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Human hematopoietic stem/progenitor cells generate CD5+ B lymphoid cells in NOD/SCID mice.
Stem Cells. 1999; 17(5):242-52.SC

Abstract

The nonobese diabetic/severe combined immunodeficient (NOD/SCID) xenotransplantation model is increasingly utilized to study both human lymphohematopoietic stem/progenitor cells and committed cell types. Human B lymphoid cells develop and proliferate in this model. We found high numbers of CD19+CD5+ B lymphoid cells in the bone marrows and spleens of NOD/SCID mice transplanted with human CD34+ stem/progenitor cells. The CD5+ cells accounted for a particularly large percentage of the B lymphoid cells in the spleens of chimeras analyzed three months after transplantation. CD19+CD5+ cells from all the analyzed chimeras coexpressed HLA-DR, surface IgM, CD20, CD38, CD43, and CD45. However, CD19+CD5+ cells were negative for kappa light chain, CD10, CD11a, CD11b, CD15, CD21, CD22, CD23, CD25, CD34, CD35, CD44, CD62L, CD69, and CD71. Cell surface expression of the lambda light chain, surface IgD, CD9, and CD40 antigens was detected in some but not all chimeras. Thus, the CD19+CD5+ cell population detected in our study has the phenotype of previously described CD5+ B lymphoid cells in humans and other species. The origin and role of the B lymphoid cells which express CD5 cell surface glycoprotein are poorly understood. The malignant cells in B lymphoid chronic lymphocytic leukemia express CD5, and the numbers of CD5+ B lymphoid cells are elevated in several autoimmune conditions. The human-NOD/SCID chimera system may provide an in vivo model to investigate the maturation and development of this cryptic human CD5+ B lymphoid cell subpopulation.

Authors+Show Affiliations

Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10527459

Citation

Novelli, E M., et al. "Human Hematopoietic Stem/progenitor Cells Generate CD5+ B Lymphoid Cells in NOD/SCID Mice." Stem Cells (Dayton, Ohio), vol. 17, no. 5, 1999, pp. 242-52.
Novelli EM, Ramírez M, Leung W, et al. Human hematopoietic stem/progenitor cells generate CD5+ B lymphoid cells in NOD/SCID mice. Stem Cells. 1999;17(5):242-52.
Novelli, E. M., Ramírez, M., Leung, W., & Civin, C. I. (1999). Human hematopoietic stem/progenitor cells generate CD5+ B lymphoid cells in NOD/SCID mice. Stem Cells (Dayton, Ohio), 17(5), 242-52.
Novelli EM, et al. Human Hematopoietic Stem/progenitor Cells Generate CD5+ B Lymphoid Cells in NOD/SCID Mice. Stem Cells. 1999;17(5):242-52. PubMed PMID: 10527459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human hematopoietic stem/progenitor cells generate CD5+ B lymphoid cells in NOD/SCID mice. AU - Novelli,E M, AU - Ramírez,M, AU - Leung,W, AU - Civin,C I, PY - 1999/10/20/pubmed PY - 1999/10/20/medline PY - 1999/10/20/entrez SP - 242 EP - 52 JF - Stem cells (Dayton, Ohio) JO - Stem Cells VL - 17 IS - 5 N2 - The nonobese diabetic/severe combined immunodeficient (NOD/SCID) xenotransplantation model is increasingly utilized to study both human lymphohematopoietic stem/progenitor cells and committed cell types. Human B lymphoid cells develop and proliferate in this model. We found high numbers of CD19+CD5+ B lymphoid cells in the bone marrows and spleens of NOD/SCID mice transplanted with human CD34+ stem/progenitor cells. The CD5+ cells accounted for a particularly large percentage of the B lymphoid cells in the spleens of chimeras analyzed three months after transplantation. CD19+CD5+ cells from all the analyzed chimeras coexpressed HLA-DR, surface IgM, CD20, CD38, CD43, and CD45. However, CD19+CD5+ cells were negative for kappa light chain, CD10, CD11a, CD11b, CD15, CD21, CD22, CD23, CD25, CD34, CD35, CD44, CD62L, CD69, and CD71. Cell surface expression of the lambda light chain, surface IgD, CD9, and CD40 antigens was detected in some but not all chimeras. Thus, the CD19+CD5+ cell population detected in our study has the phenotype of previously described CD5+ B lymphoid cells in humans and other species. The origin and role of the B lymphoid cells which express CD5 cell surface glycoprotein are poorly understood. The malignant cells in B lymphoid chronic lymphocytic leukemia express CD5, and the numbers of CD5+ B lymphoid cells are elevated in several autoimmune conditions. The human-NOD/SCID chimera system may provide an in vivo model to investigate the maturation and development of this cryptic human CD5+ B lymphoid cell subpopulation. SN - 1066-5099 UR - https://www.unboundmedicine.com/medline/citation/10527459/Human_hematopoietic_stem/progenitor_cells_generate_CD5+_B_lymphoid_cells_in_NOD/SCID_mice_ L2 - https://doi.org/10.1002/stem.170242 DB - PRIME DP - Unbound Medicine ER -