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Differential expression of ileal Na(+)/H(+) exchanger isoforms after enterectomy.
J Surg Res. 1999 Oct; 86(2):192-7.JS

Abstract

BACKGROUND

Na(+)/H(+) exchangers (NHE) are transporters involved in the absorption of NaCl along the gastrointestinal tract. The aim of this study was to determine the expression pattern of the intestinal brush border NHE isoforms 2 and 3 following massive small bowel resection (SBR). Additionally, the effect of epidermal growth factor (EGF) and salivarectomy (removal of the primary source of EGF) on the expression pattern was studied.

MATERIALS AND METHODS

ICR mice underwent a proximal SBR or sham surgery and then received either orogastric saline or EGF (50 microg/kg/day). In separate experiments mice underwent salivarectomy followed by SBR or sham. Postoperatively the remaining ileum was isolated and levels of NHE-2 and NHE-3 mRNA and protein were resolved.

RESULTS

Following SBR, the expression of both mRNA and protein for NHE-3 increased by approximately 2.5-fold. Treatment with EGF enhanced NHE-3 mRNA in sham animals with further elevation following SBR. The expression of NHE-2 mRNA demonstrated minimal change while protein marginally increased (40%) following SBR. EGF did not affect the expression of NHE-2 mRNA. Salivarectomy did not influence NHE-2 protein expression and inhibited the increased NHE-3 protein expression following SBR.

CONCLUSIONS

Following SBR, the expression pattern for brush border NHE isoforms is distinctive. Increased expression of NHE-3 secondary to SBR and/or EGF treatment with loss of this increase following salivarectomy implies a common mechanism to enhance enterocyte proliferation and luminal absorption of NaCl and water. These results suggest that NHE-3 is an important ileal exchanger following SBR.

Authors+Show Affiliations

Department of Surgery, Children's Hospital Medical Center, Cincinnati, Ohio, 45229, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10534423

Citation

Falcone, R A., et al. "Differential Expression of Ileal Na(+)/H(+) Exchanger Isoforms After Enterectomy." The Journal of Surgical Research, vol. 86, no. 2, 1999, pp. 192-7.
Falcone RA, Shin CE, Stern LE, et al. Differential expression of ileal Na(+)/H(+) exchanger isoforms after enterectomy. J Surg Res. 1999;86(2):192-7.
Falcone, R. A., Shin, C. E., Stern, L. E., Wang, Z., Erwin, C. R., Soleimani, M., & Warner, B. W. (1999). Differential expression of ileal Na(+)/H(+) exchanger isoforms after enterectomy. The Journal of Surgical Research, 86(2), 192-7.
Falcone RA, et al. Differential Expression of Ileal Na(+)/H(+) Exchanger Isoforms After Enterectomy. J Surg Res. 1999;86(2):192-7. PubMed PMID: 10534423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of ileal Na(+)/H(+) exchanger isoforms after enterectomy. AU - Falcone,R A,Jr AU - Shin,C E, AU - Stern,L E, AU - Wang,Z, AU - Erwin,C R, AU - Soleimani,M, AU - Warner,B W, PY - 1999/10/27/pubmed PY - 2001/3/28/medline PY - 1999/10/27/entrez SP - 192 EP - 7 JF - The Journal of surgical research JO - J Surg Res VL - 86 IS - 2 N2 - BACKGROUND: Na(+)/H(+) exchangers (NHE) are transporters involved in the absorption of NaCl along the gastrointestinal tract. The aim of this study was to determine the expression pattern of the intestinal brush border NHE isoforms 2 and 3 following massive small bowel resection (SBR). Additionally, the effect of epidermal growth factor (EGF) and salivarectomy (removal of the primary source of EGF) on the expression pattern was studied. MATERIALS AND METHODS: ICR mice underwent a proximal SBR or sham surgery and then received either orogastric saline or EGF (50 microg/kg/day). In separate experiments mice underwent salivarectomy followed by SBR or sham. Postoperatively the remaining ileum was isolated and levels of NHE-2 and NHE-3 mRNA and protein were resolved. RESULTS: Following SBR, the expression of both mRNA and protein for NHE-3 increased by approximately 2.5-fold. Treatment with EGF enhanced NHE-3 mRNA in sham animals with further elevation following SBR. The expression of NHE-2 mRNA demonstrated minimal change while protein marginally increased (40%) following SBR. EGF did not affect the expression of NHE-2 mRNA. Salivarectomy did not influence NHE-2 protein expression and inhibited the increased NHE-3 protein expression following SBR. CONCLUSIONS: Following SBR, the expression pattern for brush border NHE isoforms is distinctive. Increased expression of NHE-3 secondary to SBR and/or EGF treatment with loss of this increase following salivarectomy implies a common mechanism to enhance enterocyte proliferation and luminal absorption of NaCl and water. These results suggest that NHE-3 is an important ileal exchanger following SBR. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/10534423/Differential_expression_of_ileal_Na_+_/H_+__exchanger_isoforms_after_enterectomy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(99)95720-7 DB - PRIME DP - Unbound Medicine ER -