susceptibility to lead.
J Occup Environ Med 1999; 41(8):662-8JO

Abstract

In this study, the role of delta-aminolevulinic acid dehydratase (ALAD) variants in lead susceptibility was examined. The study subjects comprised 223 male workers, and the relationship between their blood lead level and erythrocyte ALAD activity or plasma/urine delta-aminolevulinic acid level was studied. Leukocyte specimens from 11 workers, whose erythrocyte ALAD activities were as low as one-fifth that of the other normal workers, were subjected to analyses of their ALAD and ALAD alleles. Further, the entire exon fragment of the ALAD gene was analyzed by polymerase chain reaction, and the reaction product was used as a target for direct DNA sequencing. Genomic DNA analysis revealed that all 11 workers had the ALAD allele, whereas the entire ALAD gene analysis failed to indicate other variants, except for the Rsa I site. The depletion in erythrocyte ALAD activity was not found to be caused by the ALAD allele.

Authors+Show Affiliations

Department of Occupational and Environmental Health, Jichi Medical School, Tochigiken, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10457509

Citation

Nomiyama, K, et al. "Erythrocyte Delta-aminolevulinic Acid Dehydratase Genotype and Other Mechanisms Affecting Workers' Susceptibility to Lead." Journal of Occupational and Environmental Medicine, vol. 41, no. 8, 1999, pp. 662-8.
Nomiyama K, Nomiyama H, Xin KQ. Erythrocyte delta-aminolevulinic acid dehydratase genotype and other mechanisms affecting workers' susceptibility to lead. J Occup Environ Med. 1999;41(8):662-8.
Nomiyama, K., Nomiyama, H., & Xin, K. Q. (1999). Erythrocyte delta-aminolevulinic acid dehydratase genotype and other mechanisms affecting workers' susceptibility to lead. Journal of Occupational and Environmental Medicine, 41(8), pp. 662-8.
Nomiyama K, Nomiyama H, Xin KQ. Erythrocyte Delta-aminolevulinic Acid Dehydratase Genotype and Other Mechanisms Affecting Workers' Susceptibility to Lead. J Occup Environ Med. 1999;41(8):662-8. PubMed PMID: 10457509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Erythrocyte delta-aminolevulinic acid dehydratase genotype and other mechanisms affecting workers' susceptibility to lead. AU - Nomiyama,K, AU - Nomiyama,H, AU - Xin,K Q, PY - 1999/8/24/pubmed PY - 1999/8/24/medline PY - 1999/8/24/entrez SP - 662 EP - 8 JF - Journal of occupational and environmental medicine JO - J. Occup. Environ. Med. VL - 41 IS - 8 N2 - In this study, the role of delta-aminolevulinic acid dehydratase (ALAD) variants in lead susceptibility was examined. The study subjects comprised 223 male workers, and the relationship between their blood lead level and erythrocyte ALAD activity or plasma/urine delta-aminolevulinic acid level was studied. Leukocyte specimens from 11 workers, whose erythrocyte ALAD activities were as low as one-fifth that of the other normal workers, were subjected to analyses of their ALAD and ALAD alleles. Further, the entire exon fragment of the ALAD gene was analyzed by polymerase chain reaction, and the reaction product was used as a target for direct DNA sequencing. Genomic DNA analysis revealed that all 11 workers had the ALAD allele, whereas the entire ALAD gene analysis failed to indicate other variants, except for the Rsa I site. The depletion in erythrocyte ALAD activity was not found to be caused by the ALAD allele. SN - 1076-2752 UR - https://www.unboundmedicine.com/medline/citation/10457509/Erythrocyte_delta_aminolevulinic_acid_dehydratase_genotype_and_other_mechanisms_affecting_workers'_susceptibility_to_lead_ L2 - http://Insights.ovid.com/pubmed?pmid=10457509 DB - PRIME DP - Unbound Medicine ER -