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Purification, characterization, and cDNA cloning of a new fibrinogenlytic venom protein, Agkisacutacin, from Agkistrodon acutus venom.
Biochem Biophys Res Commun. 1999 Nov 19; 265(2):530-5.BB

Abstract

Agkisacutacin is a new fibrinogenlytic protein from Agkistrodon acutus venom. It consists of two heterologous subunits linked by an intersubunit disulfide bond. The cDNAs encoding the two chains of Agkisacutacin were cloned from a lambdagt11 cDNA library of the snake venom gland and sequenced, including the leader peptides (23/23 amino acid residues) and mature subunits (129/123 amino acid residues). It is structurally related to the family of IX/X-binding protein (IX/X-bp)-like proteins and shows high similarity (alpha-70%/beta-64%) to habu IX/X-bp from Trimeresurus flavoridis, but displays distinct biological activity with direct action on fibrinogen.

Authors+Show Affiliations

School of Life Science, University of Science and Technology of China, Hefei, Anhui, 230027, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10558903

Citation

Cheng, X, et al. "Purification, Characterization, and cDNA Cloning of a New Fibrinogenlytic Venom Protein, Agkisacutacin, From Agkistrodon Acutus Venom." Biochemical and Biophysical Research Communications, vol. 265, no. 2, 1999, pp. 530-5.
Cheng X, Qian Y, Liu Q, et al. Purification, characterization, and cDNA cloning of a new fibrinogenlytic venom protein, Agkisacutacin, from Agkistrodon acutus venom. Biochem Biophys Res Commun. 1999;265(2):530-5.
Cheng, X., Qian, Y., Liu, Q., Li, B. X., Zhang, M., & Liu, J. (1999). Purification, characterization, and cDNA cloning of a new fibrinogenlytic venom protein, Agkisacutacin, from Agkistrodon acutus venom. Biochemical and Biophysical Research Communications, 265(2), 530-5.
Cheng X, et al. Purification, Characterization, and cDNA Cloning of a New Fibrinogenlytic Venom Protein, Agkisacutacin, From Agkistrodon Acutus Venom. Biochem Biophys Res Commun. 1999 Nov 19;265(2):530-5. PubMed PMID: 10558903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification, characterization, and cDNA cloning of a new fibrinogenlytic venom protein, Agkisacutacin, from Agkistrodon acutus venom. AU - Cheng,X, AU - Qian,Y, AU - Liu,Q, AU - Li,B X, AU - Zhang,M, AU - Liu,J, PY - 1999/11/24/pubmed PY - 1999/11/24/medline PY - 1999/11/24/entrez SP - 530 EP - 5 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 265 IS - 2 N2 - Agkisacutacin is a new fibrinogenlytic protein from Agkistrodon acutus venom. It consists of two heterologous subunits linked by an intersubunit disulfide bond. The cDNAs encoding the two chains of Agkisacutacin were cloned from a lambdagt11 cDNA library of the snake venom gland and sequenced, including the leader peptides (23/23 amino acid residues) and mature subunits (129/123 amino acid residues). It is structurally related to the family of IX/X-binding protein (IX/X-bp)-like proteins and shows high similarity (alpha-70%/beta-64%) to habu IX/X-bp from Trimeresurus flavoridis, but displays distinct biological activity with direct action on fibrinogen. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/10558903/Purification_characterization_and_cDNA_cloning_of_a_new_fibrinogenlytic_venom_protein_Agkisacutacin_from_Agkistrodon_acutus_venom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(99)91685-6 DB - PRIME DP - Unbound Medicine ER -