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Ca(2+)-ATPase activity in streptozotocin-induced diabetic rat kidneys.
Diabetes Metab. 1999 Nov; 25(5):399-403.DM

Abstract

Kidney Ca(2+)-ATPase activity was altered in streptozotocin (STZ)-induced diabetic rats. Male rats, 200-250 g, were rendered diabetic by injection of STZ 45 mg/kg body weight via the tail vein. Following injection, control rats and diabetic rats at 1, 4, 8 or 15 weeks were sacrificed. Kidney tissues were obtained for the isolation of Ca(2+)-ATPase. Ca(2+)-ATPase activity was determined spectrophotometrically. Total calcium was measured by atomic absorption spectrophotometry. Diabetic rats had significantly elevated blood glucose levels compared to controls. Blood glucose levels were 92.92 +/- 1.215 mg/dl (mean +/- S.E.M.) for the control group, and 362.50 +/- 9.613 mg/dl at one week and > 500 mg/dl at 4, 8 and 15 weeks of diabetes. Enzyme activities were significantly higher at 4, 8 and 15 weeks of diabetes than in the control group (p < 0.001). There was no significant increase at one week of diabetes. Ca(2+)-ATPase activity was 0.43 +/- 0.003 U/L, 0.517 +/- 0.058 U/L, 0.707 +/- 0.078 U/L, 0.730 +/- 0.006 U/L and 0.715 +/- 0.055 U/L respectively for controls and rats at 1, 4, 8 and 15 weeks of diabetes. Calcium levels in diabetic rat kidneys were also significantly higher than for controls. The increase in enzyme activity may have been caused by higher calcium levels in diabetic kidneys resulting from a compensatory response of the enzyme to high levels of the ion.

Authors+Show Affiliations

Department of Biochemistry, University of Ankara, Faculty of Pharmacy, Turkey. evcimen@pharmacy.ankara.edu.trNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10592862

Citation

Evcimen, N D., et al. "Ca(2+)-ATPase Activity in Streptozotocin-induced Diabetic Rat Kidneys." Diabetes & Metabolism, vol. 25, no. 5, 1999, pp. 399-403.
Evcimen ND, Pekiner BD, Nebioglu S. Ca(2+)-ATPase activity in streptozotocin-induced diabetic rat kidneys. Diabetes Metab. 1999;25(5):399-403.
Evcimen, N. D., Pekiner, B. D., & Nebioglu, S. (1999). Ca(2+)-ATPase activity in streptozotocin-induced diabetic rat kidneys. Diabetes & Metabolism, 25(5), 399-403.
Evcimen ND, Pekiner BD, Nebioglu S. Ca(2+)-ATPase Activity in Streptozotocin-induced Diabetic Rat Kidneys. Diabetes Metab. 1999;25(5):399-403. PubMed PMID: 10592862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ca(2+)-ATPase activity in streptozotocin-induced diabetic rat kidneys. AU - Evcimen,N D, AU - Pekiner,B D, AU - Nebioglu,S, PY - 1999/12/11/pubmed PY - 2000/3/11/medline PY - 1999/12/11/entrez SP - 399 EP - 403 JF - Diabetes & metabolism JO - Diabetes Metab VL - 25 IS - 5 N2 - Kidney Ca(2+)-ATPase activity was altered in streptozotocin (STZ)-induced diabetic rats. Male rats, 200-250 g, were rendered diabetic by injection of STZ 45 mg/kg body weight via the tail vein. Following injection, control rats and diabetic rats at 1, 4, 8 or 15 weeks were sacrificed. Kidney tissues were obtained for the isolation of Ca(2+)-ATPase. Ca(2+)-ATPase activity was determined spectrophotometrically. Total calcium was measured by atomic absorption spectrophotometry. Diabetic rats had significantly elevated blood glucose levels compared to controls. Blood glucose levels were 92.92 +/- 1.215 mg/dl (mean +/- S.E.M.) for the control group, and 362.50 +/- 9.613 mg/dl at one week and > 500 mg/dl at 4, 8 and 15 weeks of diabetes. Enzyme activities were significantly higher at 4, 8 and 15 weeks of diabetes than in the control group (p < 0.001). There was no significant increase at one week of diabetes. Ca(2+)-ATPase activity was 0.43 +/- 0.003 U/L, 0.517 +/- 0.058 U/L, 0.707 +/- 0.078 U/L, 0.730 +/- 0.006 U/L and 0.715 +/- 0.055 U/L respectively for controls and rats at 1, 4, 8 and 15 weeks of diabetes. Calcium levels in diabetic rat kidneys were also significantly higher than for controls. The increase in enzyme activity may have been caused by higher calcium levels in diabetic kidneys resulting from a compensatory response of the enzyme to high levels of the ion. SN - 1262-3636 UR - https://www.unboundmedicine.com/medline/citation/10592862/Ca_2+__ATPase_activity_in_streptozotocin_induced_diabetic_rat_kidneys_ DB - PRIME DP - Unbound Medicine ER -