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Polyhydroxyalkanoate synthesis in transgenic plants as a new tool to study carbon flow through beta-oxidation.
Plant J. 1999 Oct; 20(1):45-55.PJ

Abstract

Transgenic plants producing peroxisomal polyhydroxy- alkanoate (PHA) from intermediates of fatty acid degradation were used to study carbon flow through the beta-oxidation cycle. Growth of transgenic plants in media containing fatty acids conjugated to Tween detergents resulted in an increased accumulation of PHA and incorporation into the polyester of monomers derived from the beta-oxidation of these fatty acids. Tween-laurate was a stronger inducer of beta-oxidation, as measured by acyl-CoA oxidase activity, and a more potent modulator of PHA quantity and monomer composition than Tween-oleate. Plants co-expressing a peroxisomal PHA synthase with a capryl-acyl carrier protein thioesterase from Cuphea lanceolata produced eightfold more PHA compared to plants expressing only the PHA synthase. PHA produced in double transgenic plants contained mainly saturated monomers ranging from 6 to 10 carbons, indicating an enhanced flow of capric acid towards beta-oxidation. Together, these results support the hypothesis that plant cells have mechanisms which sense levels of free or esterified unusual fatty acids, resulting in changes in the activity of the beta-oxidation cycle as well as removal and degradation of these unusual fatty acids through beta-oxidation. Such enhanced flow of fatty acids through beta-oxidation can be utilized to modulate the amount and composition of PHA produced in transgenic plants. Furthermore, synthesis of PHAs in plants can be used as a new tool to study the quality and relative quantity of the carbon flow through beta-oxidation as well as to analyse the degradation pathway of unusual fatty acids.

Authors+Show Affiliations

Institut dEcologie-Biologie et Physiologie Végétales, Université de Lausanne, CH-1015, Lausanne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10571864

Citation

Mittendorf, V, et al. "Polyhydroxyalkanoate Synthesis in Transgenic Plants as a New Tool to Study Carbon Flow Through Beta-oxidation." The Plant Journal : for Cell and Molecular Biology, vol. 20, no. 1, 1999, pp. 45-55.
Mittendorf V, Bongcam V, Allenbach L, et al. Polyhydroxyalkanoate synthesis in transgenic plants as a new tool to study carbon flow through beta-oxidation. Plant J. 1999;20(1):45-55.
Mittendorf, V., Bongcam, V., Allenbach, L., Coullerez, G., Martini, N., & Poirier, Y. (1999). Polyhydroxyalkanoate synthesis in transgenic plants as a new tool to study carbon flow through beta-oxidation. The Plant Journal : for Cell and Molecular Biology, 20(1), 45-55.
Mittendorf V, et al. Polyhydroxyalkanoate Synthesis in Transgenic Plants as a New Tool to Study Carbon Flow Through Beta-oxidation. Plant J. 1999;20(1):45-55. PubMed PMID: 10571864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyhydroxyalkanoate synthesis in transgenic plants as a new tool to study carbon flow through beta-oxidation. AU - Mittendorf,V, AU - Bongcam,V, AU - Allenbach,L, AU - Coullerez,G, AU - Martini,N, AU - Poirier,Y, PY - 1999/11/26/pubmed PY - 1999/11/26/medline PY - 1999/11/26/entrez SP - 45 EP - 55 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 20 IS - 1 N2 - Transgenic plants producing peroxisomal polyhydroxy- alkanoate (PHA) from intermediates of fatty acid degradation were used to study carbon flow through the beta-oxidation cycle. Growth of transgenic plants in media containing fatty acids conjugated to Tween detergents resulted in an increased accumulation of PHA and incorporation into the polyester of monomers derived from the beta-oxidation of these fatty acids. Tween-laurate was a stronger inducer of beta-oxidation, as measured by acyl-CoA oxidase activity, and a more potent modulator of PHA quantity and monomer composition than Tween-oleate. Plants co-expressing a peroxisomal PHA synthase with a capryl-acyl carrier protein thioesterase from Cuphea lanceolata produced eightfold more PHA compared to plants expressing only the PHA synthase. PHA produced in double transgenic plants contained mainly saturated monomers ranging from 6 to 10 carbons, indicating an enhanced flow of capric acid towards beta-oxidation. Together, these results support the hypothesis that plant cells have mechanisms which sense levels of free or esterified unusual fatty acids, resulting in changes in the activity of the beta-oxidation cycle as well as removal and degradation of these unusual fatty acids through beta-oxidation. Such enhanced flow of fatty acids through beta-oxidation can be utilized to modulate the amount and composition of PHA produced in transgenic plants. Furthermore, synthesis of PHAs in plants can be used as a new tool to study the quality and relative quantity of the carbon flow through beta-oxidation as well as to analyse the degradation pathway of unusual fatty acids. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/10571864/Polyhydroxyalkanoate_synthesis_in_transgenic_plants_as_a_new_tool_to_study_carbon_flow_through_beta_oxidation_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0960-7412&date=1999&volume=20&issue=1&spage=45 DB - PRIME DP - Unbound Medicine ER -