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Analysis of methanol or formic acid in body fluids by headspace solid-phase microextraction and capillary gas chromatography.
J Chromatogr B Biomed Sci Appl. 1999 Oct 29; 734(1):155-62.JC

Abstract

Methanol and its metabolite formic acid have been found extractable from human whole blood and urine by headspace solid-phase microextraction (SPME) with a Carboxen/polydimethylsiloxane fiber. The headspace SPME for formic acid was carried out after derivatization to methyl formate under acidic conditions. The determinations of both compounds were made by using acetonitrile as internal standard (IS) and capillary gas chromatography (GC) with flame ionization detection. The headspace SPME-GC gave sharp peaks for methanol, methyl formate and I.S.; and low background noises for whole blood and urine samples. Extraction efficiencies were 0.25-1.05% of methanol and 0.38-0.84% formic acid for whole blood and urine. The calibration curves for methanol and formic acid showed excellent linearity in the range of 1.56 to 800 and 1.56 to 500 microg/0.5 ml of whole blood or urine, respectively. The detection limits were 0.1-0.5 microg/0.5 ml for methanol and 0.6 microg/0.5 ml for formic acid for both body fluids. The within-day relative standard deviations in terms of extraction efficiency for both compounds in whole blood and urine samples were not greater than 9.8%. By using the established SPME method, methanol and formic acid were successfully separated and determined in rat blood after oral administration of methanol.

Authors+Show Affiliations

Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10574201

Citation

Lee, X P., et al. "Analysis of Methanol or Formic Acid in Body Fluids By Headspace Solid-phase Microextraction and Capillary Gas Chromatography." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 734, no. 1, 1999, pp. 155-62.
Lee XP, Kumazawa T, Kondo K, et al. Analysis of methanol or formic acid in body fluids by headspace solid-phase microextraction and capillary gas chromatography. J Chromatogr B Biomed Sci Appl. 1999;734(1):155-62.
Lee, X. P., Kumazawa, T., Kondo, K., Sato, K., & Suzuki, O. (1999). Analysis of methanol or formic acid in body fluids by headspace solid-phase microextraction and capillary gas chromatography. Journal of Chromatography. B, Biomedical Sciences and Applications, 734(1), 155-62.
Lee XP, et al. Analysis of Methanol or Formic Acid in Body Fluids By Headspace Solid-phase Microextraction and Capillary Gas Chromatography. J Chromatogr B Biomed Sci Appl. 1999 Oct 29;734(1):155-62. PubMed PMID: 10574201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of methanol or formic acid in body fluids by headspace solid-phase microextraction and capillary gas chromatography. AU - Lee,X P, AU - Kumazawa,T, AU - Kondo,K, AU - Sato,K, AU - Suzuki,O, PY - 1999/11/26/pubmed PY - 1999/11/26/medline PY - 1999/11/26/entrez SP - 155 EP - 62 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 734 IS - 1 N2 - Methanol and its metabolite formic acid have been found extractable from human whole blood and urine by headspace solid-phase microextraction (SPME) with a Carboxen/polydimethylsiloxane fiber. The headspace SPME for formic acid was carried out after derivatization to methyl formate under acidic conditions. The determinations of both compounds were made by using acetonitrile as internal standard (IS) and capillary gas chromatography (GC) with flame ionization detection. The headspace SPME-GC gave sharp peaks for methanol, methyl formate and I.S.; and low background noises for whole blood and urine samples. Extraction efficiencies were 0.25-1.05% of methanol and 0.38-0.84% formic acid for whole blood and urine. The calibration curves for methanol and formic acid showed excellent linearity in the range of 1.56 to 800 and 1.56 to 500 microg/0.5 ml of whole blood or urine, respectively. The detection limits were 0.1-0.5 microg/0.5 ml for methanol and 0.6 microg/0.5 ml for formic acid for both body fluids. The within-day relative standard deviations in terms of extraction efficiency for both compounds in whole blood and urine samples were not greater than 9.8%. By using the established SPME method, methanol and formic acid were successfully separated and determined in rat blood after oral administration of methanol. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/10574201/Analysis_of_methanol_or_formic_acid_in_body_fluids_by_headspace_solid_phase_microextraction_and_capillary_gas_chromatography_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:10574201 DB - PRIME DP - Unbound Medicine ER -