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Sequence analysis of three Bacillus cereus loci carrying PIcR-regulated genes encoding degradative enzymes and enterotoxin.
Microbiology. 1999 Nov; 145 (Pt 11):3129-3138.M

Abstract

PIcR is a pleiotropic regulator of extracellular virulence factors in the opportunistic human pathogen Bacillus cereus and the entomopathogenic Bacillus thuringiensis, and is induced in cells entering stationary phase. Among the genes regulated by PIcR are: pIcA, encoding phosphatidylinositol-specific phospholipase C (PI-PLC); plc, encoding phosphatidylcholine-preferring phospholipase C (PC-PLC); nhe, encoding the non-haemolytic enterotoxin; hbl, encoding haemolytic enterotoxin BL (HBL); and genes specifying a putative S-layer like surface protein and a putative extracellular RNase. By analysing 37.1 kb of DNA sequence surrounding hbl, plcA and plcR, 28 ORFs were predicted. Three novel genes putatively regulated by PlcR and encoding a neutral protease (NprB), a subtilase family serine protease (Sfp) and a putative cell-wall hydrolase (Cwh) were identified. The corresponding sfp and cwh genes were located in the immediate upstream region of plcA and could both be regulated by a putative PlcR-binding site positioned between the inversely transcribed genes. Similarly, nprB was positioned directly upstream and transcribed in the opposite orientation to plcR. Genes surrounding plcA, plcR and hblCDAB that were lacking an upstream PlcR regulatory sequence did not appear to serve functions apparently related to PlcR and did not exhibit a conserved organization in Bacillus subtilis.

Authors+Show Affiliations

Biotechnology Centre of Oslo and School of Pharmacy, University of Oslo, PO Box 1123, N-0349 Oslo, Norway1.Unité de Biochimie Microbienne, Centre National de la Recherche Scientifique URA 1300, Institut Pasteur, Paris, France2.Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium3.Institut für Mikrobiologie, J. W. Göethe Universität, Frankfurt, Germany4.Unité de Lutte Biologique, Institut National de la Recherche Agronomique, France5. Unité de Biochimie Microbienne, Centre National de la Recherche Scientifique URA 1300, Institut Pasteur, Paris, France2.Biotechnology Centre of Oslo and School of Pharmacy, University of Oslo, PO Box 1123, N-0349 Oslo, Norway1.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10589720

Citation

Økstad, Ole A., et al. "Sequence Analysis of Three Bacillus Cereus Loci Carrying PIcR-regulated Genes Encoding Degradative Enzymes and Enterotoxin." Microbiology (Reading, England), vol. 145 (Pt 11), 1999, pp. 3129-3138.
Økstad OA, Gominet M, Purnelle B, et al. Sequence analysis of three Bacillus cereus loci carrying PIcR-regulated genes encoding degradative enzymes and enterotoxin. Microbiology (Reading, Engl). 1999;145 (Pt 11):3129-3138.
Økstad, O. A., Gominet, M., Purnelle, B., Rose, M., Lereclus, D., & Kolstø, A. B. (1999). Sequence analysis of three Bacillus cereus loci carrying PIcR-regulated genes encoding degradative enzymes and enterotoxin. Microbiology (Reading, England), 145 (Pt 11), 3129-3138. https://doi.org/10.1099/00221287-145-11-3129
Økstad OA, et al. Sequence Analysis of Three Bacillus Cereus Loci Carrying PIcR-regulated Genes Encoding Degradative Enzymes and Enterotoxin. Microbiology (Reading, Engl). 1999;145 (Pt 11):3129-3138. PubMed PMID: 10589720.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence analysis of three Bacillus cereus loci carrying PIcR-regulated genes encoding degradative enzymes and enterotoxin. AU - Økstad,Ole A, AU - Gominet,Myriam, AU - Purnelle,Bénédicte, AU - Rose,Matthias, AU - Lereclus,Didier, AU - Kolstø,Anne-Brit, PY - 1999/12/10/pubmed PY - 1999/12/10/medline PY - 1999/12/10/entrez SP - 3129 EP - 3138 JF - Microbiology (Reading, England) JO - Microbiology (Reading, Engl.) VL - 145 (Pt 11) N2 - PIcR is a pleiotropic regulator of extracellular virulence factors in the opportunistic human pathogen Bacillus cereus and the entomopathogenic Bacillus thuringiensis, and is induced in cells entering stationary phase. Among the genes regulated by PIcR are: pIcA, encoding phosphatidylinositol-specific phospholipase C (PI-PLC); plc, encoding phosphatidylcholine-preferring phospholipase C (PC-PLC); nhe, encoding the non-haemolytic enterotoxin; hbl, encoding haemolytic enterotoxin BL (HBL); and genes specifying a putative S-layer like surface protein and a putative extracellular RNase. By analysing 37.1 kb of DNA sequence surrounding hbl, plcA and plcR, 28 ORFs were predicted. Three novel genes putatively regulated by PlcR and encoding a neutral protease (NprB), a subtilase family serine protease (Sfp) and a putative cell-wall hydrolase (Cwh) were identified. The corresponding sfp and cwh genes were located in the immediate upstream region of plcA and could both be regulated by a putative PlcR-binding site positioned between the inversely transcribed genes. Similarly, nprB was positioned directly upstream and transcribed in the opposite orientation to plcR. Genes surrounding plcA, plcR and hblCDAB that were lacking an upstream PlcR regulatory sequence did not appear to serve functions apparently related to PlcR and did not exhibit a conserved organization in Bacillus subtilis. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/10589720/Sequence_analysis_of_three_Bacillus_cereus_loci_carrying_PIcR_regulated_genes_encoding_degradative_enzymes_and_enterotoxin_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/00221287-145-11-3129 DB - PRIME DP - Unbound Medicine ER -