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Expression of T-type Ca(2+) channels in ventricular cells from hypertrophied rat hearts.
J Mol Cell Cardiol. 1999 Sep; 31(9):1617-25.JM

Abstract

In this study we examined the existence of T-type Ca(2+) current in ventricular myocytes isolated from rats with pressure-overload hypertrophy. The whole-cell clamp technique was used to record Ca(2+) currents in enzymatically dissociated ventricular cells. T- and L-type Ca(2+) currents were separated by applying voltage steps to different test potentials from a holding potential of -80 mV and -50 mV. T-type Ca(2+) current was defined as the difference between the currents from the two holding potentials. Ventricular myocytes from sham-operated rats showed only L-type Ca(2+) current (maximal density -13.9+/-1.3 pA/pF n=17), whereas ventricular myocytes isolated from rats with aortic stenosis showed both L- and T-type Ca(2+) currents. The average values of T- and L-type Ca(2+) current density were -4.8+/-0.4 pA/pF and -12.4+/-0.9 pA/pF (n=32), respectively. T-type Ca(2+) current was distinguished from L-type Ca(2+) current by its voltage dependence, its kinetics and by its strong blockade by nickel 50 microM. In conclusion, we have demonstrated that hypertrophied ventricular rat cells express T-type Ca(2+) channels and this finding strongly supports a role for this channel in regulating growth processes in cardiac tissue.

Authors+Show Affiliations

Institute of Pharmacology and Toxicology (CSIC-UCM), Universidad Complutense, Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10471346

Citation

Martínez, M L., et al. "Expression of T-type Ca(2+) Channels in Ventricular Cells From Hypertrophied Rat Hearts." Journal of Molecular and Cellular Cardiology, vol. 31, no. 9, 1999, pp. 1617-25.
Martínez ML, Heredia MP, Delgado C. Expression of T-type Ca(2+) channels in ventricular cells from hypertrophied rat hearts. J Mol Cell Cardiol. 1999;31(9):1617-25.
Martínez, M. L., Heredia, M. P., & Delgado, C. (1999). Expression of T-type Ca(2+) channels in ventricular cells from hypertrophied rat hearts. Journal of Molecular and Cellular Cardiology, 31(9), 1617-25.
Martínez ML, Heredia MP, Delgado C. Expression of T-type Ca(2+) Channels in Ventricular Cells From Hypertrophied Rat Hearts. J Mol Cell Cardiol. 1999;31(9):1617-25. PubMed PMID: 10471346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of T-type Ca(2+) channels in ventricular cells from hypertrophied rat hearts. AU - Martínez,M L, AU - Heredia,M P, AU - Delgado,C, PY - 1999/9/2/pubmed PY - 1999/9/2/medline PY - 1999/9/2/entrez SP - 1617 EP - 25 JF - Journal of molecular and cellular cardiology JO - J Mol Cell Cardiol VL - 31 IS - 9 N2 - In this study we examined the existence of T-type Ca(2+) current in ventricular myocytes isolated from rats with pressure-overload hypertrophy. The whole-cell clamp technique was used to record Ca(2+) currents in enzymatically dissociated ventricular cells. T- and L-type Ca(2+) currents were separated by applying voltage steps to different test potentials from a holding potential of -80 mV and -50 mV. T-type Ca(2+) current was defined as the difference between the currents from the two holding potentials. Ventricular myocytes from sham-operated rats showed only L-type Ca(2+) current (maximal density -13.9+/-1.3 pA/pF n=17), whereas ventricular myocytes isolated from rats with aortic stenosis showed both L- and T-type Ca(2+) currents. The average values of T- and L-type Ca(2+) current density were -4.8+/-0.4 pA/pF and -12.4+/-0.9 pA/pF (n=32), respectively. T-type Ca(2+) current was distinguished from L-type Ca(2+) current by its voltage dependence, its kinetics and by its strong blockade by nickel 50 microM. In conclusion, we have demonstrated that hypertrophied ventricular rat cells express T-type Ca(2+) channels and this finding strongly supports a role for this channel in regulating growth processes in cardiac tissue. SN - 0022-2828 UR - https://www.unboundmedicine.com/medline/citation/10471346/Expression_of_T_type_Ca_2+__channels_in_ventricular_cells_from_hypertrophied_rat_hearts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022282899909987 DB - PRIME DP - Unbound Medicine ER -