Tags

Type your tag names separated by a space and hit enter

Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant.
J Biol Chem 1999; 274(43):31020-4JB

Abstract

Both the secAcsR11 and DeltasecG::kan mutations cause cold-sensitive growth, although the growth defect due to the latter mutation occurs in a strain-specific manner. Overexpression of pgsA encoding phosphatidylglycerophosphate synthase suppresses the growth defects of the two mutants. We investigated the mechanism underlying the pgsA-dependent suppression of the two mutations using purified mutant SecA and inverted membrane vesicles (IMVs) prepared from pgsA-overexpressing cells. The acidic phospholipid content increased by about 10% upon pgsA overexpression. This increase resulted in the stimulation of proOmpA translocation only when mutant SecA or SecG-depleted IMVs were used. The translocation-coupled ATPase activity of SecA was significantly defective with the mutant SecA or SecG-depleted IMVs, but it recovered to a near normal level when the acidic phospholipid level was increased. The stimulation of ATPase activity was observed only at low temperature. The steady-state level of membrane-inserted SecA was low with the mutant SecA or SecG-depleted IMVs, and it decreased further upon the increase in the acidic phospholipid content. However, the level of SecA insertion markedly increased upon the inhibition of SecA deinsertion by the addition of beta,gamma-imido adenosine 5'-triphosphate (AMP-PNP), especially with IMVs containing increased levels of acidic phospholipids. These results indicate that the increase in the level of acidic phospholipids stimulates the SecA cycle in the two mutants by facilitating both the insertion and deinsertion of SecA.

Authors+Show Affiliations

Institute of Molecular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10521500

Citation

Suzuki, H, et al. "Increases in Acidic Phospholipid Contents Specifically Restore Protein Translocation in a Cold-sensitive secA or secG Null Mutant." The Journal of Biological Chemistry, vol. 274, no. 43, 1999, pp. 31020-4.
Suzuki H, Nishiyama K, Tokuda H. Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant. J Biol Chem. 1999;274(43):31020-4.
Suzuki, H., Nishiyama, K., & Tokuda, H. (1999). Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant. The Journal of Biological Chemistry, 274(43), pp. 31020-4.
Suzuki H, Nishiyama K, Tokuda H. Increases in Acidic Phospholipid Contents Specifically Restore Protein Translocation in a Cold-sensitive secA or secG Null Mutant. J Biol Chem. 1999 Oct 22;274(43):31020-4. PubMed PMID: 10521500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increases in acidic phospholipid contents specifically restore protein translocation in a cold-sensitive secA or secG null mutant. AU - Suzuki,H, AU - Nishiyama,K, AU - Tokuda,H, PY - 1999/10/16/pubmed PY - 1999/10/16/medline PY - 1999/10/16/entrez SP - 31020 EP - 4 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 274 IS - 43 N2 - Both the secAcsR11 and DeltasecG::kan mutations cause cold-sensitive growth, although the growth defect due to the latter mutation occurs in a strain-specific manner. Overexpression of pgsA encoding phosphatidylglycerophosphate synthase suppresses the growth defects of the two mutants. We investigated the mechanism underlying the pgsA-dependent suppression of the two mutations using purified mutant SecA and inverted membrane vesicles (IMVs) prepared from pgsA-overexpressing cells. The acidic phospholipid content increased by about 10% upon pgsA overexpression. This increase resulted in the stimulation of proOmpA translocation only when mutant SecA or SecG-depleted IMVs were used. The translocation-coupled ATPase activity of SecA was significantly defective with the mutant SecA or SecG-depleted IMVs, but it recovered to a near normal level when the acidic phospholipid level was increased. The stimulation of ATPase activity was observed only at low temperature. The steady-state level of membrane-inserted SecA was low with the mutant SecA or SecG-depleted IMVs, and it decreased further upon the increase in the acidic phospholipid content. However, the level of SecA insertion markedly increased upon the inhibition of SecA deinsertion by the addition of beta,gamma-imido adenosine 5'-triphosphate (AMP-PNP), especially with IMVs containing increased levels of acidic phospholipids. These results indicate that the increase in the level of acidic phospholipids stimulates the SecA cycle in the two mutants by facilitating both the insertion and deinsertion of SecA. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/10521500/Increases_in_acidic_phospholipid_contents_specifically_restore_protein_translocation_in_a_cold_sensitive_secA_or_secG_null_mutant_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=10521500 DB - PRIME DP - Unbound Medicine ER -