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CTRP is essential for mosquito infection by malaria ookinetes.
EMBO J. 1999 Nov 15; 18(22):6221-7.EJ

Abstract

The malaria parasite suffers severe population losses as it passes through its mosquito vector. Contributing factors are the essential but highly constrained developmental transitions that the parasite undergoes in the mosquito midgut, combined with the invasion of the midgut epithelium by the malaria ookinete (recently described as a principal elicitor of the innate immune response in the Plasmodium-infected insect). Little is known about the molecular organization of these midgut-stage parasites and their critical interactions with the blood meal and the mosquito vector. Elucidation of these molecules and interactions will open up new avenues for chemotherapeutic and immunological attack of parasite development. Here, using the rodent malaria parasite Plasmodium berghei, we identify and characterize the first microneme protein of the ookinete: circumsporozoite- and TRAP-related protein (CTRP). We show that transgenic parasites in which the CTRP gene is disrupted form ookinetes that have reduced motility, fail to invade the midgut epithelium, do not trigger the mosquito immune response, and do not develop further into oocysts. Thus, CTRP is the first molecule shown to be essential for ookinete infectivity and, consequently, mosquito transmission of malaria.

Authors+Show Affiliations

Infection and Immunity Section, Department of Biology, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10562534

Citation

Dessens, J T., et al. "CTRP Is Essential for Mosquito Infection By Malaria Ookinetes." The EMBO Journal, vol. 18, no. 22, 1999, pp. 6221-7.
Dessens JT, Beetsma AL, Dimopoulos G, et al. CTRP is essential for mosquito infection by malaria ookinetes. EMBO J. 1999;18(22):6221-7.
Dessens, J. T., Beetsma, A. L., Dimopoulos, G., Wengelnik, K., Crisanti, A., Kafatos, F. C., & Sinden, R. E. (1999). CTRP is essential for mosquito infection by malaria ookinetes. The EMBO Journal, 18(22), 6221-7.
Dessens JT, et al. CTRP Is Essential for Mosquito Infection By Malaria Ookinetes. EMBO J. 1999 Nov 15;18(22):6221-7. PubMed PMID: 10562534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CTRP is essential for mosquito infection by malaria ookinetes. AU - Dessens,J T, AU - Beetsma,A L, AU - Dimopoulos,G, AU - Wengelnik,K, AU - Crisanti,A, AU - Kafatos,F C, AU - Sinden,R E, PY - 1999/11/24/pubmed PY - 1999/11/24/medline PY - 1999/11/24/entrez SP - 6221 EP - 7 JF - The EMBO journal JO - EMBO J. VL - 18 IS - 22 N2 - The malaria parasite suffers severe population losses as it passes through its mosquito vector. Contributing factors are the essential but highly constrained developmental transitions that the parasite undergoes in the mosquito midgut, combined with the invasion of the midgut epithelium by the malaria ookinete (recently described as a principal elicitor of the innate immune response in the Plasmodium-infected insect). Little is known about the molecular organization of these midgut-stage parasites and their critical interactions with the blood meal and the mosquito vector. Elucidation of these molecules and interactions will open up new avenues for chemotherapeutic and immunological attack of parasite development. Here, using the rodent malaria parasite Plasmodium berghei, we identify and characterize the first microneme protein of the ookinete: circumsporozoite- and TRAP-related protein (CTRP). We show that transgenic parasites in which the CTRP gene is disrupted form ookinetes that have reduced motility, fail to invade the midgut epithelium, do not trigger the mosquito immune response, and do not develop further into oocysts. Thus, CTRP is the first molecule shown to be essential for ookinete infectivity and, consequently, mosquito transmission of malaria. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/10562534/CTRP_is_essential_for_mosquito_infection_by_malaria_ookinetes_ L2 - https://doi.org/10.1093/emboj/18.22.6221 DB - PRIME DP - Unbound Medicine ER -