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Peptide 19-immunoreactive primary sensory neurons in the rat trigeminal ganglion.
Brain Res. 1999 Nov 06; 846(2):274-9.BR

Abstract

Peptide 19-immunoreactivity (PEP 19-IR) was examined in the trigeminal ganglion (TG) of the adult rat. A half of TG neurons were immunoreactive(IR) for PEP 19. PEP 19-IR neurons were mostly medium-sized to large. 66% of TG neurons > 600 microm(2) and 38% of those in the range 300-600 microm(2) showed the IR. TG neurons <300 microm(2) were mostly devoid of PEP 19-IR (86%). A double immunofluorescence method revealed the coexpression of PEP 19 and calcium-binding proteins. 31% and 16% of PEP 19-IR neurons exhibited parvalbumin- and calbindin D-28k-IRs, respectively. Conversely, a half of parvalbumin- (53%) and calbindin D-28k-IR (55%) neurons coexpressed PEP 19-IR. PEP 19-IR neurons were mostly IR for S100 (91%) and 80% of S100-IR neurons showed PEP 19-IR. Virtually all (99%) PEP 19-IR neurons were devoid of calcitonin gene-related peptide (CGRP)-IR. The molar tooth pulp contained PEP 19-IR nerve fibers. In the root pulp, PEP 19-IR nerve fibers projected straight until they reached the coronal pulp. Accompanied by blood vessels, these nerve fibers ascended toward the pulp horn. They formed nerve plexuses in the subodontoblastic layer, and reached the base of the odontoblastic layer. However, PEP 19-IR nerve fibers could not be observed within the odontoblastic layer, predentine or dentine. The distribution of these nerve fibers was similar to that of parvalbumin-IR ones. In the TG, PEP 19-IR was found in 34% of primary sensory neurons retrogradely labeled from the molar tooth pulp. 80% of PEP 19-IR tooth pulp TG neurons coexpressed parvalbumin-IR. An immunoelectron microscopic method revealed that a half of radicular axons showed PEP 19-IR. 80% of myelinated axons exhibited PEP 19-IR, whereas 20% of unmyelinated ones showed the IR. In the subodontoblastic layer, PEP 19-IR nerve fibers mostly lost myelin sheath or Schwann cell ensheathment. At the base of the odontoblastic layer, PEP 19-IR neurites made close contact with odontoblasts. PEP 19-IR nerve endings could not be observed in other oro-facial tissues. The coexpression of PEP 19 and CaBPs suggests that low-threshold mechanoreceptors contain PEP 19-IR in the TG. It is also likely that PEP 19-IR TG neurons include myelinated nociceptors.

Authors+Show Affiliations

Second Department of Oral Anatomy, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama, Japan.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10556647

Citation

Ichikawa, H, and T Sugimoto. "Peptide 19-immunoreactive Primary Sensory Neurons in the Rat Trigeminal Ganglion." Brain Research, vol. 846, no. 2, 1999, pp. 274-9.
Ichikawa H, Sugimoto T. Peptide 19-immunoreactive primary sensory neurons in the rat trigeminal ganglion. Brain Res. 1999;846(2):274-9.
Ichikawa, H., & Sugimoto, T. (1999). Peptide 19-immunoreactive primary sensory neurons in the rat trigeminal ganglion. Brain Research, 846(2), 274-9.
Ichikawa H, Sugimoto T. Peptide 19-immunoreactive Primary Sensory Neurons in the Rat Trigeminal Ganglion. Brain Res. 1999 Nov 6;846(2):274-9. PubMed PMID: 10556647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peptide 19-immunoreactive primary sensory neurons in the rat trigeminal ganglion. AU - Ichikawa,H, AU - Sugimoto,T, PY - 1999/11/11/pubmed PY - 1999/11/11/medline PY - 1999/11/11/entrez SP - 274 EP - 9 JF - Brain research JO - Brain Res. VL - 846 IS - 2 N2 - Peptide 19-immunoreactivity (PEP 19-IR) was examined in the trigeminal ganglion (TG) of the adult rat. A half of TG neurons were immunoreactive(IR) for PEP 19. PEP 19-IR neurons were mostly medium-sized to large. 66% of TG neurons > 600 microm(2) and 38% of those in the range 300-600 microm(2) showed the IR. TG neurons <300 microm(2) were mostly devoid of PEP 19-IR (86%). A double immunofluorescence method revealed the coexpression of PEP 19 and calcium-binding proteins. 31% and 16% of PEP 19-IR neurons exhibited parvalbumin- and calbindin D-28k-IRs, respectively. Conversely, a half of parvalbumin- (53%) and calbindin D-28k-IR (55%) neurons coexpressed PEP 19-IR. PEP 19-IR neurons were mostly IR for S100 (91%) and 80% of S100-IR neurons showed PEP 19-IR. Virtually all (99%) PEP 19-IR neurons were devoid of calcitonin gene-related peptide (CGRP)-IR. The molar tooth pulp contained PEP 19-IR nerve fibers. In the root pulp, PEP 19-IR nerve fibers projected straight until they reached the coronal pulp. Accompanied by blood vessels, these nerve fibers ascended toward the pulp horn. They formed nerve plexuses in the subodontoblastic layer, and reached the base of the odontoblastic layer. However, PEP 19-IR nerve fibers could not be observed within the odontoblastic layer, predentine or dentine. The distribution of these nerve fibers was similar to that of parvalbumin-IR ones. In the TG, PEP 19-IR was found in 34% of primary sensory neurons retrogradely labeled from the molar tooth pulp. 80% of PEP 19-IR tooth pulp TG neurons coexpressed parvalbumin-IR. An immunoelectron microscopic method revealed that a half of radicular axons showed PEP 19-IR. 80% of myelinated axons exhibited PEP 19-IR, whereas 20% of unmyelinated ones showed the IR. In the subodontoblastic layer, PEP 19-IR nerve fibers mostly lost myelin sheath or Schwann cell ensheathment. At the base of the odontoblastic layer, PEP 19-IR neurites made close contact with odontoblasts. PEP 19-IR nerve endings could not be observed in other oro-facial tissues. The coexpression of PEP 19 and CaBPs suggests that low-threshold mechanoreceptors contain PEP 19-IR in the TG. It is also likely that PEP 19-IR TG neurons include myelinated nociceptors. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/10556647/Peptide_19_immunoreactive_primary_sensory_neurons_in_the_rat_trigeminal_ganglion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(99)02014-4 DB - PRIME DP - Unbound Medicine ER -