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STRE- and cAMP-independent transcriptional induction of Saccharomyces cerevisiae GSY2 encoding glycogen synthase during diauxic growth on glucose.
Yeast. 1999 Oct; 15(14):1471-84.Y

Abstract

It has been shown that the so-called stationary phase GSY2 gene encoding glycogen synthase was induced as the cells left the exponential phase of growth, while glucose and all other nutrients were still plentiful in the medium (Parrou et al., 1999). Since this effect was essentially controlled at the transcriptional level, we looked for the cis- and trans-acting elements required for this specific growth-related genetic event. We demonstrated that mutations of the HAP2/3/4 binding site and of the two STress-Responsive cis-Elements (STRE) did not abolish the early induction of GSY2, although the latter mutation led to a 20-fold drop in the transcriptional activity of the promoter, as determined from lacZ gene fusions. Insertion of a DNA fragment (from -390 to -167 bp, relative to the ATG) of the promoter lacking the two STREs, upstream to the TATA box of a CYC1-lacZ fusion gene, allowed this reporter gene to be induced with a kinetic similar to that of GSY2-lacZ. Mutations in BCY1, which results in a hyperactive protein kinase A, did not alleviate the early induction, while causing a five- to 10-fold reduction in the transcriptional activity of GSY2. In addition, the repressive effect of protein kinase A was quantitatively conserved when both STREs were mutated in GSY2 promoter, indicating that the negative control of gene expression by the RAS-cAMP signalling pathway does not act solely through STREs. Taken together, these results are indicative of an active process that couples growth control to dynamic glucose consumption.

Authors+Show Affiliations

Parrou JL
Centre de Bioingénierie Gilbert Durand, UMR-CNRS 5504, INRA-UR792, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France.
Enjalbert B
No affiliation info available
François J
No affiliation info available

MeSH

Cyclic AMPCyclic AMP-Dependent Protein KinasesGenes, FungalGlucoseGlycogen SynthaseLac OperonPromoter Regions, GeneticResponse ElementsSaccharomyces cerevisiaeTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10514565

Citation

Parrou, J L., et al. "STRE- and cAMP-independent Transcriptional Induction of Saccharomyces Cerevisiae GSY2 Encoding Glycogen Synthase During Diauxic Growth On Glucose." Yeast (Chichester, England), vol. 15, no. 14, 1999, pp. 1471-84.
Parrou JL, Enjalbert B, François J. STRE- and cAMP-independent transcriptional induction of Saccharomyces cerevisiae GSY2 encoding glycogen synthase during diauxic growth on glucose. Yeast. 1999;15(14):1471-84.
Parrou, J. L., Enjalbert, B., & François, J. (1999). STRE- and cAMP-independent transcriptional induction of Saccharomyces cerevisiae GSY2 encoding glycogen synthase during diauxic growth on glucose. Yeast (Chichester, England), 15(14), 1471-84.
Parrou JL, Enjalbert B, François J. STRE- and cAMP-independent Transcriptional Induction of Saccharomyces Cerevisiae GSY2 Encoding Glycogen Synthase During Diauxic Growth On Glucose. Yeast. 1999;15(14):1471-84. PubMed PMID: 10514565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - STRE- and cAMP-independent transcriptional induction of Saccharomyces cerevisiae GSY2 encoding glycogen synthase during diauxic growth on glucose. AU - Parrou,J L, AU - Enjalbert,B, AU - François,J, PY - 1999/10/9/pubmed PY - 1999/10/9/medline PY - 1999/10/9/entrez SP - 1471 EP - 84 JF - Yeast (Chichester, England) JO - Yeast VL - 15 IS - 14 N2 - It has been shown that the so-called stationary phase GSY2 gene encoding glycogen synthase was induced as the cells left the exponential phase of growth, while glucose and all other nutrients were still plentiful in the medium (Parrou et al., 1999). Since this effect was essentially controlled at the transcriptional level, we looked for the cis- and trans-acting elements required for this specific growth-related genetic event. We demonstrated that mutations of the HAP2/3/4 binding site and of the two STress-Responsive cis-Elements (STRE) did not abolish the early induction of GSY2, although the latter mutation led to a 20-fold drop in the transcriptional activity of the promoter, as determined from lacZ gene fusions. Insertion of a DNA fragment (from -390 to -167 bp, relative to the ATG) of the promoter lacking the two STREs, upstream to the TATA box of a CYC1-lacZ fusion gene, allowed this reporter gene to be induced with a kinetic similar to that of GSY2-lacZ. Mutations in BCY1, which results in a hyperactive protein kinase A, did not alleviate the early induction, while causing a five- to 10-fold reduction in the transcriptional activity of GSY2. In addition, the repressive effect of protein kinase A was quantitatively conserved when both STREs were mutated in GSY2 promoter, indicating that the negative control of gene expression by the RAS-cAMP signalling pathway does not act solely through STREs. Taken together, these results are indicative of an active process that couples growth control to dynamic glucose consumption. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/10514565/STRE__and_cAMP_independent_transcriptional_induction_of_Saccharomyces_cerevisiae_GSY2_encoding_glycogen_synthase_during_diauxic_growth_on_glucose_ L2 - https://doi.org/10.1002/(SICI)1097-0061(199910)15:14<1471::AID-YEA474>3.0.CO;2-Q DB - PRIME DP - Unbound Medicine ER -