Tags

Type your tag names separated by a space and hit enter

Endocytosis and vacuolar morphology in Saccharomyces cerevisiae are altered in response to ethanol stress or heat shock.
Yeast. 1999 Sep 15; 15(12):1211-22.Y

Abstract

The vital lipophilic dye N-(3-triethylammoniumpropyl)-4-[6-(4-(diethylamino)phenyl]hexatrie nyl) pyridinium dibromide (FM 4-64) was used to study the effect of ethanol stress and heat shock on endocytosis in the yeast Saccharomyces cerevisiae. Yeast cells stained with FM 4-64 were placed in a culture chamber and the internalization of the dye was monitored by fluorescence microscopy during perfusion of the cells with fresh growth medium. In the absence of ethanol in the perfusion medium, the internalization of FM 4-64 from the plasma membrane to the vacuolar membrane by yeast cells harvested from the exponential phase of growth was completed in 30 min. The presence of 6% (v/v) ethanol in the perfusion medium had no obvious effect on the internalization of FM 4-64 from the plasma membrane, but did lead to an accumulation of the dye in endocytic intermediates. Consequently, vacuolar membrane staining was delayed. Cells stained with FM 4-64 and subjected to heat shock displayed a similar effect, with endocytic intermediates becoming more prominent with the severity of the heat shock. For both ethanol stress and heat shock, vacuolar morphology altered from segregated structures to a single, large organelle. The findings of this study reinforce previous observations that ethanol stress and heat shock induce similar responses in yeast.

Authors+Show Affiliations

ICBD, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, U.K. P.G.Meaden@hw.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10487923

Citation

Meaden, P G., et al. "Endocytosis and Vacuolar Morphology in Saccharomyces Cerevisiae Are Altered in Response to Ethanol Stress or Heat Shock." Yeast (Chichester, England), vol. 15, no. 12, 1999, pp. 1211-22.
Meaden PG, Arneborg N, Guldfeldt LU, et al. Endocytosis and vacuolar morphology in Saccharomyces cerevisiae are altered in response to ethanol stress or heat shock. Yeast. 1999;15(12):1211-22.
Meaden, P. G., Arneborg, N., Guldfeldt, L. U., Siegumfeldt, H., & Jakobsen, M. (1999). Endocytosis and vacuolar morphology in Saccharomyces cerevisiae are altered in response to ethanol stress or heat shock. Yeast (Chichester, England), 15(12), 1211-22.
Meaden PG, et al. Endocytosis and Vacuolar Morphology in Saccharomyces Cerevisiae Are Altered in Response to Ethanol Stress or Heat Shock. Yeast. 1999 Sep 15;15(12):1211-22. PubMed PMID: 10487923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endocytosis and vacuolar morphology in Saccharomyces cerevisiae are altered in response to ethanol stress or heat shock. AU - Meaden,P G, AU - Arneborg,N, AU - Guldfeldt,L U, AU - Siegumfeldt,H, AU - Jakobsen,M, PY - 1999/9/17/pubmed PY - 1999/9/17/medline PY - 1999/9/17/entrez SP - 1211 EP - 22 JF - Yeast (Chichester, England) JO - Yeast VL - 15 IS - 12 N2 - The vital lipophilic dye N-(3-triethylammoniumpropyl)-4-[6-(4-(diethylamino)phenyl]hexatrie nyl) pyridinium dibromide (FM 4-64) was used to study the effect of ethanol stress and heat shock on endocytosis in the yeast Saccharomyces cerevisiae. Yeast cells stained with FM 4-64 were placed in a culture chamber and the internalization of the dye was monitored by fluorescence microscopy during perfusion of the cells with fresh growth medium. In the absence of ethanol in the perfusion medium, the internalization of FM 4-64 from the plasma membrane to the vacuolar membrane by yeast cells harvested from the exponential phase of growth was completed in 30 min. The presence of 6% (v/v) ethanol in the perfusion medium had no obvious effect on the internalization of FM 4-64 from the plasma membrane, but did lead to an accumulation of the dye in endocytic intermediates. Consequently, vacuolar membrane staining was delayed. Cells stained with FM 4-64 and subjected to heat shock displayed a similar effect, with endocytic intermediates becoming more prominent with the severity of the heat shock. For both ethanol stress and heat shock, vacuolar morphology altered from segregated structures to a single, large organelle. The findings of this study reinforce previous observations that ethanol stress and heat shock induce similar responses in yeast. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/10487923/Endocytosis_and_vacuolar_morphology_in_Saccharomyces_cerevisiae_are_altered_in_response_to_ethanol_stress_or_heat_shock_ L2 - https://doi.org/10.1002/(SICI)1097-0061(19990915)15:12<1211::AID-YEA448>3.0.CO;2-H DB - PRIME DP - Unbound Medicine ER -