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Large-scale analysis of the yeast genome by transposon tagging and gene disruption.
Nature. 1999 Nov 25; 402(6760):413-8.Nat

Abstract

Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest collection of defined yeast mutants ever generated within a single genetic background--a collection of over 11,000 strains, each carrying a transposon inserted within a region of the genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third of the 6,200 predicted genes in the yeast genome. We have used this collection to determine disruption phenotypes for nearly 8,000 strains using 20 different growth conditions; the resulting data sets were clustered to identify groups of functionally related genes. We have also identified over 300 previously non-annotated open reading frames and analysed by indirect immunofluorescence over 1,300 transposon-tagged proteins. In total, our study encompasses over 260,000 data points, constituting the largest functional analysis of the yeast genome ever undertaken.

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Authors+Show Affiliations

Ross-Macdonald P
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Coelho PS
No affiliation info available
Roemer T
No affiliation info available
Agarwal S
No affiliation info available
Kumar A
No affiliation info available
Jansen R
No affiliation info available
Cheung KH
No affiliation info available
Sheehan A
No affiliation info available
Symoniatis D
No affiliation info available
Umansky L
No affiliation info available
Heidtman M
No affiliation info available
Nelson FK
No affiliation info available
Iwasaki H
No affiliation info available
Hager K
No affiliation info available
Gerstein M
No affiliation info available
Miller P
No affiliation info available
Roeder GS
No affiliation info available
Snyder M
No affiliation info available

MeSH

AlgorithmsDNA Transposable ElementsEscherichia coliFungal ProteinsGene Expression ProfilingGenetic TechniquesGenome, FungalMolecular Sequence DataMutagenesisOligonucleotide Array Sequence AnalysisOpen Reading FramesPhenotypePolymerase Chain ReactionSaccharomyces cerevisiaeTransformation, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10586881

Citation

Ross-Macdonald, P, et al. "Large-scale Analysis of the Yeast Genome By Transposon Tagging and Gene Disruption." Nature, vol. 402, no. 6760, 1999, pp. 413-8.
Ross-Macdonald P, Coelho PS, Roemer T, et al. Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature. 1999;402(6760):413-8.
Ross-Macdonald, P., Coelho, P. S., Roemer, T., Agarwal, S., Kumar, A., Jansen, R., Cheung, K. H., Sheehan, A., Symoniatis, D., Umansky, L., Heidtman, M., Nelson, F. K., Iwasaki, H., Hager, K., Gerstein, M., Miller, P., Roeder, G. S., & Snyder, M. (1999). Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature, 402(6760), 413-8.
Ross-Macdonald P, et al. Large-scale Analysis of the Yeast Genome By Transposon Tagging and Gene Disruption. Nature. 1999 Nov 25;402(6760):413-8. PubMed PMID: 10586881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large-scale analysis of the yeast genome by transposon tagging and gene disruption. AU - Ross-Macdonald,P, AU - Coelho,P S, AU - Roemer,T, AU - Agarwal,S, AU - Kumar,A, AU - Jansen,R, AU - Cheung,K H, AU - Sheehan,A, AU - Symoniatis,D, AU - Umansky,L, AU - Heidtman,M, AU - Nelson,F K, AU - Iwasaki,H, AU - Hager,K, AU - Gerstein,M, AU - Miller,P, AU - Roeder,G S, AU - Snyder,M, PY - 1999/12/10/pubmed PY - 2001/3/23/medline PY - 1999/12/10/entrez SP - 413 EP - 8 JF - Nature JO - Nature VL - 402 IS - 6760 N2 - Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest collection of defined yeast mutants ever generated within a single genetic background--a collection of over 11,000 strains, each carrying a transposon inserted within a region of the genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third of the 6,200 predicted genes in the yeast genome. We have used this collection to determine disruption phenotypes for nearly 8,000 strains using 20 different growth conditions; the resulting data sets were clustered to identify groups of functionally related genes. We have also identified over 300 previously non-annotated open reading frames and analysed by indirect immunofluorescence over 1,300 transposon-tagged proteins. In total, our study encompasses over 260,000 data points, constituting the largest functional analysis of the yeast genome ever undertaken. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/10586881/Large_scale_analysis_of_the_yeast_genome_by_transposon_tagging_and_gene_disruption_ L2 - https://doi.org/10.1038/46558 DB - PRIME DP - Unbound Medicine ER -