Tags

Type your tag names separated by a space and hit enter

Effect of alpha-phenyl-N-tert-butylnitrone on diabetes and lipid peroxidation in BB rats.
Can J Physiol Pharmacol 1999; 77(3):166-74CJ

Abstract

Oxygen free radicals have been shown to interfere with pancreatic islet beta cell function and integrity, and have been implicated in autoimmune type 1 diabetes. We hypothesized that the spontaneous autoimmune type 1 diabetes of the BB rat would be prevented by in vivo administration of a free-radical spin trap, alpha-phenyl-N-tert-butylnitrone (PBN). Twenty-eight diabetes-prone (BBdp) and 13 non-diabetes-prone (BBn) rats received PBN (10 mg/kg) subcutaneously twice daily, and 27 BBdp and 12 BBn rats received saline as controls. Rats were treated from age 47 +/- 6 days until diabetes onset or age 118 +/- 7 days. PBN caused no growth, biochemical, or hematological side effects. Sixteen control BBdp rats became diabetic (BBd, mean age 77 +/- 6 days) and six demonstrated impaired glucose tolerance (IGT rats). The incidence of diabetes and IGT was not different in PBN-treated BBdp rats. Saline-treated rats showed no differences in pancreatic malondialdehyde (MDA) contents of BBd, IGT rats, and the BBdp that did not develop diabetes, versus BBn rats (2.38 +/- 0.35 nmoL/g). Among rats receiving PBN, BBn had lower pancreatic MDA than BBd and IGT rats (1.38 +/- 0.15 vs. 1.88 +/- 0.15 and 2.02 +/- 0.24 nmoL/g, p < 0.05), but not than BBdp rats (1.78 +/- 0.12 nmoL/g, ns). BBn rats receiving PBN also had lower pancreatic MDA than the saline controls (p < 0.05). Thus, PBN is remarkably nontoxic and is able to decrease MDA in the absence of the autoimmune process, but does not prevent diabetes. A combination of PBN with other complementary antioxidant agents may hold better promise for disease prevention.

Authors+Show Affiliations

McGill Nutrition and Food Science Centre, Royal Victoria Hospital, Montréal, QC, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10535689

Citation

Iovino, G, et al. "Effect of alpha-phenyl-N-tert-butylnitrone On Diabetes and Lipid Peroxidation in BB Rats." Canadian Journal of Physiology and Pharmacology, vol. 77, no. 3, 1999, pp. 166-74.
Iovino G, Kubow S, Marliss EB. Effect of alpha-phenyl-N-tert-butylnitrone on diabetes and lipid peroxidation in BB rats. Can J Physiol Pharmacol. 1999;77(3):166-74.
Iovino, G., Kubow, S., & Marliss, E. B. (1999). Effect of alpha-phenyl-N-tert-butylnitrone on diabetes and lipid peroxidation in BB rats. Canadian Journal of Physiology and Pharmacology, 77(3), pp. 166-74.
Iovino G, Kubow S, Marliss EB. Effect of alpha-phenyl-N-tert-butylnitrone On Diabetes and Lipid Peroxidation in BB Rats. Can J Physiol Pharmacol. 1999;77(3):166-74. PubMed PMID: 10535689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of alpha-phenyl-N-tert-butylnitrone on diabetes and lipid peroxidation in BB rats. AU - Iovino,G, AU - Kubow,S, AU - Marliss,E B, PY - 1999/10/27/pubmed PY - 1999/10/27/medline PY - 1999/10/27/entrez SP - 166 EP - 74 JF - Canadian journal of physiology and pharmacology JO - Can. J. Physiol. Pharmacol. VL - 77 IS - 3 N2 - Oxygen free radicals have been shown to interfere with pancreatic islet beta cell function and integrity, and have been implicated in autoimmune type 1 diabetes. We hypothesized that the spontaneous autoimmune type 1 diabetes of the BB rat would be prevented by in vivo administration of a free-radical spin trap, alpha-phenyl-N-tert-butylnitrone (PBN). Twenty-eight diabetes-prone (BBdp) and 13 non-diabetes-prone (BBn) rats received PBN (10 mg/kg) subcutaneously twice daily, and 27 BBdp and 12 BBn rats received saline as controls. Rats were treated from age 47 +/- 6 days until diabetes onset or age 118 +/- 7 days. PBN caused no growth, biochemical, or hematological side effects. Sixteen control BBdp rats became diabetic (BBd, mean age 77 +/- 6 days) and six demonstrated impaired glucose tolerance (IGT rats). The incidence of diabetes and IGT was not different in PBN-treated BBdp rats. Saline-treated rats showed no differences in pancreatic malondialdehyde (MDA) contents of BBd, IGT rats, and the BBdp that did not develop diabetes, versus BBn rats (2.38 +/- 0.35 nmoL/g). Among rats receiving PBN, BBn had lower pancreatic MDA than BBd and IGT rats (1.38 +/- 0.15 vs. 1.88 +/- 0.15 and 2.02 +/- 0.24 nmoL/g, p < 0.05), but not than BBdp rats (1.78 +/- 0.12 nmoL/g, ns). BBn rats receiving PBN also had lower pancreatic MDA than the saline controls (p < 0.05). Thus, PBN is remarkably nontoxic and is able to decrease MDA in the absence of the autoimmune process, but does not prevent diabetes. A combination of PBN with other complementary antioxidant agents may hold better promise for disease prevention. SN - 0008-4212 UR - https://www.unboundmedicine.com/medline/citation/10535689/Effect_of_alpha_phenyl_N_tert_butylnitrone_on_diabetes_and_lipid_peroxidation_in_BB_rats_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&amp;PAGE=linkout&amp;SEARCH=10535689.ui DB - PRIME DP - Unbound Medicine ER -