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Genetic susceptibility or resistance to autoimmune encephalomyelitis in MHC congenic mice is associated with differential production of pro- and anti-inflammatory cytokines.
Int Immunol. 1999 Sep; 11(9):1573-80.II

Abstract

Experimental allergic encephalomyelitis (EAE) is a T(h)1-type cell-mediated autoimmune disease induced by immunization with myelin proteins and mediated by CD4(+) T cells. Although susceptibility to EAE is dependent largely on MHC background, the B10.S strain is resistant to induction of EAE despite sharing the I-A(s) MHC locus with the susceptible SJL strain. Furthermore, NOD mice which spontaneously develop diabetes are susceptible to EAE induction with myelin oligodendrocyte glycoprotein (MOG) 35-55, whereas a MHC congenic strain, III, which also expresses I-A(g7) MHC haplotype does not develop diabetes and is also resistant to EAE induction. We induced EAE in these four strains of mice with MOG peptides 92-106 (for I-A(s) strains) and 35-55 (for I-A(g7) strains) in complete Freund's adjuvant. In the susceptible strains (SJL and NOD) in vitro, there are high levels of IFN-gamma production, whereas the resistant strains (B10.S or III) secreted primarily IL-4/IL-10 and transforming growth factor (TGF)-beta, and had decreased levels of IFN-gamma. When brains from susceptible and resistant mice were examined by immunohistochemical methods for cytokine expression, the brains from resistant mice showed fewer infiltrates which predominantly expressed IL-4 and IL-10 and/or TGF-beta. Brains from NOD and SJL with EAE showed mainly IL-2 and IFN-gamma positive cells. Thus, resistance to MOG induced EAE in B10.S and III mouse strains is related to non-MHC genes and is associated with an altered balance of pro- and anti-inflammatory cytokines both in lymphoid tissue and in the brain following immunization with myelin antigens.

Authors+Show Affiliations

Center for Neurologic Diseases, Brigham and Women's Hospital, 77 Avenue Louis Pasteur, HIM 730, Boston, MA 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10464178

Citation

Maron, R, et al. "Genetic Susceptibility or Resistance to Autoimmune Encephalomyelitis in MHC Congenic Mice Is Associated With Differential Production of Pro- and Anti-inflammatory Cytokines." International Immunology, vol. 11, no. 9, 1999, pp. 1573-80.
Maron R, Hancock WW, Slavin A, et al. Genetic susceptibility or resistance to autoimmune encephalomyelitis in MHC congenic mice is associated with differential production of pro- and anti-inflammatory cytokines. Int Immunol. 1999;11(9):1573-80.
Maron, R., Hancock, W. W., Slavin, A., Hattori, M., Kuchroo, V., & Weiner, H. L. (1999). Genetic susceptibility or resistance to autoimmune encephalomyelitis in MHC congenic mice is associated with differential production of pro- and anti-inflammatory cytokines. International Immunology, 11(9), 1573-80.
Maron R, et al. Genetic Susceptibility or Resistance to Autoimmune Encephalomyelitis in MHC Congenic Mice Is Associated With Differential Production of Pro- and Anti-inflammatory Cytokines. Int Immunol. 1999;11(9):1573-80. PubMed PMID: 10464178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic susceptibility or resistance to autoimmune encephalomyelitis in MHC congenic mice is associated with differential production of pro- and anti-inflammatory cytokines. AU - Maron,R, AU - Hancock,W W, AU - Slavin,A, AU - Hattori,M, AU - Kuchroo,V, AU - Weiner,H L, PY - 1999/8/28/pubmed PY - 1999/8/28/medline PY - 1999/8/28/entrez SP - 1573 EP - 80 JF - International immunology JO - Int. Immunol. VL - 11 IS - 9 N2 - Experimental allergic encephalomyelitis (EAE) is a T(h)1-type cell-mediated autoimmune disease induced by immunization with myelin proteins and mediated by CD4(+) T cells. Although susceptibility to EAE is dependent largely on MHC background, the B10.S strain is resistant to induction of EAE despite sharing the I-A(s) MHC locus with the susceptible SJL strain. Furthermore, NOD mice which spontaneously develop diabetes are susceptible to EAE induction with myelin oligodendrocyte glycoprotein (MOG) 35-55, whereas a MHC congenic strain, III, which also expresses I-A(g7) MHC haplotype does not develop diabetes and is also resistant to EAE induction. We induced EAE in these four strains of mice with MOG peptides 92-106 (for I-A(s) strains) and 35-55 (for I-A(g7) strains) in complete Freund's adjuvant. In the susceptible strains (SJL and NOD) in vitro, there are high levels of IFN-gamma production, whereas the resistant strains (B10.S or III) secreted primarily IL-4/IL-10 and transforming growth factor (TGF)-beta, and had decreased levels of IFN-gamma. When brains from susceptible and resistant mice were examined by immunohistochemical methods for cytokine expression, the brains from resistant mice showed fewer infiltrates which predominantly expressed IL-4 and IL-10 and/or TGF-beta. Brains from NOD and SJL with EAE showed mainly IL-2 and IFN-gamma positive cells. Thus, resistance to MOG induced EAE in B10.S and III mouse strains is related to non-MHC genes and is associated with an altered balance of pro- and anti-inflammatory cytokines both in lymphoid tissue and in the brain following immunization with myelin antigens. SN - 0953-8178 UR - https://www.unboundmedicine.com/medline/citation/10464178/Genetic_susceptibility_or_resistance_to_autoimmune_encephalomyelitis_in_MHC_congenic_mice_is_associated_with_differential_production_of_pro__and_anti_inflammatory_cytokines_ L2 - https://academic.oup.com/intimm/article-lookup/doi/10.1093/intimm/11.9.1573 DB - PRIME DP - Unbound Medicine ER -