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Diagnosis of pancreatic cancer by detecting telomerase activity in pancreatic juice: comparison with K-ras mutations.
Am J Gastroenterol. 1999 Sep; 94(9):2513-8.AJ

Abstract

OBJECTIVE

Telomerase activity is reported to be specific and very frequent in human malignancy. K-ras mutations are also very frequently detected in pancreatic cancer, but their specificity for pancreatic cancer is controversial. We examined the telomerase activity and K-ras mutations in pancreatic juice from patients with pancreatic disease.

METHODS

Pancreatic juice was obtained endoscopically at endoscopic retrograde pancreatography from 10 patients with pancreatic cancer, three with chronic pancreatitis, and three with a normal pancreas. The telomerase activity in pancreatic juice was assayed by telomeric repeat amplification protocol. K-ras mutations in exon 1 codon 12 were examined by the two-step polymerase chain reaction combined with restriction enzyme digestion, followed by single-strand conformation polymorphism analysis and direct sequencing.

RESULTS

Telomerase activity of >5.0 was detected in eight of 10 (80%) subjects with pancreatic cancer, but in none with chronic pancreatitis or normal pancreas. K-ras mutations were detected not only in eight of 10 (80%) subjects with pancreatic cancer but also in two of three with chronic pancreatitis and in one of three with a normal pancreas.

CONCLUSIONS

It was shown that the detection of telomerase activity in pancreatic juice is a more useful diagnostic tool for pancreatic cancer than that of K-ras mutations.

Authors+Show Affiliations

Department of Gastroenterology, Osaka Medical Center for Cancer and Cardiovascular Disease, Higashinari, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10484017

Citation

Uehara, H, et al. "Diagnosis of Pancreatic Cancer By Detecting Telomerase Activity in Pancreatic Juice: Comparison With K-ras Mutations." The American Journal of Gastroenterology, vol. 94, no. 9, 1999, pp. 2513-8.
Uehara H, Nakaizumi A, Tatsuta M, et al. Diagnosis of pancreatic cancer by detecting telomerase activity in pancreatic juice: comparison with K-ras mutations. Am J Gastroenterol. 1999;94(9):2513-8.
Uehara, H., Nakaizumi, A., Tatsuta, M., Baba, M., Takenaka, A., Uedo, N., Sakai, N., Yano, H., Iishi, H., Ohigashi, H., Ishikawa, O., Okada, S., & Kakizoe, T. (1999). Diagnosis of pancreatic cancer by detecting telomerase activity in pancreatic juice: comparison with K-ras mutations. The American Journal of Gastroenterology, 94(9), 2513-8.
Uehara H, et al. Diagnosis of Pancreatic Cancer By Detecting Telomerase Activity in Pancreatic Juice: Comparison With K-ras Mutations. Am J Gastroenterol. 1999;94(9):2513-8. PubMed PMID: 10484017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of pancreatic cancer by detecting telomerase activity in pancreatic juice: comparison with K-ras mutations. AU - Uehara,H, AU - Nakaizumi,A, AU - Tatsuta,M, AU - Baba,M, AU - Takenaka,A, AU - Uedo,N, AU - Sakai,N, AU - Yano,H, AU - Iishi,H, AU - Ohigashi,H, AU - Ishikawa,O, AU - Okada,S, AU - Kakizoe,T, PY - 1999/9/14/pubmed PY - 1999/9/14/medline PY - 1999/9/14/entrez SP - 2513 EP - 8 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 94 IS - 9 N2 - OBJECTIVE: Telomerase activity is reported to be specific and very frequent in human malignancy. K-ras mutations are also very frequently detected in pancreatic cancer, but their specificity for pancreatic cancer is controversial. We examined the telomerase activity and K-ras mutations in pancreatic juice from patients with pancreatic disease. METHODS: Pancreatic juice was obtained endoscopically at endoscopic retrograde pancreatography from 10 patients with pancreatic cancer, three with chronic pancreatitis, and three with a normal pancreas. The telomerase activity in pancreatic juice was assayed by telomeric repeat amplification protocol. K-ras mutations in exon 1 codon 12 were examined by the two-step polymerase chain reaction combined with restriction enzyme digestion, followed by single-strand conformation polymorphism analysis and direct sequencing. RESULTS: Telomerase activity of >5.0 was detected in eight of 10 (80%) subjects with pancreatic cancer, but in none with chronic pancreatitis or normal pancreas. K-ras mutations were detected not only in eight of 10 (80%) subjects with pancreatic cancer but also in two of three with chronic pancreatitis and in one of three with a normal pancreas. CONCLUSIONS: It was shown that the detection of telomerase activity in pancreatic juice is a more useful diagnostic tool for pancreatic cancer than that of K-ras mutations. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/10484017/Diagnosis_of_pancreatic_cancer_by_detecting_telomerase_activity_in_pancreatic_juice:_comparison_with_K_ras_mutations_ L2 - http://Insights.ovid.com/pubmed?pmid=10484017 DB - PRIME DP - Unbound Medicine ER -