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Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome.
J Clin Microbiol. 1999 Nov; 37(11):3491-6.JC

Abstract

We have isolated one sorbitol-nonfermenting (SNF) Escherichia coli O157:H7 isolate and five sorbitol-fermenting (SF) E. coli O157:H(-) isolates that do not contain Shiga toxin (Stx) genes (stx). Isolates originated from patients with diarrhea (n = 4) and hemolytic-uremic syndrome (HUS) (n = 2). All isolates harbored a chromosomal eae gene encoding gamma-intimin as well as the plasmid genes E-hly and etp. The E. coli O157:H7 isolate was katP and espP positive. Respective sera obtained from the patient with HUS contained antibodies to the O157 lipopolysaccharide antigen. The stx-negative E. coli O157:H7 isolate is genetically related to stx-positive SNF E. coli O157:H7. All stx-negative SF E. coli O157:H(-) isolates belong to the same genetic cluster and are closely related to stx-positive SF E. coli O157:H(-) isolates. Our data indicate that stx-negative E. coli O157:H7/H(-) variants may occur at a low frequency and cannot be recognized by diagnostic methods that target Stx.

Authors+Show Affiliations

Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10523540

Citation

Schmidt, H, et al. "Escherichia Coli O157:H7 and O157:H(-) Strains That Do Not Produce Shiga Toxin: Phenotypic and Genetic Characterization of Isolates Associated With Diarrhea and Hemolytic-uremic Syndrome." Journal of Clinical Microbiology, vol. 37, no. 11, 1999, pp. 3491-6.
Schmidt H, Scheef J, Huppertz HI, et al. Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome. J Clin Microbiol. 1999;37(11):3491-6.
Schmidt, H., Scheef, J., Huppertz, H. I., Frosch, M., & Karch, H. (1999). Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome. Journal of Clinical Microbiology, 37(11), 3491-6.
Schmidt H, et al. Escherichia Coli O157:H7 and O157:H(-) Strains That Do Not Produce Shiga Toxin: Phenotypic and Genetic Characterization of Isolates Associated With Diarrhea and Hemolytic-uremic Syndrome. J Clin Microbiol. 1999;37(11):3491-6. PubMed PMID: 10523540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome. AU - Schmidt,H, AU - Scheef,J, AU - Huppertz,H I, AU - Frosch,M, AU - Karch,H, PY - 1999/10/19/pubmed PY - 1999/10/19/medline PY - 1999/10/19/entrez SP - 3491 EP - 6 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 37 IS - 11 N2 - We have isolated one sorbitol-nonfermenting (SNF) Escherichia coli O157:H7 isolate and five sorbitol-fermenting (SF) E. coli O157:H(-) isolates that do not contain Shiga toxin (Stx) genes (stx). Isolates originated from patients with diarrhea (n = 4) and hemolytic-uremic syndrome (HUS) (n = 2). All isolates harbored a chromosomal eae gene encoding gamma-intimin as well as the plasmid genes E-hly and etp. The E. coli O157:H7 isolate was katP and espP positive. Respective sera obtained from the patient with HUS contained antibodies to the O157 lipopolysaccharide antigen. The stx-negative E. coli O157:H7 isolate is genetically related to stx-positive SNF E. coli O157:H7. All stx-negative SF E. coli O157:H(-) isolates belong to the same genetic cluster and are closely related to stx-positive SF E. coli O157:H(-) isolates. Our data indicate that stx-negative E. coli O157:H7/H(-) variants may occur at a low frequency and cannot be recognized by diagnostic methods that target Stx. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/10523540/Escherichia_coli_O157:H7_and_O157:H____strains_that_do_not_produce_Shiga_toxin:_phenotypic_and_genetic_characterization_of_isolates_associated_with_diarrhea_and_hemolytic_uremic_syndrome_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=10523540 DB - PRIME DP - Unbound Medicine ER -