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Antiherpetic activity and mode of action of natural carrageenans of diverse structural types.
Antiviral Res. 1999 Sep; 43(2):93-102.AR

Abstract

The lambda-carrageenan 1T1, the kappa/iota-carrageenan 1C1 and the mu/nu-type 1C3, isolated from the red seaweed Gigartina skottsbergii, proved to be potent and selective inhibitors of herpes simplex virus (HSV) types 1 and 2. The antiviral IC50 values determined by virus yield inhibition assay in different cell lines ranged from 0.4 to 3.3 microg/ml, and no cytotoxic effects, measured by trypan blue exclusion on stationary or proliferating cells, tetrazolium salt method or cell protein synthesis, were observed. Time of addition and attachment studies suggested that the main target for antiviral action of the three carrageenans was virus adsorption, whereas no effect on virus internalization, or early or late protein synthesis was detected. However, the lambda-carrageenan 1T1 was still significantly inhibitory when added any time after adsorption. The pretreatment of virions with the carrageenans showed that 1C1 and 1C3 lacked direct inactivating effect at concentrations near the antiviral IC50 but 1T1 exerted virucidal action. The cyclization of 1T1 to afford the derivative 1T1T1 maintained the antiviral activity but eliminated the virucidal properties. Thus, the structure of 1T1 seems to be responsible for its differential behavior from 1C1 and 1C3, probably allowing a more stable binding to HSV, leading to virion inactivation. In contrast, 1C1 and 1C3 fail to bind with high affinity to virus alone, but are able to interfere with the interaction between HSV particles and the cell.

Authors+Show Affiliations

Carlucci MJ
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.
Ciancia M
No affiliation info available
Matulewicz MC
No affiliation info available
Cerezo AS
No affiliation info available
Damonte EB
No affiliation info available

MeSH

AdsorptionAnimalsAntiviral AgentsCarrageenanCell LineCell SurvivalChlorocebus aethiopsHerpesvirus 1, HumanHerpesvirus 2, HumanHumansInhibitory Concentration 50SeaweedSimplexvirusVero CellsVirionVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10517311

Citation

Carlucci, M J., et al. "Antiherpetic Activity and Mode of Action of Natural Carrageenans of Diverse Structural Types." Antiviral Research, vol. 43, no. 2, 1999, pp. 93-102.
Carlucci MJ, Ciancia M, Matulewicz MC, et al. Antiherpetic activity and mode of action of natural carrageenans of diverse structural types. Antiviral Res. 1999;43(2):93-102.
Carlucci, M. J., Ciancia, M., Matulewicz, M. C., Cerezo, A. S., & Damonte, E. B. (1999). Antiherpetic activity and mode of action of natural carrageenans of diverse structural types. Antiviral Research, 43(2), 93-102.
Carlucci MJ, et al. Antiherpetic Activity and Mode of Action of Natural Carrageenans of Diverse Structural Types. Antiviral Res. 1999;43(2):93-102. PubMed PMID: 10517311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiherpetic activity and mode of action of natural carrageenans of diverse structural types. AU - Carlucci,M J, AU - Ciancia,M, AU - Matulewicz,M C, AU - Cerezo,A S, AU - Damonte,E B, PY - 1999/10/12/pubmed PY - 1999/10/12/medline PY - 1999/10/12/entrez SP - 93 EP - 102 JF - Antiviral research JO - Antiviral Res VL - 43 IS - 2 N2 - The lambda-carrageenan 1T1, the kappa/iota-carrageenan 1C1 and the mu/nu-type 1C3, isolated from the red seaweed Gigartina skottsbergii, proved to be potent and selective inhibitors of herpes simplex virus (HSV) types 1 and 2. The antiviral IC50 values determined by virus yield inhibition assay in different cell lines ranged from 0.4 to 3.3 microg/ml, and no cytotoxic effects, measured by trypan blue exclusion on stationary or proliferating cells, tetrazolium salt method or cell protein synthesis, were observed. Time of addition and attachment studies suggested that the main target for antiviral action of the three carrageenans was virus adsorption, whereas no effect on virus internalization, or early or late protein synthesis was detected. However, the lambda-carrageenan 1T1 was still significantly inhibitory when added any time after adsorption. The pretreatment of virions with the carrageenans showed that 1C1 and 1C3 lacked direct inactivating effect at concentrations near the antiviral IC50 but 1T1 exerted virucidal action. The cyclization of 1T1 to afford the derivative 1T1T1 maintained the antiviral activity but eliminated the virucidal properties. Thus, the structure of 1T1 seems to be responsible for its differential behavior from 1C1 and 1C3, probably allowing a more stable binding to HSV, leading to virion inactivation. In contrast, 1C1 and 1C3 fail to bind with high affinity to virus alone, but are able to interfere with the interaction between HSV particles and the cell. SN - 0166-3542 UR - https://www.unboundmedicine.com/medline/citation/10517311/Antiherpetic_activity_and_mode_of_action_of_natural_carrageenans_of_diverse_structural_types_ DB - PRIME DP - Unbound Medicine ER -