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Differential distribution of four hyperpolarization-activated cation channels in mouse brain.
Biol Chem. 1999 Jul-Aug; 380(7-8):975-80.BC

Abstract

Hyperpolarization-activated cation currents, termed I(h), are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned. The regional expression and cellular localization of the four HCN channel types in mouse brain was investigated using in situ hybridization. The expression of HCN1 was restricted to the olfactory bulb, cerebral cortex, hippocampus, superior colliculus and cerebellum. In contrast, HCN2 transcripts were found at high levels nearly ubiquitously in the brain, and the strongest signals were seen in the olfactory bulb, hippocampus, thalamus and brain stem. HCN3 was uniformly expressed at very low levels throughout the brain. Finally, HCN4 transcripts were prominently expressed selectively in the thalamus and olfactory bulb. Some neurons expressed two or more HCN channel transcripts including hippocampal pyramidal neurons (HCN1, HCN2 and low levels of HCN 4) and thalamic relay neurons (HCN2 and HCN4). Our results demonstrate that each HCN channel transcript has a unique distribution in the brain. Furthermore, they suggest that the heterogeneity of neuronal I(h) may be, at least in part, due to the differential expression of HCN channel genes.

Authors+Show Affiliations

Institut für Pharmakologie und Toxikologie der Technischen Universität München, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10494850

Citation

Moosmang, S, et al. "Differential Distribution of Four Hyperpolarization-activated Cation Channels in Mouse Brain." Biological Chemistry, vol. 380, no. 7-8, 1999, pp. 975-80.
Moosmang S, Biel M, Hofmann F, et al. Differential distribution of four hyperpolarization-activated cation channels in mouse brain. Biol Chem. 1999;380(7-8):975-80.
Moosmang, S., Biel, M., Hofmann, F., & Ludwig, A. (1999). Differential distribution of four hyperpolarization-activated cation channels in mouse brain. Biological Chemistry, 380(7-8), 975-80.
Moosmang S, et al. Differential Distribution of Four Hyperpolarization-activated Cation Channels in Mouse Brain. Biol Chem. 1999 Jul-Aug;380(7-8):975-80. PubMed PMID: 10494850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential distribution of four hyperpolarization-activated cation channels in mouse brain. AU - Moosmang,S, AU - Biel,M, AU - Hofmann,F, AU - Ludwig,A, PY - 1999/9/24/pubmed PY - 1999/9/24/medline PY - 1999/9/24/entrez SP - 975 EP - 80 JF - Biological chemistry JO - Biol Chem VL - 380 IS - 7-8 N2 - Hyperpolarization-activated cation currents, termed I(h), are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned. The regional expression and cellular localization of the four HCN channel types in mouse brain was investigated using in situ hybridization. The expression of HCN1 was restricted to the olfactory bulb, cerebral cortex, hippocampus, superior colliculus and cerebellum. In contrast, HCN2 transcripts were found at high levels nearly ubiquitously in the brain, and the strongest signals were seen in the olfactory bulb, hippocampus, thalamus and brain stem. HCN3 was uniformly expressed at very low levels throughout the brain. Finally, HCN4 transcripts were prominently expressed selectively in the thalamus and olfactory bulb. Some neurons expressed two or more HCN channel transcripts including hippocampal pyramidal neurons (HCN1, HCN2 and low levels of HCN 4) and thalamic relay neurons (HCN2 and HCN4). Our results demonstrate that each HCN channel transcript has a unique distribution in the brain. Furthermore, they suggest that the heterogeneity of neuronal I(h) may be, at least in part, due to the differential expression of HCN channel genes. SN - 1431-6730 UR - https://www.unboundmedicine.com/medline/citation/10494850/Differential_distribution_of_four_hyperpolarization_activated_cation_channels_in_mouse_brain_ L2 - https://www.degruyter.com/document/doi/10.1515/BC.1999.121 DB - PRIME DP - Unbound Medicine ER -