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Immunological identification of an inward rectifier K+ channel (Kir4.1) in the intermediate cell (melanocyte) of the cochlear stria vascularis of gerbils and rats.
Cell Tissue Res. 1999 Oct; 298(1):179-83.CT

Abstract

The cochlear stria vascularis produces the positive endocochlear potential (EP) and the endolymph. Both the EP and the endolymph are essential for the physiological function of hair cells. The intermediate cell is one of several cell types constituting the stria vascularis. It is known that inward rectifier K+ channels can play a constitutive role in the determination of the resting membrane potential. Localization of a member of the inward rectifier K+ channel family, Kir4.1, in the stria vascularis of gerbils and rats was investigated by immunological methods. A polyclonal antibody specific to the C-terminus of the rat Kir4.1 channel was raised in rabbits. Immunostaining of dissociated cells revealed that the Kir4.1 channel was localized to the intermediate cell, but not to the epithelial marginal cell. Subcellular localization of the Kir4.1 channel to the plasma membrane of the intermediate cell was confirmed by immunoelectron microscopy. Immunostaining of whole-tissue preparations revealed a network-like structure composed of intermediate cells. It seems likely that the Kir4.1 channel mediates the inwardly rectifying K+ current in the intermediate cell as shown previously by electrophysiological methods, and that this channel plays key roles in the production of the EP and K+ transport in the stria vascularis.

Authors+Show Affiliations

Department of Physiology, Kochi Medical School, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10555552

Citation

Ando, M, and S Takeuchi. "Immunological Identification of an Inward Rectifier K+ Channel (Kir4.1) in the Intermediate Cell (melanocyte) of the Cochlear Stria Vascularis of Gerbils and Rats." Cell and Tissue Research, vol. 298, no. 1, 1999, pp. 179-83.
Ando M, Takeuchi S. Immunological identification of an inward rectifier K+ channel (Kir4.1) in the intermediate cell (melanocyte) of the cochlear stria vascularis of gerbils and rats. Cell Tissue Res. 1999;298(1):179-83.
Ando, M., & Takeuchi, S. (1999). Immunological identification of an inward rectifier K+ channel (Kir4.1) in the intermediate cell (melanocyte) of the cochlear stria vascularis of gerbils and rats. Cell and Tissue Research, 298(1), 179-83.
Ando M, Takeuchi S. Immunological Identification of an Inward Rectifier K+ Channel (Kir4.1) in the Intermediate Cell (melanocyte) of the Cochlear Stria Vascularis of Gerbils and Rats. Cell Tissue Res. 1999;298(1):179-83. PubMed PMID: 10555552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunological identification of an inward rectifier K+ channel (Kir4.1) in the intermediate cell (melanocyte) of the cochlear stria vascularis of gerbils and rats. AU - Ando,M, AU - Takeuchi,S, PY - 1999/11/11/pubmed PY - 1999/11/11/medline PY - 1999/11/11/entrez SP - 179 EP - 83 JF - Cell and tissue research JO - Cell Tissue Res. VL - 298 IS - 1 N2 - The cochlear stria vascularis produces the positive endocochlear potential (EP) and the endolymph. Both the EP and the endolymph are essential for the physiological function of hair cells. The intermediate cell is one of several cell types constituting the stria vascularis. It is known that inward rectifier K+ channels can play a constitutive role in the determination of the resting membrane potential. Localization of a member of the inward rectifier K+ channel family, Kir4.1, in the stria vascularis of gerbils and rats was investigated by immunological methods. A polyclonal antibody specific to the C-terminus of the rat Kir4.1 channel was raised in rabbits. Immunostaining of dissociated cells revealed that the Kir4.1 channel was localized to the intermediate cell, but not to the epithelial marginal cell. Subcellular localization of the Kir4.1 channel to the plasma membrane of the intermediate cell was confirmed by immunoelectron microscopy. Immunostaining of whole-tissue preparations revealed a network-like structure composed of intermediate cells. It seems likely that the Kir4.1 channel mediates the inwardly rectifying K+ current in the intermediate cell as shown previously by electrophysiological methods, and that this channel plays key roles in the production of the EP and K+ transport in the stria vascularis. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/10555552/Immunological_identification_of_an_inward_rectifier_K+_channel__Kir4_1__in_the_intermediate_cell__melanocyte__of_the_cochlear_stria_vascularis_of_gerbils_and_rats_ L2 - https://dx.doi.org/10.1007/s004419900066 DB - PRIME DP - Unbound Medicine ER -