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A novel allele of RAD52 that causes severe DNA repair and recombination deficiencies only in the absence of RAD51 or RAD59.
Genetics. 1999 Nov; 153(3):1117-30.G

Abstract

With the use of an intrachromosomal inverted repeat as a recombination reporter, we have shown that mitotic recombination is dependent on the RAD52 gene, but reduced only fivefold by mutation of RAD51. RAD59, a component of the RAD51-independent pathway, was identified previously by screening for mutations that reduced inverted-repeat recombination in a rad51 strain. Here we describe a rad52 mutation, rad52R70K, that also reduced recombination synergistically in a rad51 background. The phenotype of the rad52R70K strain, which includes weak gamma-ray sensitivity, a fourfold reduction in the rate of inverted-repeat recombination, elevated allelic recombination, sporulation proficiency, and a reduction in the efficiency of mating-type switching and single-strand annealing, was similar to that observed for deletion of the RAD59 gene. However, rad52R70K rad59 double mutants showed synergistic defects in ionizing radiation resistance, sporulation, and mating-type switching. These results suggest that Rad52 and Rad59 have partially overlapping functions and that Rad59 can substitute for this function of Rad52 in a RAD51 rad52R70K strain.

Authors+Show Affiliations

Department of Microbiology and Institute of Cancer Research, Columbia University, New York, New York 10032, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10545446

Citation

Bai, Y, et al. "A Novel Allele of RAD52 That Causes Severe DNA Repair and Recombination Deficiencies Only in the Absence of RAD51 or RAD59." Genetics, vol. 153, no. 3, 1999, pp. 1117-30.
Bai Y, Davis AP, Symington LS. A novel allele of RAD52 that causes severe DNA repair and recombination deficiencies only in the absence of RAD51 or RAD59. Genetics. 1999;153(3):1117-30.
Bai, Y., Davis, A. P., & Symington, L. S. (1999). A novel allele of RAD52 that causes severe DNA repair and recombination deficiencies only in the absence of RAD51 or RAD59. Genetics, 153(3), 1117-30.
Bai Y, Davis AP, Symington LS. A Novel Allele of RAD52 That Causes Severe DNA Repair and Recombination Deficiencies Only in the Absence of RAD51 or RAD59. Genetics. 1999;153(3):1117-30. PubMed PMID: 10545446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel allele of RAD52 that causes severe DNA repair and recombination deficiencies only in the absence of RAD51 or RAD59. AU - Bai,Y, AU - Davis,A P, AU - Symington,L S, PY - 1999/11/5/pubmed PY - 1999/11/5/medline PY - 1999/11/5/entrez SP - 1117 EP - 30 JF - Genetics JO - Genetics VL - 153 IS - 3 N2 - With the use of an intrachromosomal inverted repeat as a recombination reporter, we have shown that mitotic recombination is dependent on the RAD52 gene, but reduced only fivefold by mutation of RAD51. RAD59, a component of the RAD51-independent pathway, was identified previously by screening for mutations that reduced inverted-repeat recombination in a rad51 strain. Here we describe a rad52 mutation, rad52R70K, that also reduced recombination synergistically in a rad51 background. The phenotype of the rad52R70K strain, which includes weak gamma-ray sensitivity, a fourfold reduction in the rate of inverted-repeat recombination, elevated allelic recombination, sporulation proficiency, and a reduction in the efficiency of mating-type switching and single-strand annealing, was similar to that observed for deletion of the RAD59 gene. However, rad52R70K rad59 double mutants showed synergistic defects in ionizing radiation resistance, sporulation, and mating-type switching. These results suggest that Rad52 and Rad59 have partially overlapping functions and that Rad59 can substitute for this function of Rad52 in a RAD51 rad52R70K strain. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/10545446/A_novel_allele_of_RAD52_that_causes_severe_DNA_repair_and_recombination_deficiencies_only_in_the_absence_of_RAD51_or_RAD59_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1093/genetics/153.3.1117 DB - PRIME DP - Unbound Medicine ER -