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[Detection of neutralizing antibodies to Japanese encephalitis virus by enzyme-linked immunosorbent assay (ELISA)].

Abstract

Competitive ELISA was used for the detection of neutralizing antibody to JE. Based on the principle that human serum JE antibody competed with JE monoclonal antibody (MAb) for JE antigen, it was found that 3 JE MAbs (E3-3, NPF-5 and NNN-5) were suitable for competitive ELISA for the detection of JE neutralizing antibody. The sensitivity of cometitive ELISA for 29 JE confirmed serum specimens with titer of plaque reduction neutralization test (PRNT) was checked to be 82.1% (23/28). The specificity of E3-3 MAb to JE used in competitive ELISA was 100%. Correlation coefficient of JE confirmed cases of 57 hemagglutination inhibition (HI) titers in 1995 and 37 PRNT titers in 1994 compared with competitive ELISA were 0.744 and 0.732, respectively. Compared the competitive ELISA titers of 154 sera of healthy people with PRNT titers, the results showed that 70% of the sera could be detected by competitive ELISA which saved a lot of time and manpower.

Authors+Show Affiliations

National Institute of Preventive Medicine, Department of Health, Executive Yuan, Taipei, Taiwan, R.O.C.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

10592829

Citation

Chow, L, et al. "[Detection of Neutralizing Antibodies to Japanese Encephalitis Virus By Enzyme-linked Immunosorbent Assay (ELISA)]." Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology, vol. 30, no. 4, 1997, pp. 255-64.
Chow L, Yueh YY, Tseng WL, et al. [Detection of neutralizing antibodies to Japanese encephalitis virus by enzyme-linked immunosorbent assay (ELISA)]. Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1997;30(4):255-64.
Chow, L., Yueh, Y. Y., Tseng, W. L., Wu, Y. C., Wu, S. C., & Horng, C. B. (1997). [Detection of neutralizing antibodies to Japanese encephalitis virus by enzyme-linked immunosorbent assay (ELISA)]. Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology, 30(4), 255-64.
Chow L, et al. [Detection of Neutralizing Antibodies to Japanese Encephalitis Virus By Enzyme-linked Immunosorbent Assay (ELISA)]. Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1997;30(4):255-64. PubMed PMID: 10592829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection of neutralizing antibodies to Japanese encephalitis virus by enzyme-linked immunosorbent assay (ELISA)]. AU - Chow,L, AU - Yueh,Y Y, AU - Tseng,W L, AU - Wu,Y C, AU - Wu,S C, AU - Horng,C B, PY - 1999/12/11/pubmed PY - 1999/12/11/medline PY - 1999/12/11/entrez SP - 255 EP - 64 JF - Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology JO - Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi VL - 30 IS - 4 N2 - Competitive ELISA was used for the detection of neutralizing antibody to JE. Based on the principle that human serum JE antibody competed with JE monoclonal antibody (MAb) for JE antigen, it was found that 3 JE MAbs (E3-3, NPF-5 and NNN-5) were suitable for competitive ELISA for the detection of JE neutralizing antibody. The sensitivity of cometitive ELISA for 29 JE confirmed serum specimens with titer of plaque reduction neutralization test (PRNT) was checked to be 82.1% (23/28). The specificity of E3-3 MAb to JE used in competitive ELISA was 100%. Correlation coefficient of JE confirmed cases of 57 hemagglutination inhibition (HI) titers in 1995 and 37 PRNT titers in 1994 compared with competitive ELISA were 0.744 and 0.732, respectively. Compared the competitive ELISA titers of 154 sera of healthy people with PRNT titers, the results showed that 70% of the sera could be detected by competitive ELISA which saved a lot of time and manpower. SN - 0253-2662 UR - https://www.unboundmedicine.com/medline/citation/10592829/[Detection_of_neutralizing_antibodies_to_Japanese_encephalitis_virus_by_enzyme_linked_immunosorbent_assay__ELISA_]_ L2 - http://www.diseaseinfosearch.org/result/3899 DB - PRIME DP - Unbound Medicine ER -