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Polarized type 1 cytokine profile in bronchoalveolar lavage T cells of patients with hypersensitivity pneumonitis.
J Immunol. 1999 Sep 15; 163(6):3516-23.JI

Abstract

Hypersensitivity pneumonitis (HP) is characterized by an inflammatory lymphocytic alveolitis comprised of both CD8+ and CD4+ T cells. Animal models suggest that HP is facilitated by overproduction of IFN-gamma, and that IL-10 ameliorates severity of the disease, indicating a Th1-type response. To determine whether a Th1 phenotype in HP also exists clinically, bronchoalveolar lavage (BAL) and peripheral blood (PB) T cells were obtained from HP individuals and analyzed for Th1 vs Th2 cytokine profiles. It was determined that soluble OKT3-stimulated BAL T cells cocultured with alveolar macrophages produced more IFN-gamma and less IL-10 than PB T cells cocultured with monocytes, but no difference was observed in IL-4 production. The monocytic cells did not account for this difference, as CD80 and CD86 expressions were similar, and coculturing PB T cells with alveolar macrophages resulted in no difference in IFN-gamma production. Similarly, there was no difference in IL-12 production between stimulated BAL or PB T cells; however, addition of rIL-12 significantly increased production of IFN-gamma by BAL T cells, but not by PB T cells. This effect was due to a difference in IL-12R expression. High affinity IL-12R were only present in association with BAL T cells. These studies indicate that clinical HP is characterized by a predominance of IFN-gamma-producing T cells, perhaps resulting from a reduction in IL-10 production and an increase in high affinity IL-12R compared with blood T cells.

Authors+Show Affiliations

First Department of Internal Medicine, Kumamoto University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10477626

Citation

Yamasaki, H, et al. "Polarized Type 1 Cytokine Profile in Bronchoalveolar Lavage T Cells of Patients With Hypersensitivity Pneumonitis." Journal of Immunology (Baltimore, Md. : 1950), vol. 163, no. 6, 1999, pp. 3516-23.
Yamasaki H, Ando M, Brazer W, et al. Polarized type 1 cytokine profile in bronchoalveolar lavage T cells of patients with hypersensitivity pneumonitis. J Immunol. 1999;163(6):3516-23.
Yamasaki, H., Ando, M., Brazer, W., Center, D. M., & Cruikshank, W. W. (1999). Polarized type 1 cytokine profile in bronchoalveolar lavage T cells of patients with hypersensitivity pneumonitis. Journal of Immunology (Baltimore, Md. : 1950), 163(6), 3516-23.
Yamasaki H, et al. Polarized Type 1 Cytokine Profile in Bronchoalveolar Lavage T Cells of Patients With Hypersensitivity Pneumonitis. J Immunol. 1999 Sep 15;163(6):3516-23. PubMed PMID: 10477626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polarized type 1 cytokine profile in bronchoalveolar lavage T cells of patients with hypersensitivity pneumonitis. AU - Yamasaki,H, AU - Ando,M, AU - Brazer,W, AU - Center,D M, AU - Cruikshank,W W, PY - 1999/9/8/pubmed PY - 1999/9/8/medline PY - 1999/9/8/entrez SP - 3516 EP - 23 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 163 IS - 6 N2 - Hypersensitivity pneumonitis (HP) is characterized by an inflammatory lymphocytic alveolitis comprised of both CD8+ and CD4+ T cells. Animal models suggest that HP is facilitated by overproduction of IFN-gamma, and that IL-10 ameliorates severity of the disease, indicating a Th1-type response. To determine whether a Th1 phenotype in HP also exists clinically, bronchoalveolar lavage (BAL) and peripheral blood (PB) T cells were obtained from HP individuals and analyzed for Th1 vs Th2 cytokine profiles. It was determined that soluble OKT3-stimulated BAL T cells cocultured with alveolar macrophages produced more IFN-gamma and less IL-10 than PB T cells cocultured with monocytes, but no difference was observed in IL-4 production. The monocytic cells did not account for this difference, as CD80 and CD86 expressions were similar, and coculturing PB T cells with alveolar macrophages resulted in no difference in IFN-gamma production. Similarly, there was no difference in IL-12 production between stimulated BAL or PB T cells; however, addition of rIL-12 significantly increased production of IFN-gamma by BAL T cells, but not by PB T cells. This effect was due to a difference in IL-12R expression. High affinity IL-12R were only present in association with BAL T cells. These studies indicate that clinical HP is characterized by a predominance of IFN-gamma-producing T cells, perhaps resulting from a reduction in IL-10 production and an increase in high affinity IL-12R compared with blood T cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/10477626/Polarized_type_1_cytokine_profile_in_bronchoalveolar_lavage_T_cells_of_patients_with_hypersensitivity_pneumonitis_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=10477626 DB - PRIME DP - Unbound Medicine ER -