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Immunocytochemical localization of cannabinoid CB1 receptor and fatty acid amide hydrolase in rat retina.
J Comp Neurol 1999; 415(1):80-90JC

Abstract

Cannabinoids have major effects on central nervous system function. Recent studies indicate that cannabinoid effects on the visual system have a retinal component. Immunocytochemical methods were used to localize cannabinoid CB1 receptor immunoreactivity (CB1R-IR) and an endocannabinoid (anandamide and 2-arachidonylglycerol) degradative enzyme, fatty acid amide hydrolase (FAAH)-IR, in the rat retina. Double labeling with neuron-specific markers permitted identification of cells that were labeled with CB1R-IR and FAAH-IR. CB1R-IR was observed in all cells that were protein kinase C-immunoreactive (rod bipolar cells and a subtype of GABA-amacrine cell) as well as horizontal cells (identified by calbindin-IR). There was also punctate CB1R-IR in the distal one-third of the inner plexiform layer (IPL) that could not be assigned to a cell type. FAAH-IR was most prominent in large ganglion cells, whose dendrites projected to a narrow band in the proximal IPL. Weaker FAAH-IR was observed in the soma of horizontal cells (identified by calbindin-IR); the soma of large, but not small, dopamine amacrine cells (identified by tyrosine hydroxylase-IR); and dendrites of orthotopic- and displaced-starburst amacrine cells (identified by choline acetyltransferase-IR) but in less than 50% of the starburst amacrine cell somata. The extensive distribution of CB1R-IR on horizontal cells and rod bipolar cells indicates a role of endocannabinoids in scotopic vision, whereas the more widespread distribution of FAAH-IR indicates a complex control of endocannabinoid release and degradation in the retina.

Authors+Show Affiliations

Department of Neurobiology, University at Stony Brook, Stony Brook, New York 11794-5230, USA. yazulla@life.bio.sunysb.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10540359

Citation

Yazulla, S, et al. "Immunocytochemical Localization of Cannabinoid CB1 Receptor and Fatty Acid Amide Hydrolase in Rat Retina." The Journal of Comparative Neurology, vol. 415, no. 1, 1999, pp. 80-90.
Yazulla S, Studholme KM, McIntosh HH, et al. Immunocytochemical localization of cannabinoid CB1 receptor and fatty acid amide hydrolase in rat retina. J Comp Neurol. 1999;415(1):80-90.
Yazulla, S., Studholme, K. M., McIntosh, H. H., & Deutsch, D. G. (1999). Immunocytochemical localization of cannabinoid CB1 receptor and fatty acid amide hydrolase in rat retina. The Journal of Comparative Neurology, 415(1), pp. 80-90.
Yazulla S, et al. Immunocytochemical Localization of Cannabinoid CB1 Receptor and Fatty Acid Amide Hydrolase in Rat Retina. J Comp Neurol. 1999 Dec 6;415(1):80-90. PubMed PMID: 10540359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunocytochemical localization of cannabinoid CB1 receptor and fatty acid amide hydrolase in rat retina. AU - Yazulla,S, AU - Studholme,K M, AU - McIntosh,H H, AU - Deutsch,D G, PY - 1999/12/14/pubmed PY - 1999/12/14/medline PY - 1999/12/14/entrez SP - 80 EP - 90 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 415 IS - 1 N2 - Cannabinoids have major effects on central nervous system function. Recent studies indicate that cannabinoid effects on the visual system have a retinal component. Immunocytochemical methods were used to localize cannabinoid CB1 receptor immunoreactivity (CB1R-IR) and an endocannabinoid (anandamide and 2-arachidonylglycerol) degradative enzyme, fatty acid amide hydrolase (FAAH)-IR, in the rat retina. Double labeling with neuron-specific markers permitted identification of cells that were labeled with CB1R-IR and FAAH-IR. CB1R-IR was observed in all cells that were protein kinase C-immunoreactive (rod bipolar cells and a subtype of GABA-amacrine cell) as well as horizontal cells (identified by calbindin-IR). There was also punctate CB1R-IR in the distal one-third of the inner plexiform layer (IPL) that could not be assigned to a cell type. FAAH-IR was most prominent in large ganglion cells, whose dendrites projected to a narrow band in the proximal IPL. Weaker FAAH-IR was observed in the soma of horizontal cells (identified by calbindin-IR); the soma of large, but not small, dopamine amacrine cells (identified by tyrosine hydroxylase-IR); and dendrites of orthotopic- and displaced-starburst amacrine cells (identified by choline acetyltransferase-IR) but in less than 50% of the starburst amacrine cell somata. The extensive distribution of CB1R-IR on horizontal cells and rod bipolar cells indicates a role of endocannabinoids in scotopic vision, whereas the more widespread distribution of FAAH-IR indicates a complex control of endocannabinoid release and degradation in the retina. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/10540359/Immunocytochemical_localization_of_cannabinoid_CB1_receptor_and_fatty_acid_amide_hydrolase_in_rat_retina_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1999&volume=415&issue=1&spage=80 DB - PRIME DP - Unbound Medicine ER -