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Prostaglandin E(2) stimulates glutamate receptor-dependent astrocyte neuromodulation in cultured hippocampal cells.
J Neurobiol 1999; 41(2):221-9JN

Abstract

Recent Ca(2+) imaging studies in cell culture and in situ have shown that Ca(2+) elevations in astrocytes stimulate glutamate release and increase neuronal Ca(2+) levels, and that this astrocyte-neuron signaling can be stimulated by prostaglandin E(2) (PGE(2)). We investigated the electrophysiological consequences of the PGE(2)-mediated astrocyte-neuron signaling using whole-cell recordings on cultured rat hippocampal cells. Focal application of PGE(2) to astrocytes evoked a Ca(2+) elevation in the stimulated cell by mobilizing internal Ca(2+) stores, which further propagated as a Ca(2+) wave to neighboring astrocytes. Whole-cell recordings from neurons revealed that PGE(2) evoked a slow inward current in neurons adjacent to astrocytes. This neuronal response required the presence of an astrocyte Ca(2+) wave and was mediated through both N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptors. Taken together with previous studies, these data demonstrate that PGE(2)-evoked Ca(2+) elevations in astrocyte cause the release of glutamate which activates neuronal ionotropic receptors.

Authors+Show Affiliations

Laboratory of Cellular Signaling, Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10512979

Citation

Sanzgiri, R P., et al. "Prostaglandin E(2) Stimulates Glutamate Receptor-dependent Astrocyte Neuromodulation in Cultured Hippocampal Cells." Journal of Neurobiology, vol. 41, no. 2, 1999, pp. 221-9.
Sanzgiri RP, Araque A, Haydon PG. Prostaglandin E(2) stimulates glutamate receptor-dependent astrocyte neuromodulation in cultured hippocampal cells. J Neurobiol. 1999;41(2):221-9.
Sanzgiri, R. P., Araque, A., & Haydon, P. G. (1999). Prostaglandin E(2) stimulates glutamate receptor-dependent astrocyte neuromodulation in cultured hippocampal cells. Journal of Neurobiology, 41(2), pp. 221-9.
Sanzgiri RP, Araque A, Haydon PG. Prostaglandin E(2) Stimulates Glutamate Receptor-dependent Astrocyte Neuromodulation in Cultured Hippocampal Cells. J Neurobiol. 1999 Nov 5;41(2):221-9. PubMed PMID: 10512979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prostaglandin E(2) stimulates glutamate receptor-dependent astrocyte neuromodulation in cultured hippocampal cells. AU - Sanzgiri,R P, AU - Araque,A, AU - Haydon,P G, PY - 1999/10/8/pubmed PY - 1999/10/8/medline PY - 1999/10/8/entrez SP - 221 EP - 9 JF - Journal of neurobiology JO - J. Neurobiol. VL - 41 IS - 2 N2 - Recent Ca(2+) imaging studies in cell culture and in situ have shown that Ca(2+) elevations in astrocytes stimulate glutamate release and increase neuronal Ca(2+) levels, and that this astrocyte-neuron signaling can be stimulated by prostaglandin E(2) (PGE(2)). We investigated the electrophysiological consequences of the PGE(2)-mediated astrocyte-neuron signaling using whole-cell recordings on cultured rat hippocampal cells. Focal application of PGE(2) to astrocytes evoked a Ca(2+) elevation in the stimulated cell by mobilizing internal Ca(2+) stores, which further propagated as a Ca(2+) wave to neighboring astrocytes. Whole-cell recordings from neurons revealed that PGE(2) evoked a slow inward current in neurons adjacent to astrocytes. This neuronal response required the presence of an astrocyte Ca(2+) wave and was mediated through both N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptors. Taken together with previous studies, these data demonstrate that PGE(2)-evoked Ca(2+) elevations in astrocyte cause the release of glutamate which activates neuronal ionotropic receptors. SN - 0022-3034 UR - https://www.unboundmedicine.com/medline/citation/10512979/Prostaglandin_E_2__stimulates_glutamate_receptor_dependent_astrocyte_neuromodulation_in_cultured_hippocampal_cells_ L2 - https://www.lens.org/lens/search?q=citation_id:10512979 DB - PRIME DP - Unbound Medicine ER -