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Partial characterization of polyphenol oxidase activity in raspberry fruits.
J Agric Food Chem 1999; 47(10):4068-72JA

Abstract

A partial characterization of polyphenol oxidase (PPO) activity in raspberry fruits is described. Two early cultivars harvested in May/June (Heritage and Autumm Bliss) and two late cultivars harvested in October-November (Ceva and Rubi) were analyzed for PPO activity. Stable and highly active PPO extracts were obtained using insoluble poly(vinylpyrrolidone) (PVP) and Triton X-100 in sodium phosphate, pH 7.0 buffer. Polyacrylamide gel electrophoresis of raspberry extracts under nondenaturing conditions resolved in one band (R(f)()(1) = 0.25). Raspberry PPO activity has pH optima of 8.0 and 5.5, both with catechol (0.1 M). Maximum activity was with D-catechin (catecholase activity), followed by p-coumaric acid (cresolase activity). Heritage raspberry also showed PPO activity toward 4-methylcatechol. Ceva and Autumm Bliss raspberries showed the higher PPO activity using catechol as substrate.

Authors+Show Affiliations

Plant Foods Science & Technology Department, Instituto del Frío (CSIC), Ciudad Universitaria, 28040-Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10552767

Citation

González, E M., et al. "Partial Characterization of Polyphenol Oxidase Activity in Raspberry Fruits." Journal of Agricultural and Food Chemistry, vol. 47, no. 10, 1999, pp. 4068-72.
González EM, de Ancos B, Cano MP. Partial characterization of polyphenol oxidase activity in raspberry fruits. J Agric Food Chem. 1999;47(10):4068-72.
González, E. M., de Ancos, B., & Cano, M. P. (1999). Partial characterization of polyphenol oxidase activity in raspberry fruits. Journal of Agricultural and Food Chemistry, 47(10), pp. 4068-72.
González EM, de Ancos B, Cano MP. Partial Characterization of Polyphenol Oxidase Activity in Raspberry Fruits. J Agric Food Chem. 1999;47(10):4068-72. PubMed PMID: 10552767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Partial characterization of polyphenol oxidase activity in raspberry fruits. AU - González,E M, AU - de Ancos,B, AU - Cano,M P, PY - 1999/12/10/pubmed PY - 2000/2/19/medline PY - 1999/12/10/entrez SP - 4068 EP - 72 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 47 IS - 10 N2 - A partial characterization of polyphenol oxidase (PPO) activity in raspberry fruits is described. Two early cultivars harvested in May/June (Heritage and Autumm Bliss) and two late cultivars harvested in October-November (Ceva and Rubi) were analyzed for PPO activity. Stable and highly active PPO extracts were obtained using insoluble poly(vinylpyrrolidone) (PVP) and Triton X-100 in sodium phosphate, pH 7.0 buffer. Polyacrylamide gel electrophoresis of raspberry extracts under nondenaturing conditions resolved in one band (R(f)()(1) = 0.25). Raspberry PPO activity has pH optima of 8.0 and 5.5, both with catechol (0.1 M). Maximum activity was with D-catechin (catecholase activity), followed by p-coumaric acid (cresolase activity). Heritage raspberry also showed PPO activity toward 4-methylcatechol. Ceva and Autumm Bliss raspberries showed the higher PPO activity using catechol as substrate. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/10552767/Partial_characterization_of_polyphenol_oxidase_activity_in_raspberry_fruits_ L2 - https://dx.doi.org/10.1021/jf981325q DB - PRIME DP - Unbound Medicine ER -