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Elevated production of salivary nitric oxide in oral mucosal diseases.
J Oral Pathol Med. 1999 Sep; 28(8):355-9.JO

Abstract

Nitric oxide (NO) is known to play an important role in biological systems. In this study, we measured levels of NO in the saliva of 39 patients with oral mucosal diseases: 21 had oral lichen planus (OLP) and 18 had recurrent aphthous ulceration (RAU). NO was assayed using the Griess reagent, which measures nitrite (NO2), the byproduct of NO. NO2 was detected in all tested samples, and levels in the saliva of patients were significantly increased relative to those of healthy subjects. We also examined the effect of NO on fibroblasts, keratinocytes and NA cells (an epithelial cancer cell line) in vitro. S-nitroso-N-acetyl-DL-penicillamine (SNAP) and 3-morpholinosydnonimine (SIN-1) were used as NO donating reagents. The results revealed that cell viability was significantly reduced by NO derived from SNAP and SIN-1 in a dose-dependent manner. Although the role of salivary NO in normal physiology is as yet unknown, these findings suggest that excessive salivary NO plays a potential role in modifying oral mucosal diseases as a physiopathological regulator.

Authors+Show Affiliations

Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10478960

Citation

Ohashi, M, et al. "Elevated Production of Salivary Nitric Oxide in Oral Mucosal Diseases." Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, vol. 28, no. 8, 1999, pp. 355-9.
Ohashi M, Iwase M, Nagumo M. Elevated production of salivary nitric oxide in oral mucosal diseases. J Oral Pathol Med. 1999;28(8):355-9.
Ohashi, M., Iwase, M., & Nagumo, M. (1999). Elevated production of salivary nitric oxide in oral mucosal diseases. Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 28(8), 355-9.
Ohashi M, Iwase M, Nagumo M. Elevated Production of Salivary Nitric Oxide in Oral Mucosal Diseases. J Oral Pathol Med. 1999;28(8):355-9. PubMed PMID: 10478960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated production of salivary nitric oxide in oral mucosal diseases. AU - Ohashi,M, AU - Iwase,M, AU - Nagumo,M, PY - 1999/9/9/pubmed PY - 1999/9/9/medline PY - 1999/9/9/entrez SP - 355 EP - 9 JF - Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology JO - J Oral Pathol Med VL - 28 IS - 8 N2 - Nitric oxide (NO) is known to play an important role in biological systems. In this study, we measured levels of NO in the saliva of 39 patients with oral mucosal diseases: 21 had oral lichen planus (OLP) and 18 had recurrent aphthous ulceration (RAU). NO was assayed using the Griess reagent, which measures nitrite (NO2), the byproduct of NO. NO2 was detected in all tested samples, and levels in the saliva of patients were significantly increased relative to those of healthy subjects. We also examined the effect of NO on fibroblasts, keratinocytes and NA cells (an epithelial cancer cell line) in vitro. S-nitroso-N-acetyl-DL-penicillamine (SNAP) and 3-morpholinosydnonimine (SIN-1) were used as NO donating reagents. The results revealed that cell viability was significantly reduced by NO derived from SNAP and SIN-1 in a dose-dependent manner. Although the role of salivary NO in normal physiology is as yet unknown, these findings suggest that excessive salivary NO plays a potential role in modifying oral mucosal diseases as a physiopathological regulator. SN - 0904-2512 UR - https://www.unboundmedicine.com/medline/citation/10478960/Elevated_production_of_salivary_nitric_oxide_in_oral_mucosal_diseases_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0904-2512&date=1999&volume=28&issue=8&spage=355 DB - PRIME DP - Unbound Medicine ER -