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Detection of hepatitis B virus DNA in tissues of hepatocellular carcinomas related to hepatitis C virus which are negative for hepatitis B virus surface antigen.
Scand J Gastroenterol. 1999 Sep; 34(9):934-8.SJ

Abstract

BACKGROUND

Uniform conclusions have not yet been drawn as to the frequency and copy number of hepatitis B virus (HBV) DNA in hepatocellular carcinoma (HCC) tissues from patients who are negative for hepatitis B surface antigen (HBsAg).

METHODS

The existence of HBV DNA was investigated with Southern blot hybridization and polymerase chain reaction (PCR) in HCC tissues from 55 patients who were negative for HBsAg and positive for hepatitis C virus antibody (HCVAb).

RESULTS

Southern blot hybridization showed that two tissues (4%) contained HBV DNA at a frequency of more than 1 HBV copy per 10 cells. Another 5 tissues were found to be positive for HBV DNA only by PCR analysis but contained less than 3 HBV DNA copies per 10(5) cells.

CONCLUSIONS

HCC tissues from patients seronegative for HBsAg and seropositive for HCVAb contain HBV DNA less frequently than has generally been claimed.

Authors+Show Affiliations

Dept of Virology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10522615

Citation

Sugawara, Y, et al. "Detection of Hepatitis B Virus DNA in Tissues of Hepatocellular Carcinomas Related to Hepatitis C Virus Which Are Negative for Hepatitis B Virus Surface Antigen." Scandinavian Journal of Gastroenterology, vol. 34, no. 9, 1999, pp. 934-8.
Sugawara Y, Makuuchi M, Takada K. Detection of hepatitis B virus DNA in tissues of hepatocellular carcinomas related to hepatitis C virus which are negative for hepatitis B virus surface antigen. Scand J Gastroenterol. 1999;34(9):934-8.
Sugawara, Y., Makuuchi, M., & Takada, K. (1999). Detection of hepatitis B virus DNA in tissues of hepatocellular carcinomas related to hepatitis C virus which are negative for hepatitis B virus surface antigen. Scandinavian Journal of Gastroenterology, 34(9), 934-8.
Sugawara Y, Makuuchi M, Takada K. Detection of Hepatitis B Virus DNA in Tissues of Hepatocellular Carcinomas Related to Hepatitis C Virus Which Are Negative for Hepatitis B Virus Surface Antigen. Scand J Gastroenterol. 1999;34(9):934-8. PubMed PMID: 10522615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of hepatitis B virus DNA in tissues of hepatocellular carcinomas related to hepatitis C virus which are negative for hepatitis B virus surface antigen. AU - Sugawara,Y, AU - Makuuchi,M, AU - Takada,K, PY - 1999/10/16/pubmed PY - 1999/10/16/medline PY - 1999/10/16/entrez SP - 934 EP - 8 JF - Scandinavian journal of gastroenterology JO - Scand. J. Gastroenterol. VL - 34 IS - 9 N2 - BACKGROUND: Uniform conclusions have not yet been drawn as to the frequency and copy number of hepatitis B virus (HBV) DNA in hepatocellular carcinoma (HCC) tissues from patients who are negative for hepatitis B surface antigen (HBsAg). METHODS: The existence of HBV DNA was investigated with Southern blot hybridization and polymerase chain reaction (PCR) in HCC tissues from 55 patients who were negative for HBsAg and positive for hepatitis C virus antibody (HCVAb). RESULTS: Southern blot hybridization showed that two tissues (4%) contained HBV DNA at a frequency of more than 1 HBV copy per 10 cells. Another 5 tissues were found to be positive for HBV DNA only by PCR analysis but contained less than 3 HBV DNA copies per 10(5) cells. CONCLUSIONS: HCC tissues from patients seronegative for HBsAg and seropositive for HCVAb contain HBV DNA less frequently than has generally been claimed. SN - 0036-5521 UR - https://www.unboundmedicine.com/medline/citation/10522615/Detection_of_hepatitis_B_virus_DNA_in_tissues_of_hepatocellular_carcinomas_related_to_hepatitis_C_virus_which_are_negative_for_hepatitis_B_virus_surface_antigen_ L2 - http://www.tandfonline.com/doi/full/10.1080/003655299750025435 DB - PRIME DP - Unbound Medicine ER -