Tags

Type your tag names separated by a space and hit enter

Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro.
J Med Microbiol. 1999 Sep; 48(9):801-810.JM

Abstract

Ten recent clinical isolates of Salmonella serotype Typhimurium from man that were tested for their invasiveness in rabbit ileal explants in vitro, were compared with Typhimurium strain TML, a well-characterised invasive strain isolated from a case of human gastro-enteritis. Nine of the 10 strains showed invasiveness that was comparable to that of strain TML. One isolate (GM3) was apparently substantially less invasive; electron microscopy showed this strain to be histotoxic - the probable reason for its reduced recovery from ileal mucosa and thus apparent 'low' invasiveness. Salmonella serotype Choleraesuis strain A50, isolated from a case of systemic salmonellosis in pigs, and serotype Dublin strain 3246, isolated from a case of systemic salmonellosis in calves, were also examined. Dublin strain 3246, when grown at 37 degrees C and used immediately in the invasion assay, damaged the mucosa in a manner similar to that of Typhimurium strain GM3, whereas Dublin strain 3246 grown at 37 degrees C and stored overnight at 4 degrees C did not. This was reflected in an apparently lower invasiveness of freshly grown organisms compared with that of organisms stored at 4 degrees C. In contrast, the histotoxicity of Typhimurium strain GM3 was not affected by storage at 4 degrees C. When stored at 4 degrees C, the levels of invasiveness of Choleraesuis strain A50 and Dublin strain 3246 were not significantly different from each other or from Typhimurium strain TML.

Authors+Show Affiliations

Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences.Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences.Department of Physiology, Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT.Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN.Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10482290

Citation

Bolton, Alex J., et al. "Invasiveness of Salmonella Serotypes Typhimurium, Choleraesuis and Dublin for Rabbit Terminal Ileum in Vitro." Journal of Medical Microbiology, vol. 48, no. 9, 1999, pp. 801-810.
Bolton AJ, Martin GD, Osborne MP, et al. Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro. J Med Microbiol. 1999;48(9):801-810.
Bolton, A. J., Martin, G. D., Osborne, M. P., Wallis, T. S., & Stephen, J. (1999). Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro. Journal of Medical Microbiology, 48(9), 801-810. https://doi.org/10.1099/00222615-48-9-801
Bolton AJ, et al. Invasiveness of Salmonella Serotypes Typhimurium, Choleraesuis and Dublin for Rabbit Terminal Ileum in Vitro. J Med Microbiol. 1999;48(9):801-810. PubMed PMID: 10482290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro. AU - Bolton,Alex J, AU - Martin,GIllian D, AU - Osborne,Michael P, AU - Wallis,Tim S, AU - Stephen,John, PY - 1999/9/11/pubmed PY - 1999/9/11/medline PY - 1999/9/11/entrez SP - 801 EP - 810 JF - Journal of medical microbiology JO - J Med Microbiol VL - 48 IS - 9 N2 - Ten recent clinical isolates of Salmonella serotype Typhimurium from man that were tested for their invasiveness in rabbit ileal explants in vitro, were compared with Typhimurium strain TML, a well-characterised invasive strain isolated from a case of human gastro-enteritis. Nine of the 10 strains showed invasiveness that was comparable to that of strain TML. One isolate (GM3) was apparently substantially less invasive; electron microscopy showed this strain to be histotoxic - the probable reason for its reduced recovery from ileal mucosa and thus apparent 'low' invasiveness. Salmonella serotype Choleraesuis strain A50, isolated from a case of systemic salmonellosis in pigs, and serotype Dublin strain 3246, isolated from a case of systemic salmonellosis in calves, were also examined. Dublin strain 3246, when grown at 37 degrees C and used immediately in the invasion assay, damaged the mucosa in a manner similar to that of Typhimurium strain GM3, whereas Dublin strain 3246 grown at 37 degrees C and stored overnight at 4 degrees C did not. This was reflected in an apparently lower invasiveness of freshly grown organisms compared with that of organisms stored at 4 degrees C. In contrast, the histotoxicity of Typhimurium strain GM3 was not affected by storage at 4 degrees C. When stored at 4 degrees C, the levels of invasiveness of Choleraesuis strain A50 and Dublin strain 3246 were not significantly different from each other or from Typhimurium strain TML. SN - 0022-2615 UR - https://www.unboundmedicine.com/medline/citation/10482290/Invasiveness_of_Salmonella_serotypes_Typhimurium_Choleraesuis_and_Dublin_for_rabbit_terminal_ileum_in_vitro_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/00222615-48-9-801 DB - PRIME DP - Unbound Medicine ER -