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Temporal, spatial, and cell type-specific control of Cre-mediated DNA recombination in transgenic mice.
Nat Biotechnol. 1999 Nov; 17(11):1091-6.NBio

Abstract

We have developed a universal system for temporal, spatial, and cell type-specific control of gene expression in mice that (1) integrates the advantages of tetracycline-controlled gene expression and Cre-recombinase-loxP site-mediated gene inactivation, and (2) simplifies schemes of animal crosses by combination of two control elements in a single transgene. Two transgenic strains were generated in which the cell type-specific control was provided by either the retinoblastoma gene promoter or the whey acidic protein promoter. Both promoters drive the expression of the reverse tetracycline-controlled transactivator (rtTA). Placed in cis configuration to the rtTA transcription unit, the rtTA-inducible promoter directs expression of Cre recombinase. In both strains crossed with cActXstopXLacZ reporter mice, which have a loxP-stop of transcription/translation-loxP-LacZ cassette driven by chicken beta-actin promoter, Cre-loxP-mediated DNA recombination leading to LacZ expression was accurately regulated in a temporal, spatial, and cell type-specific manner. This approach can be applied to establishment of analogous mouse strains with virtually any promoter as systems to control gene regulation in a variety of cell types.

Authors+Show Affiliations

Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, 15355 Lambda Dr., San Antonio, TX 78245-3207, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10545915

Citation

Utomo, A R., et al. "Temporal, Spatial, and Cell Type-specific Control of Cre-mediated DNA Recombination in Transgenic Mice." Nature Biotechnology, vol. 17, no. 11, 1999, pp. 1091-6.
Utomo AR, Nikitin AY, Lee WH. Temporal, spatial, and cell type-specific control of Cre-mediated DNA recombination in transgenic mice. Nat Biotechnol. 1999;17(11):1091-6.
Utomo, A. R., Nikitin, A. Y., & Lee, W. H. (1999). Temporal, spatial, and cell type-specific control of Cre-mediated DNA recombination in transgenic mice. Nature Biotechnology, 17(11), 1091-6.
Utomo AR, Nikitin AY, Lee WH. Temporal, Spatial, and Cell Type-specific Control of Cre-mediated DNA Recombination in Transgenic Mice. Nat Biotechnol. 1999;17(11):1091-6. PubMed PMID: 10545915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal, spatial, and cell type-specific control of Cre-mediated DNA recombination in transgenic mice. AU - Utomo,A R, AU - Nikitin,A Y, AU - Lee,W H, PY - 1999/11/5/pubmed PY - 2001/3/23/medline PY - 1999/11/5/entrez SP - 1091 EP - 6 JF - Nature biotechnology JO - Nat Biotechnol VL - 17 IS - 11 N2 - We have developed a universal system for temporal, spatial, and cell type-specific control of gene expression in mice that (1) integrates the advantages of tetracycline-controlled gene expression and Cre-recombinase-loxP site-mediated gene inactivation, and (2) simplifies schemes of animal crosses by combination of two control elements in a single transgene. Two transgenic strains were generated in which the cell type-specific control was provided by either the retinoblastoma gene promoter or the whey acidic protein promoter. Both promoters drive the expression of the reverse tetracycline-controlled transactivator (rtTA). Placed in cis configuration to the rtTA transcription unit, the rtTA-inducible promoter directs expression of Cre recombinase. In both strains crossed with cActXstopXLacZ reporter mice, which have a loxP-stop of transcription/translation-loxP-LacZ cassette driven by chicken beta-actin promoter, Cre-loxP-mediated DNA recombination leading to LacZ expression was accurately regulated in a temporal, spatial, and cell type-specific manner. This approach can be applied to establishment of analogous mouse strains with virtually any promoter as systems to control gene regulation in a variety of cell types. SN - 1087-0156 UR - https://www.unboundmedicine.com/medline/citation/10545915/Temporal_spatial_and_cell_type_specific_control_of_Cre_mediated_DNA_recombination_in_transgenic_mice_ L2 - https://doi.org/10.1038/15073 DB - PRIME DP - Unbound Medicine ER -