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Risk factors for Ki-ras protooncogene mutation in sporadic colorectal adenomas.
Cancer Res. 1999 Oct 15; 59(20):5181-5.CR

Abstract

The Ki-ras protooncogene frequently is mutated in colorectal adenocarcinomas, but the etiology of this molecular event is uncertain. We investigated the association between variables known or suspected to be related to risk for colorectal cancer and the occurrence of Ki-ras mutations in colorectal adenomas. This study was conducted among 678 male and female participants, 40-80 years of age, enrolled in a phase III trial testing the effects of a wheat bran fiber supplement on adenoma recurrence. Exposure information on the risk factors of interest was assessed through self-administered questionnaires. Mutations in codons 12 and 13 of the Ki-ras protooncogene were analyzed in baseline adenomas 0.5 cm or larger by PCR amplification followed by direct sequencing. Eighteen percent (120 of 678) of the participants had one or more adenoma(s) with Ki-ras mutations. A higher risk of Ki-ras mutations was associated with increasing age and a lower intake of total folate. The odds ratio (OR) for Ki-ras mutations for individuals >72 years of age was 1.98 [95% confidence interval (CI) = 1.19-3.27; P for trend = 0.008] compared with those less than 65 years of age. Compared with individuals in the lower tertile of total folate, those in the upper tertile had an approximately 50% lower risk of having Ki-ras mutation-positive adenomas (OR = 0.52; 95% CI = 0.30-0.88; P for trend = 0.02). There was a suggestion of a stronger inverse association of total folate with G-->T transversions (OR = 0.41; 95% CI = 0.20-0.87) than G-->A transitions (OR = 0.61; 95% CI = 0.31-1.21), although the CIs for the associations overlap. The results of these analyses suggest that the protective effect of folate in colon cancer observed in published studies may be mediated through folate's effect on Ki-ras mutations.

Authors+Show Affiliations

Arizona Cancer Center, University of Arizona, Tucson 85724, USA. emartinez@azcc.arizona.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10537295

Citation

Martínez, M E., et al. "Risk Factors for Ki-ras Protooncogene Mutation in Sporadic Colorectal Adenomas." Cancer Research, vol. 59, no. 20, 1999, pp. 5181-5.
Martínez ME, Maltzman T, Marshall JR, et al. Risk factors for Ki-ras protooncogene mutation in sporadic colorectal adenomas. Cancer Res. 1999;59(20):5181-5.
Martínez, M. E., Maltzman, T., Marshall, J. R., Einspahr, J., Reid, M. E., Sampliner, R., Ahnen, D. J., Hamilton, S. R., & Alberts, D. S. (1999). Risk factors for Ki-ras protooncogene mutation in sporadic colorectal adenomas. Cancer Research, 59(20), 5181-5.
Martínez ME, et al. Risk Factors for Ki-ras Protooncogene Mutation in Sporadic Colorectal Adenomas. Cancer Res. 1999 Oct 15;59(20):5181-5. PubMed PMID: 10537295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Risk factors for Ki-ras protooncogene mutation in sporadic colorectal adenomas. AU - Martínez,M E, AU - Maltzman,T, AU - Marshall,J R, AU - Einspahr,J, AU - Reid,M E, AU - Sampliner,R, AU - Ahnen,D J, AU - Hamilton,S R, AU - Alberts,D S, PY - 1999/10/28/pubmed PY - 1999/10/28/medline PY - 1999/10/28/entrez SP - 5181 EP - 5 JF - Cancer research JO - Cancer Res. VL - 59 IS - 20 N2 - The Ki-ras protooncogene frequently is mutated in colorectal adenocarcinomas, but the etiology of this molecular event is uncertain. We investigated the association between variables known or suspected to be related to risk for colorectal cancer and the occurrence of Ki-ras mutations in colorectal adenomas. This study was conducted among 678 male and female participants, 40-80 years of age, enrolled in a phase III trial testing the effects of a wheat bran fiber supplement on adenoma recurrence. Exposure information on the risk factors of interest was assessed through self-administered questionnaires. Mutations in codons 12 and 13 of the Ki-ras protooncogene were analyzed in baseline adenomas 0.5 cm or larger by PCR amplification followed by direct sequencing. Eighteen percent (120 of 678) of the participants had one or more adenoma(s) with Ki-ras mutations. A higher risk of Ki-ras mutations was associated with increasing age and a lower intake of total folate. The odds ratio (OR) for Ki-ras mutations for individuals >72 years of age was 1.98 [95% confidence interval (CI) = 1.19-3.27; P for trend = 0.008] compared with those less than 65 years of age. Compared with individuals in the lower tertile of total folate, those in the upper tertile had an approximately 50% lower risk of having Ki-ras mutation-positive adenomas (OR = 0.52; 95% CI = 0.30-0.88; P for trend = 0.02). There was a suggestion of a stronger inverse association of total folate with G-->T transversions (OR = 0.41; 95% CI = 0.20-0.87) than G-->A transitions (OR = 0.61; 95% CI = 0.31-1.21), although the CIs for the associations overlap. The results of these analyses suggest that the protective effect of folate in colon cancer observed in published studies may be mediated through folate's effect on Ki-ras mutations. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/10537295/Risk_factors_for_Ki_ras_protooncogene_mutation_in_sporadic_colorectal_adenomas_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=10537295 DB - PRIME DP - Unbound Medicine ER -