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Adriamycin induces exocytosis in rat and beige mouse peritoneal mast cells: an ultrastructural, morphometric and biochemical study.
J Submicrosc Cytol Pathol. 1999 Apr; 31(2):279-86.JS

Abstract

The process of exocytosis was studied in rat and beige mouse peritoneal mast cells stimulated by adriamycin (ADR) at 37 degrees C and 22 degrees C. ADR induces a non cytotoxic histamine release that is followed by a significant uptake of the drug. Examination was performed by transmission electron microscopy and, at the same time, histamine release and ADR uptake were measured by spectrofluorimetry. ADR accumulation in mast cells was investigated also by fluorescence microscopy. In rat mast cells stimulated at 37 degrees C, the secretory process developed abruptly and was virtually complete after 30 sec. Electron microscopy showed rapid intracytoplasmic channel formation and extrusion of secretory granules; spectrofluorimetry revealed a massive release of histamine and rapid uptake of ADR. In addition, fluorescence microscopy showed mast cells exhibiting an intense orange-yellow fluorescent signal localized at the secretory granules. At 22 degrees C, rat mast cells showed alteration of the granules, cavity formation by fusion of the perigranular membrane and granule discharge due to fusion of the cavity membrane with the cell membrane. Histamine release and ADR uptake proceeded less quickly than at 37 degrees C. Quantitative analysis of rat mast cell ultrastructure demonstrated that histamine release induced by ADR stimulation was achieved by sequential exocytosis. This process presents both morphological and biochemical affinities with the exocytosis induced by basic secretagogues such as compound 48/80. In beige mouse mast cells the process of exocytosis progressed more slowly and was completed after 20 min at 37 degrees C. By electron microscopy, the cytoplasm presented a rigid structure due to abundance of actin-like fibrils. Granule fusion was an uncommon feature and exocytosis was mostly the result of single granule opening to the cell exterior without extrusion of granule matrices.

Authors+Show Affiliations

Department of Medical and Morphological Research, University of Udine, Italy. enrico.crivellato@kolbe.drmm.uniud.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10457614

Citation

Crivellato, E, et al. "Adriamycin Induces Exocytosis in Rat and Beige Mouse Peritoneal Mast Cells: an Ultrastructural, Morphometric and Biochemical Study." Journal of Submicroscopic Cytology and Pathology, vol. 31, no. 2, 1999, pp. 279-86.
Crivellato E, Candussio L, Decorti G, et al. Adriamycin induces exocytosis in rat and beige mouse peritoneal mast cells: an ultrastructural, morphometric and biochemical study. J Submicrosc Cytol Pathol. 1999;31(2):279-86.
Crivellato, E., Candussio, L., Decorti, G., Klugmann, F. B., & Mallardi, F. (1999). Adriamycin induces exocytosis in rat and beige mouse peritoneal mast cells: an ultrastructural, morphometric and biochemical study. Journal of Submicroscopic Cytology and Pathology, 31(2), 279-86.
Crivellato E, et al. Adriamycin Induces Exocytosis in Rat and Beige Mouse Peritoneal Mast Cells: an Ultrastructural, Morphometric and Biochemical Study. J Submicrosc Cytol Pathol. 1999;31(2):279-86. PubMed PMID: 10457614.
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TY - JOUR T1 - Adriamycin induces exocytosis in rat and beige mouse peritoneal mast cells: an ultrastructural, morphometric and biochemical study. AU - Crivellato,E, AU - Candussio,L, AU - Decorti,G, AU - Klugmann,F B, AU - Mallardi,F, PY - 1999/8/24/pubmed PY - 1999/8/24/medline PY - 1999/8/24/entrez SP - 279 EP - 86 JF - Journal of submicroscopic cytology and pathology JO - J Submicrosc Cytol Pathol VL - 31 IS - 2 N2 - The process of exocytosis was studied in rat and beige mouse peritoneal mast cells stimulated by adriamycin (ADR) at 37 degrees C and 22 degrees C. ADR induces a non cytotoxic histamine release that is followed by a significant uptake of the drug. Examination was performed by transmission electron microscopy and, at the same time, histamine release and ADR uptake were measured by spectrofluorimetry. ADR accumulation in mast cells was investigated also by fluorescence microscopy. In rat mast cells stimulated at 37 degrees C, the secretory process developed abruptly and was virtually complete after 30 sec. Electron microscopy showed rapid intracytoplasmic channel formation and extrusion of secretory granules; spectrofluorimetry revealed a massive release of histamine and rapid uptake of ADR. In addition, fluorescence microscopy showed mast cells exhibiting an intense orange-yellow fluorescent signal localized at the secretory granules. At 22 degrees C, rat mast cells showed alteration of the granules, cavity formation by fusion of the perigranular membrane and granule discharge due to fusion of the cavity membrane with the cell membrane. Histamine release and ADR uptake proceeded less quickly than at 37 degrees C. Quantitative analysis of rat mast cell ultrastructure demonstrated that histamine release induced by ADR stimulation was achieved by sequential exocytosis. This process presents both morphological and biochemical affinities with the exocytosis induced by basic secretagogues such as compound 48/80. In beige mouse mast cells the process of exocytosis progressed more slowly and was completed after 20 min at 37 degrees C. By electron microscopy, the cytoplasm presented a rigid structure due to abundance of actin-like fibrils. Granule fusion was an uncommon feature and exocytosis was mostly the result of single granule opening to the cell exterior without extrusion of granule matrices. SN - 1122-9497 UR - https://www.unboundmedicine.com/medline/citation/10457614/Adriamycin_induces_exocytosis_in_rat_and_beige_mouse_peritoneal_mast_cells:_an_ultrastructural_morphometric_and_biochemical_study_ L2 - https://antibodies.cancer.gov/detail/CPTC-AKR1B1-1 DB - PRIME DP - Unbound Medicine ER -