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Increased flow of fatty acids toward beta-oxidation in developing seeds of Arabidopsis deficient in diacylglycerol acyltransferase activity or synthesizing medium-chain-length fatty acids.
Plant Physiol. 1999 Dec; 121(4):1359-66.PP

Abstract

Synthesis of polyhydroxyalkanoates (PHAs) from intermediates of fatty acid beta-oxidation was used as a tool to study fatty acid degradation in developing seeds of Arabidopsis. Transgenic plants expressing a peroxisomal PHA synthase under the control of a napin promoter accumulated PHA in developing seeds to a final level of 0. 06 mg g(-1) dry weight. In plants co-expressing a plastidial acyl-acyl carrier protein thioesterase from Cuphea lanceolata and a peroxisomal PHA synthase, approximately 18-fold more PHA accumulated in developing seeds. The proportion of 3-hydroxydecanoic acid monomer in the PHA was strongly increased, indicating a large flow of capric acid toward beta-oxidation. Furthermore, expression of the peroxisomal PHA synthase in an Arabidopsis mutant deficient in the enzyme diacylglycerol acyltransferase resulted in a 10-fold increase in PHA accumulation in developing seeds. These data indicate that plants can respond to the inadequate incorporation of fatty acids into triacylglycerides by recycling the fatty acids via beta-oxidation and that a considerable flow toward beta-oxidation can occur even in a plant tissue primarily devoted to the accumulation of storage lipids.

Authors+Show Affiliations

Institut d'Ecologie-Biologie et Physiologie Végétales, Bâtiment de Biologie, Université de Lausanne, CH-1015 Lausanne, Switzerland. yves.poirier@ie-bpv.unil.chNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10594123

Citation

Poirier, Y, et al. "Increased Flow of Fatty Acids Toward Beta-oxidation in Developing Seeds of Arabidopsis Deficient in Diacylglycerol Acyltransferase Activity or Synthesizing Medium-chain-length Fatty Acids." Plant Physiology, vol. 121, no. 4, 1999, pp. 1359-66.
Poirier Y, Ventre G, Caldelari D. Increased flow of fatty acids toward beta-oxidation in developing seeds of Arabidopsis deficient in diacylglycerol acyltransferase activity or synthesizing medium-chain-length fatty acids. Plant Physiol. 1999;121(4):1359-66.
Poirier, Y., Ventre, G., & Caldelari, D. (1999). Increased flow of fatty acids toward beta-oxidation in developing seeds of Arabidopsis deficient in diacylglycerol acyltransferase activity or synthesizing medium-chain-length fatty acids. Plant Physiology, 121(4), 1359-66.
Poirier Y, Ventre G, Caldelari D. Increased Flow of Fatty Acids Toward Beta-oxidation in Developing Seeds of Arabidopsis Deficient in Diacylglycerol Acyltransferase Activity or Synthesizing Medium-chain-length Fatty Acids. Plant Physiol. 1999;121(4):1359-66. PubMed PMID: 10594123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased flow of fatty acids toward beta-oxidation in developing seeds of Arabidopsis deficient in diacylglycerol acyltransferase activity or synthesizing medium-chain-length fatty acids. AU - Poirier,Y, AU - Ventre,G, AU - Caldelari,D, PY - 1999/12/14/pubmed PY - 1999/12/14/medline PY - 1999/12/14/entrez SP - 1359 EP - 66 JF - Plant physiology JO - Plant Physiol VL - 121 IS - 4 N2 - Synthesis of polyhydroxyalkanoates (PHAs) from intermediates of fatty acid beta-oxidation was used as a tool to study fatty acid degradation in developing seeds of Arabidopsis. Transgenic plants expressing a peroxisomal PHA synthase under the control of a napin promoter accumulated PHA in developing seeds to a final level of 0. 06 mg g(-1) dry weight. In plants co-expressing a plastidial acyl-acyl carrier protein thioesterase from Cuphea lanceolata and a peroxisomal PHA synthase, approximately 18-fold more PHA accumulated in developing seeds. The proportion of 3-hydroxydecanoic acid monomer in the PHA was strongly increased, indicating a large flow of capric acid toward beta-oxidation. Furthermore, expression of the peroxisomal PHA synthase in an Arabidopsis mutant deficient in the enzyme diacylglycerol acyltransferase resulted in a 10-fold increase in PHA accumulation in developing seeds. These data indicate that plants can respond to the inadequate incorporation of fatty acids into triacylglycerides by recycling the fatty acids via beta-oxidation and that a considerable flow toward beta-oxidation can occur even in a plant tissue primarily devoted to the accumulation of storage lipids. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/10594123/Increased_flow_of_fatty_acids_toward_beta_oxidation_in_developing_seeds_of_Arabidopsis_deficient_in_diacylglycerol_acyltransferase_activity_or_synthesizing_medium_chain_length_fatty_acids_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=10594123 DB - PRIME DP - Unbound Medicine ER -