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Single strand DNA binding and annealing activities in the yeast recombination factor Rad59.
J Biol Chem. 1999 Nov 26; 274(48):33839-42.JB

Abstract

Saccharomyces cerevisiae RAD59 gene is required for homologous recombination processes and normal level of resistance to ionizing radiation. To study the biochemical functions of Rad59, it was overproduced in yeast and purified to near homogeneity. Rad59 binds DNA, showing much higher affinity for ssDNA than dsDNA. Rad59 also anneals complementary DNA strands, and order of addition experiments indicate that maximal annealing efficiency is achieved when both complementary DNA strands are present upon addition of Rad59. Thus, Rad59 resembles its homolog Rad52 in being able to bind ssDNA and anneal complementary DNA strands. However, unlike Rad52, DNA annealing by Rad59 is not accelerated by the ssDNA binding factor RPA. DNA binding and strand annealing are likely to be important for the biological functions of Rad59 in general recombination and in the single-strand annealing pathway of recombination.

Authors+Show Affiliations

Department of Molecular Medicine/Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245-3207, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10567339

Citation

Petukhova, G, et al. "Single Strand DNA Binding and Annealing Activities in the Yeast Recombination Factor Rad59." The Journal of Biological Chemistry, vol. 274, no. 48, 1999, pp. 33839-42.
Petukhova G, Stratton SA, Sung P. Single strand DNA binding and annealing activities in the yeast recombination factor Rad59. J Biol Chem. 1999;274(48):33839-42.
Petukhova, G., Stratton, S. A., & Sung, P. (1999). Single strand DNA binding and annealing activities in the yeast recombination factor Rad59. The Journal of Biological Chemistry, 274(48), 33839-42.
Petukhova G, Stratton SA, Sung P. Single Strand DNA Binding and Annealing Activities in the Yeast Recombination Factor Rad59. J Biol Chem. 1999 Nov 26;274(48):33839-42. PubMed PMID: 10567339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single strand DNA binding and annealing activities in the yeast recombination factor Rad59. AU - Petukhova,G, AU - Stratton,S A, AU - Sung,P, PY - 1999/11/24/pubmed PY - 1999/11/24/medline PY - 1999/11/24/entrez SP - 33839 EP - 42 JF - The Journal of biological chemistry JO - J Biol Chem VL - 274 IS - 48 N2 - Saccharomyces cerevisiae RAD59 gene is required for homologous recombination processes and normal level of resistance to ionizing radiation. To study the biochemical functions of Rad59, it was overproduced in yeast and purified to near homogeneity. Rad59 binds DNA, showing much higher affinity for ssDNA than dsDNA. Rad59 also anneals complementary DNA strands, and order of addition experiments indicate that maximal annealing efficiency is achieved when both complementary DNA strands are present upon addition of Rad59. Thus, Rad59 resembles its homolog Rad52 in being able to bind ssDNA and anneal complementary DNA strands. However, unlike Rad52, DNA annealing by Rad59 is not accelerated by the ssDNA binding factor RPA. DNA binding and strand annealing are likely to be important for the biological functions of Rad59 in general recombination and in the single-strand annealing pathway of recombination. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/10567339/Single_strand_DNA_binding_and_annealing_activities_in_the_yeast_recombination_factor_Rad59_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(19)53466-4 DB - PRIME DP - Unbound Medicine ER -