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Bax membrane insertion during Fas(CD95)-induced apoptosis precedes cytochrome c release and is inhibited by Bcl-2.
Oncogene. 1999 Oct 28; 18(44):5991-9.O

Abstract

Ligation of the Fas cell surface receptor leads to activation of caspases and subsequent apoptosis. Members of the Bcl-2 family of proteins control the cellular commitment to apoptosis, although their role in Fas-induced apoptosis is ill-defined. In this report we demonstrate that the pro-apoptotic protein, Bax, translocates from the cytosol specifically to the mitochondria following Fas ligation in MCF10A1 breast epithelial cells. Bax translocation was dependent on caspase activation, and preceded the release of cytochrome c and loss of mitochondrial respiratory activity. Bax translocation occurred in concert with activation of downstream caspases as determined by cleavage of a synthetic substrate, proteolysis of poly(ADP-ribose) polymerase, and processing of procaspase-3 and -7. Overexpression of the anti-apoptotic protein, Bcl-2, prevented Bax insertion, cytochrome c release, complete processing of caspase-3 and -7, and full activation of DEVD-specific cleavage activity. These data establish a role for Bax mitochondrial insertion during Fas-mediated apoptosis, and support a model in which Bax insertion amplifies the Fas apoptotic cascade through cytochrome c release and complete processing of caspases-3 and -7. In addition, our findings indicate that prevention of Bax insertion into the mitochondria represents a novel mechanism by which Bcl-2 inhibits Fas-induced apoptosis.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of Louisville, Louisville, Kentucky, KY 40292, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10557088

Citation

Murphy, K M., et al. "Bax Membrane Insertion During Fas(CD95)-induced Apoptosis Precedes Cytochrome C Release and Is Inhibited By Bcl-2." Oncogene, vol. 18, no. 44, 1999, pp. 5991-9.
Murphy KM, Streips UN, Lock RB. Bax membrane insertion during Fas(CD95)-induced apoptosis precedes cytochrome c release and is inhibited by Bcl-2. Oncogene. 1999;18(44):5991-9.
Murphy, K. M., Streips, U. N., & Lock, R. B. (1999). Bax membrane insertion during Fas(CD95)-induced apoptosis precedes cytochrome c release and is inhibited by Bcl-2. Oncogene, 18(44), 5991-9.
Murphy KM, Streips UN, Lock RB. Bax Membrane Insertion During Fas(CD95)-induced Apoptosis Precedes Cytochrome C Release and Is Inhibited By Bcl-2. Oncogene. 1999 Oct 28;18(44):5991-9. PubMed PMID: 10557088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bax membrane insertion during Fas(CD95)-induced apoptosis precedes cytochrome c release and is inhibited by Bcl-2. AU - Murphy,K M, AU - Streips,U N, AU - Lock,R B, PY - 1999/11/11/pubmed PY - 1999/11/11/medline PY - 1999/11/11/entrez SP - 5991 EP - 9 JF - Oncogene JO - Oncogene VL - 18 IS - 44 N2 - Ligation of the Fas cell surface receptor leads to activation of caspases and subsequent apoptosis. Members of the Bcl-2 family of proteins control the cellular commitment to apoptosis, although their role in Fas-induced apoptosis is ill-defined. In this report we demonstrate that the pro-apoptotic protein, Bax, translocates from the cytosol specifically to the mitochondria following Fas ligation in MCF10A1 breast epithelial cells. Bax translocation was dependent on caspase activation, and preceded the release of cytochrome c and loss of mitochondrial respiratory activity. Bax translocation occurred in concert with activation of downstream caspases as determined by cleavage of a synthetic substrate, proteolysis of poly(ADP-ribose) polymerase, and processing of procaspase-3 and -7. Overexpression of the anti-apoptotic protein, Bcl-2, prevented Bax insertion, cytochrome c release, complete processing of caspase-3 and -7, and full activation of DEVD-specific cleavage activity. These data establish a role for Bax mitochondrial insertion during Fas-mediated apoptosis, and support a model in which Bax insertion amplifies the Fas apoptotic cascade through cytochrome c release and complete processing of caspases-3 and -7. In addition, our findings indicate that prevention of Bax insertion into the mitochondria represents a novel mechanism by which Bcl-2 inhibits Fas-induced apoptosis. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/10557088/Bax_membrane_insertion_during_Fas_CD95__induced_apoptosis_precedes_cytochrome_c_release_and_is_inhibited_by_Bcl_2_ L2 - https://doi.org/10.1038/sj.onc.1203001 DB - PRIME DP - Unbound Medicine ER -