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The frequency of conjugative transposition of Tn916 is not determined by the frequency of excision.
J Bacteriol 1999; 181(17):5414-8JB

Abstract

Excision and formation of a covalently closed circular transposon molecule are required for conjugative transposition of Tn916 but are not the only factors that limit the frequency of conjugative transposition from one host to another. We found that in gram-positive bacteria, an increase in the frequency of excision and circularization of Tn916 caused by expression of integrase (Int) and excisionase (Xis) from a xylose-inducible promoter does not lead to an increase in the frequency of conjugative transposition. We also found that the concentration of Int and Xis in the recipient cell does not limit the frequency of conjugative transposition and that increased excision does not result in increased expression of transfer functions required to mobilize a plasmid containing the Tn916 origin of transfer. We conclude that in gram-positive hosts in which the Tn916 functions Int and Xis are overexpressed, the frequency of conjugative transposition is limited by the availability of transfer functions.

Authors+Show Affiliations

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10464215

Citation

Marra, D, et al. "The Frequency of Conjugative Transposition of Tn916 Is Not Determined By the Frequency of Excision." Journal of Bacteriology, vol. 181, no. 17, 1999, pp. 5414-8.
Marra D, Pethel B, Churchward GG, et al. The frequency of conjugative transposition of Tn916 is not determined by the frequency of excision. J Bacteriol. 1999;181(17):5414-8.
Marra, D., Pethel, B., Churchward, G. G., & Scott, J. R. (1999). The frequency of conjugative transposition of Tn916 is not determined by the frequency of excision. Journal of Bacteriology, 181(17), pp. 5414-8.
Marra D, et al. The Frequency of Conjugative Transposition of Tn916 Is Not Determined By the Frequency of Excision. J Bacteriol. 1999;181(17):5414-8. PubMed PMID: 10464215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The frequency of conjugative transposition of Tn916 is not determined by the frequency of excision. AU - Marra,D, AU - Pethel,B, AU - Churchward,G G, AU - Scott,J R, PY - 1999/8/28/pubmed PY - 1999/8/28/medline PY - 1999/8/28/entrez SP - 5414 EP - 8 JF - Journal of bacteriology JO - J. Bacteriol. VL - 181 IS - 17 N2 - Excision and formation of a covalently closed circular transposon molecule are required for conjugative transposition of Tn916 but are not the only factors that limit the frequency of conjugative transposition from one host to another. We found that in gram-positive bacteria, an increase in the frequency of excision and circularization of Tn916 caused by expression of integrase (Int) and excisionase (Xis) from a xylose-inducible promoter does not lead to an increase in the frequency of conjugative transposition. We also found that the concentration of Int and Xis in the recipient cell does not limit the frequency of conjugative transposition and that increased excision does not result in increased expression of transfer functions required to mobilize a plasmid containing the Tn916 origin of transfer. We conclude that in gram-positive hosts in which the Tn916 functions Int and Xis are overexpressed, the frequency of conjugative transposition is limited by the availability of transfer functions. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/10464215/The_frequency_of_conjugative_transposition_of_Tn916_is_not_determined_by_the_frequency_of_excision_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=10464215 DB - PRIME DP - Unbound Medicine ER -